Job ID = 16439880 SRX = SRX14894235 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:14:38 58927443 reads; of these: 58927443 (100.00%) were unpaired; of these: 3646844 (6.19%) aligned 0 times 46798768 (79.42%) aligned exactly 1 time 8481831 (14.39%) aligned >1 times 93.81% overall alignment rate Time searching: 00:14:39 Overall time: 00:14:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 42789921 / 55280599 = 0.7740 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:53:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:53:30: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:53:30: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:53:38: 1000000 INFO @ Tue, 02 Aug 2022 16:53:48: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:53:57: 3000000 INFO @ Tue, 02 Aug 2022 16:53:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:53:59: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:53:59: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:54:06: 4000000 INFO @ Tue, 02 Aug 2022 16:54:08: 1000000 INFO @ Tue, 02 Aug 2022 16:54:16: 5000000 INFO @ Tue, 02 Aug 2022 16:54:17: 2000000 INFO @ Tue, 02 Aug 2022 16:54:25: 6000000 INFO @ Tue, 02 Aug 2022 16:54:26: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:54:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:54:29: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:54:29: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:54:34: 4000000 INFO @ Tue, 02 Aug 2022 16:54:35: 7000000 INFO @ Tue, 02 Aug 2022 16:54:39: 1000000 INFO @ Tue, 02 Aug 2022 16:54:43: 5000000 INFO @ Tue, 02 Aug 2022 16:54:45: 8000000 INFO @ Tue, 02 Aug 2022 16:54:48: 2000000 INFO @ Tue, 02 Aug 2022 16:54:52: 6000000 INFO @ Tue, 02 Aug 2022 16:54:55: 9000000 INFO @ Tue, 02 Aug 2022 16:54:58: 3000000 INFO @ Tue, 02 Aug 2022 16:55:01: 7000000 INFO @ Tue, 02 Aug 2022 16:55:05: 10000000 INFO @ Tue, 02 Aug 2022 16:55:08: 4000000 INFO @ Tue, 02 Aug 2022 16:55:09: 8000000 INFO @ Tue, 02 Aug 2022 16:55:15: 11000000 INFO @ Tue, 02 Aug 2022 16:55:17: 5000000 INFO @ Tue, 02 Aug 2022 16:55:18: 9000000 INFO @ Tue, 02 Aug 2022 16:55:25: 12000000 INFO @ Tue, 02 Aug 2022 16:55:27: 6000000 INFO @ Tue, 02 Aug 2022 16:55:28: 10000000 INFO @ Tue, 02 Aug 2022 16:55:30: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:55:30: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:55:30: #1 total tags in treatment: 12490678 INFO @ Tue, 02 Aug 2022 16:55:30: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:55:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:55:31: #1 tags after filtering in treatment: 12490608 INFO @ Tue, 02 Aug 2022 16:55:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:55:31: #1 finished! INFO @ Tue, 02 Aug 2022 16:55:31: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:55:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:55:32: #2 number of paired peaks: 6255 INFO @ Tue, 02 Aug 2022 16:55:32: start model_add_line... INFO @ Tue, 02 Aug 2022 16:55:32: start X-correlation... INFO @ Tue, 02 Aug 2022 16:55:33: end of X-cor INFO @ Tue, 02 Aug 2022 16:55:33: #2 finished! INFO @ Tue, 02 Aug 2022 16:55:33: #2 predicted fragment length is 288 bps INFO @ Tue, 02 Aug 2022 16:55:33: #2 alternative fragment length(s) may be 288 bps INFO @ Tue, 02 Aug 2022 16:55:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.05_model.r INFO @ Tue, 02 Aug 2022 16:55:33: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:55:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:55:36: 11000000 INFO @ Tue, 02 Aug 2022 16:55:37: 7000000 INFO @ Tue, 02 Aug 2022 16:55:45: 12000000 INFO @ Tue, 02 Aug 2022 16:55:46: 8000000 INFO @ Tue, 02 Aug 2022 16:55:50: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:55:50: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:55:50: #1 total tags in treatment: 12490678 INFO @ Tue, 02 Aug 2022 16:55:50: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:55:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:55:50: #1 tags after filtering in treatment: 12490608 INFO @ Tue, 02 Aug 2022 16:55:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:55:50: #1 finished! INFO @ Tue, 02 Aug 2022 16:55:50: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:55:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:55:52: #2 number of paired peaks: 6255 INFO @ Tue, 02 Aug 2022 16:55:52: start model_add_line... INFO @ Tue, 02 Aug 2022 16:55:52: start X-correlation... INFO @ Tue, 02 Aug 2022 16:55:52: end of X-cor INFO @ Tue, 02 Aug 2022 16:55:52: #2 finished! INFO @ Tue, 02 Aug 2022 16:55:52: #2 predicted fragment length is 288 bps INFO @ Tue, 02 Aug 2022 16:55:52: #2 alternative fragment length(s) may be 288 bps INFO @ Tue, 02 Aug 2022 16:55:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.10_model.r INFO @ Tue, 02 Aug 2022 16:55:52: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:55:52: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:55:55: 9000000 INFO @ Tue, 02 Aug 2022 16:56:06: 10000000 INFO @ Tue, 02 Aug 2022 16:56:15: 11000000 INFO @ Tue, 02 Aug 2022 16:56:18: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:56:25: 12000000 INFO @ Tue, 02 Aug 2022 16:56:30: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:56:30: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:56:30: #1 total tags in treatment: 12490678 INFO @ Tue, 02 Aug 2022 16:56:30: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:56:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:56:31: #1 tags after filtering in treatment: 12490608 INFO @ Tue, 02 Aug 2022 16:56:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:56:31: #1 finished! INFO @ Tue, 02 Aug 2022 16:56:31: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:56:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:56:32: #2 number of paired peaks: 6255 INFO @ Tue, 02 Aug 2022 16:56:32: start model_add_line... INFO @ Tue, 02 Aug 2022 16:56:33: start X-correlation... INFO @ Tue, 02 Aug 2022 16:56:33: end of X-cor INFO @ Tue, 02 Aug 2022 16:56:33: #2 finished! INFO @ Tue, 02 Aug 2022 16:56:33: #2 predicted fragment length is 288 bps INFO @ Tue, 02 Aug 2022 16:56:33: #2 alternative fragment length(s) may be 288 bps INFO @ Tue, 02 Aug 2022 16:56:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.20_model.r INFO @ Tue, 02 Aug 2022 16:56:33: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:56:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:56:33: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.05_peaks.xls INFO @ Tue, 02 Aug 2022 16:56:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:56:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.05_summits.bed INFO @ Tue, 02 Aug 2022 16:56:34: Done! pass1 - making usageList (815 chroms): 3 millis pass2 - checking and writing primary data (10465 records, 4 fields): 100 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:56:36: #3 Call peaks for each chromosome... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:56:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.10_peaks.xls INFO @ Tue, 02 Aug 2022 16:56:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:56:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.10_summits.bed INFO @ Tue, 02 Aug 2022 16:56:50: Done! pass1 - making usageList (740 chroms): 10 millis pass2 - checking and writing primary data (8903 records, 4 fields): 87 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:57:18: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:57:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.20_peaks.xls INFO @ Tue, 02 Aug 2022 16:57:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:57:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14894235/SRX14894235.20_summits.bed INFO @ Tue, 02 Aug 2022 16:57:33: Done! pass1 - making usageList (585 chroms): 2 millis pass2 - checking and writing primary data (7127 records, 4 fields): 57 millis CompletedMACS2peakCalling