Job ID = 16439994 SRX = SRX14894232 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:46 44489333 reads; of these: 44489333 (100.00%) were unpaired; of these: 4248147 (9.55%) aligned 0 times 33152056 (74.52%) aligned exactly 1 time 7089130 (15.93%) aligned >1 times 90.45% overall alignment rate Time searching: 00:10:46 Overall time: 00:10:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 31709473 / 40241186 = 0.7880 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:51:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:51:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:51:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:51:33: 1000000 INFO @ Tue, 02 Aug 2022 16:51:40: 2000000 INFO @ Tue, 02 Aug 2022 16:51:46: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:51:53: 4000000 INFO @ Tue, 02 Aug 2022 16:51:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:51:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:51:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:52:01: 5000000 INFO @ Tue, 02 Aug 2022 16:52:03: 1000000 INFO @ Tue, 02 Aug 2022 16:52:08: 6000000 INFO @ Tue, 02 Aug 2022 16:52:10: 2000000 INFO @ Tue, 02 Aug 2022 16:52:15: 7000000 INFO @ Tue, 02 Aug 2022 16:52:17: 3000000 INFO @ Tue, 02 Aug 2022 16:52:23: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:52:25: 4000000 INFO @ Tue, 02 Aug 2022 16:52:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:52:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:52:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:52:27: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:52:27: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:52:27: #1 total tags in treatment: 8531713 INFO @ Tue, 02 Aug 2022 16:52:27: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:52:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:52:27: #1 tags after filtering in treatment: 8531633 INFO @ Tue, 02 Aug 2022 16:52:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:52:27: #1 finished! INFO @ Tue, 02 Aug 2022 16:52:27: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:52:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:52:29: #2 number of paired peaks: 5872 INFO @ Tue, 02 Aug 2022 16:52:29: start model_add_line... INFO @ Tue, 02 Aug 2022 16:52:29: start X-correlation... INFO @ Tue, 02 Aug 2022 16:52:29: end of X-cor INFO @ Tue, 02 Aug 2022 16:52:29: #2 finished! INFO @ Tue, 02 Aug 2022 16:52:29: #2 predicted fragment length is 256 bps INFO @ Tue, 02 Aug 2022 16:52:29: #2 alternative fragment length(s) may be 256 bps INFO @ Tue, 02 Aug 2022 16:52:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.05_model.r INFO @ Tue, 02 Aug 2022 16:52:29: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:52:29: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:52:32: 5000000 INFO @ Tue, 02 Aug 2022 16:52:34: 1000000 INFO @ Tue, 02 Aug 2022 16:52:39: 6000000 INFO @ Tue, 02 Aug 2022 16:52:43: 2000000 INFO @ Tue, 02 Aug 2022 16:52:48: 7000000 INFO @ Tue, 02 Aug 2022 16:52:51: 3000000 INFO @ Tue, 02 Aug 2022 16:52:55: 8000000 INFO @ Tue, 02 Aug 2022 16:52:58: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:53:00: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:53:00: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:53:00: #1 total tags in treatment: 8531713 INFO @ Tue, 02 Aug 2022 16:53:00: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:53:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:53:00: 4000000 INFO @ Tue, 02 Aug 2022 16:53:00: #1 tags after filtering in treatment: 8531633 INFO @ Tue, 02 Aug 2022 16:53:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:53:00: #1 finished! INFO @ Tue, 02 Aug 2022 16:53:00: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:53:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:53:02: #2 number of paired peaks: 5872 INFO @ Tue, 02 Aug 2022 16:53:02: start model_add_line... INFO @ Tue, 02 Aug 2022 16:53:02: start X-correlation... INFO @ Tue, 02 Aug 2022 16:53:02: end of X-cor INFO @ Tue, 02 Aug 2022 16:53:02: #2 finished! INFO @ Tue, 02 Aug 2022 16:53:02: #2 predicted fragment length is 256 bps INFO @ Tue, 02 Aug 2022 16:53:02: #2 alternative fragment length(s) may be 256 bps INFO @ Tue, 02 Aug 2022 16:53:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.10_model.r INFO @ Tue, 02 Aug 2022 16:53:02: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:53:02: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:53:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.05_peaks.xls INFO @ Tue, 02 Aug 2022 16:53:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:53:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.05_summits.bed INFO @ Tue, 02 Aug 2022 16:53:08: Done! INFO @ Tue, 02 Aug 2022 16:53:09: 5000000 pass1 - making usageList (790 chroms): 2 millis pass2 - checking and writing primary data (6851 records, 4 fields): 76 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:53:17: 6000000 INFO @ Tue, 02 Aug 2022 16:53:26: 7000000 INFO @ Tue, 02 Aug 2022 16:53:31: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:53:35: 8000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:53:39: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:53:39: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:53:39: #1 total tags in treatment: 8531713 INFO @ Tue, 02 Aug 2022 16:53:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:53:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:53:40: #1 tags after filtering in treatment: 8531633 INFO @ Tue, 02 Aug 2022 16:53:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:53:40: #1 finished! INFO @ Tue, 02 Aug 2022 16:53:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:53:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:53:41: #2 number of paired peaks: 5872 INFO @ Tue, 02 Aug 2022 16:53:41: start model_add_line... INFO @ Tue, 02 Aug 2022 16:53:41: start X-correlation... INFO @ Tue, 02 Aug 2022 16:53:41: end of X-cor INFO @ Tue, 02 Aug 2022 16:53:41: #2 finished! INFO @ Tue, 02 Aug 2022 16:53:41: #2 predicted fragment length is 256 bps INFO @ Tue, 02 Aug 2022 16:53:41: #2 alternative fragment length(s) may be 256 bps INFO @ Tue, 02 Aug 2022 16:53:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.20_model.r INFO @ Tue, 02 Aug 2022 16:53:41: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:53:41: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:53:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.10_peaks.xls INFO @ Tue, 02 Aug 2022 16:53:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:53:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.10_summits.bed INFO @ Tue, 02 Aug 2022 16:53:42: Done! pass1 - making usageList (702 chroms): 3 millis pass2 - checking and writing primary data (5547 records, 4 fields): 108 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:54:10: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:54:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.20_peaks.xls INFO @ Tue, 02 Aug 2022 16:54:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:54:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14894232/SRX14894232.20_summits.bed INFO @ Tue, 02 Aug 2022 16:54:20: Done! pass1 - making usageList (583 chroms): 2 millis pass2 - checking and writing primary data (4322 records, 4 fields): 69 millis CompletedMACS2peakCalling