Job ID = 16439995 SRX = SRX14894230 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:51 65594084 reads; of these: 65594084 (100.00%) were unpaired; of these: 6731690 (10.26%) aligned 0 times 44158671 (67.32%) aligned exactly 1 time 14703723 (22.42%) aligned >1 times 89.74% overall alignment rate Time searching: 00:23:51 Overall time: 00:23:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 17629728 / 58862394 = 0.2995 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:11:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:11:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:11:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:12:06: 1000000 INFO @ Tue, 02 Aug 2022 17:12:15: 2000000 INFO @ Tue, 02 Aug 2022 17:12:23: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:12:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:12:26: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:12:26: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:12:32: 4000000 INFO @ Tue, 02 Aug 2022 17:12:35: 1000000 INFO @ Tue, 02 Aug 2022 17:12:40: 5000000 INFO @ Tue, 02 Aug 2022 17:12:44: 2000000 INFO @ Tue, 02 Aug 2022 17:12:49: 6000000 BedGraph に変換中... INFO @ Tue, 02 Aug 2022 17:12:54: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:12:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:12:56: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:12:56: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:12:57: 7000000 INFO @ Tue, 02 Aug 2022 17:13:03: 4000000 INFO @ Tue, 02 Aug 2022 17:13:04: 1000000 INFO @ Tue, 02 Aug 2022 17:13:05: 8000000 INFO @ Tue, 02 Aug 2022 17:13:12: 2000000 INFO @ Tue, 02 Aug 2022 17:13:13: 5000000 INFO @ Tue, 02 Aug 2022 17:13:14: 9000000 INFO @ Tue, 02 Aug 2022 17:13:20: 3000000 INFO @ Tue, 02 Aug 2022 17:13:22: 6000000 INFO @ Tue, 02 Aug 2022 17:13:22: 10000000 INFO @ Tue, 02 Aug 2022 17:13:27: 4000000 INFO @ Tue, 02 Aug 2022 17:13:31: 11000000 INFO @ Tue, 02 Aug 2022 17:13:32: 7000000 INFO @ Tue, 02 Aug 2022 17:13:35: 5000000 INFO @ Tue, 02 Aug 2022 17:13:39: 12000000 INFO @ Tue, 02 Aug 2022 17:13:42: 8000000 INFO @ Tue, 02 Aug 2022 17:13:43: 6000000 INFO @ Tue, 02 Aug 2022 17:13:48: 13000000 INFO @ Tue, 02 Aug 2022 17:13:50: 7000000 INFO @ Tue, 02 Aug 2022 17:13:51: 9000000 INFO @ Tue, 02 Aug 2022 17:13:56: 14000000 INFO @ Tue, 02 Aug 2022 17:13:58: 8000000 INFO @ Tue, 02 Aug 2022 17:14:01: 10000000 INFO @ Tue, 02 Aug 2022 17:14:04: 15000000 INFO @ Tue, 02 Aug 2022 17:14:06: 9000000 INFO @ Tue, 02 Aug 2022 17:14:10: 11000000 INFO @ Tue, 02 Aug 2022 17:14:12: 16000000 INFO @ Tue, 02 Aug 2022 17:14:14: 10000000 INFO @ Tue, 02 Aug 2022 17:14:19: 12000000 INFO @ Tue, 02 Aug 2022 17:14:20: 17000000 INFO @ Tue, 02 Aug 2022 17:14:22: 11000000 INFO @ Tue, 02 Aug 2022 17:14:28: 18000000 INFO @ Tue, 02 Aug 2022 17:14:29: 13000000 INFO @ Tue, 02 Aug 2022 17:14:29: 12000000 INFO @ Tue, 02 Aug 2022 17:14:35: 19000000 INFO @ Tue, 02 Aug 2022 17:14:36: 13000000 INFO @ Tue, 02 Aug 2022 17:14:38: 14000000 INFO @ Tue, 02 Aug 2022 17:14:44: 20000000 INFO @ Tue, 02 Aug 2022 17:14:44: 14000000 INFO @ Tue, 02 Aug 2022 17:14:47: 15000000 INFO @ Tue, 02 Aug 2022 17:14:51: 15000000 INFO @ Tue, 02 Aug 2022 17:14:52: 21000000 INFO @ Tue, 02 Aug 2022 17:14:56: 16000000 INFO @ Tue, 02 Aug 2022 17:14:59: 16000000 INFO @ Tue, 02 Aug 2022 17:15:00: 22000000 INFO @ Tue, 02 Aug 2022 17:15:04: 17000000 INFO @ Tue, 02 Aug 2022 17:15:06: 17000000 INFO @ Tue, 02 Aug 2022 17:15:08: 23000000 INFO @ Tue, 02 Aug 2022 17:15:13: 18000000 INFO @ Tue, 02 Aug 2022 17:15:13: 18000000 INFO @ Tue, 02 Aug 2022 17:15:16: 24000000 INFO @ Tue, 02 Aug 2022 17:15:21: 19000000 INFO @ Tue, 02 Aug 2022 17:15:22: 19000000 INFO @ Tue, 02 Aug 2022 17:15:24: 25000000 INFO @ Tue, 02 Aug 2022 17:15:28: 20000000 INFO @ Tue, 02 Aug 2022 17:15:31: 20000000 INFO @ Tue, 02 Aug 2022 17:15:32: 26000000 INFO @ Tue, 02 Aug 2022 17:15:36: 21000000 INFO @ Tue, 02 Aug 2022 17:15:39: 27000000 INFO @ Tue, 02 Aug 2022 17:15:39: 21000000 INFO @ Tue, 02 Aug 2022 17:15:43: 22000000 INFO @ Tue, 02 Aug 2022 17:15:47: 28000000 INFO @ Tue, 02 Aug 2022 17:15:48: 22000000 INFO @ Tue, 02 Aug 2022 17:15:50: 23000000 INFO @ Tue, 02 Aug 2022 17:15:55: 29000000 INFO @ Tue, 02 Aug 2022 17:15:57: 23000000 INFO @ Tue, 02 Aug 2022 17:15:57: 24000000 INFO @ Tue, 02 Aug 2022 17:16:03: 30000000 INFO @ Tue, 02 Aug 2022 17:16:04: 25000000 INFO @ Tue, 02 Aug 2022 17:16:05: 24000000 INFO @ Tue, 02 Aug 2022 17:16:10: 31000000 INFO @ Tue, 02 Aug 2022 17:16:11: 26000000 INFO @ Tue, 02 Aug 2022 17:16:14: 25000000 INFO @ Tue, 02 Aug 2022 17:16:18: 32000000 INFO @ Tue, 02 Aug 2022 17:16:19: 27000000 INFO @ Tue, 02 Aug 2022 17:16:23: 26000000 INFO @ Tue, 02 Aug 2022 17:16:26: 28000000 INFO @ Tue, 02 Aug 2022 17:16:27: 33000000 INFO @ Tue, 02 Aug 2022 17:16:31: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:16:33: 29000000 INFO @ Tue, 02 Aug 2022 17:16:35: 34000000 INFO @ Tue, 02 Aug 2022 17:16:40: 30000000 INFO @ Tue, 02 Aug 2022 17:16:41: 28000000 INFO @ Tue, 02 Aug 2022 17:16:43: 35000000 INFO @ Tue, 02 Aug 2022 17:16:47: 31000000 INFO @ Tue, 02 Aug 2022 17:16:50: 29000000 INFO @ Tue, 02 Aug 2022 17:16:52: 36000000 INFO @ Tue, 02 Aug 2022 17:16:54: 32000000 INFO @ Tue, 02 Aug 2022 17:16:59: 30000000 INFO @ Tue, 02 Aug 2022 17:17:00: 37000000 INFO @ Tue, 02 Aug 2022 17:17:02: 33000000 INFO @ Tue, 02 Aug 2022 17:17:08: 31000000 INFO @ Tue, 02 Aug 2022 17:17:09: 38000000 INFO @ Tue, 02 Aug 2022 17:17:09: 34000000 INFO @ Tue, 02 Aug 2022 17:17:16: 35000000 INFO @ Tue, 02 Aug 2022 17:17:17: 32000000 INFO @ Tue, 02 Aug 2022 17:17:17: 39000000 INFO @ Tue, 02 Aug 2022 17:17:24: 36000000 INFO @ Tue, 02 Aug 2022 17:17:26: 40000000 INFO @ Tue, 02 Aug 2022 17:17:27: 33000000 INFO @ Tue, 02 Aug 2022 17:17:31: 37000000 INFO @ Tue, 02 Aug 2022 17:17:34: 41000000 INFO @ Tue, 02 Aug 2022 17:17:35: 34000000 INFO @ Tue, 02 Aug 2022 17:17:37: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:17:37: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:17:37: #1 total tags in treatment: 41232666 INFO @ Tue, 02 Aug 2022 17:17:37: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:17:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:17:38: 38000000 INFO @ Tue, 02 Aug 2022 17:17:38: #1 tags after filtering in treatment: 41232615 INFO @ Tue, 02 Aug 2022 17:17:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:17:38: #1 finished! INFO @ Tue, 02 Aug 2022 17:17:38: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:17:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:17:42: #2 number of paired peaks: 20 WARNING @ Tue, 02 Aug 2022 17:17:42: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:17:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:17:44: 35000000 INFO @ Tue, 02 Aug 2022 17:17:45: 39000000 INFO @ Tue, 02 Aug 2022 17:17:52: 40000000 INFO @ Tue, 02 Aug 2022 17:17:53: 36000000 INFO @ Tue, 02 Aug 2022 17:18:00: 41000000 INFO @ Tue, 02 Aug 2022 17:18:03: 37000000 INFO @ Tue, 02 Aug 2022 17:18:03: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:18:03: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:18:03: #1 total tags in treatment: 41232666 INFO @ Tue, 02 Aug 2022 17:18:03: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:18:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:18:04: #1 tags after filtering in treatment: 41232615 INFO @ Tue, 02 Aug 2022 17:18:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:18:04: #1 finished! INFO @ Tue, 02 Aug 2022 17:18:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:18:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:18:07: #2 number of paired peaks: 20 WARNING @ Tue, 02 Aug 2022 17:18:07: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:18:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:18:11: 38000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 17:18:20: 39000000 INFO @ Tue, 02 Aug 2022 17:18:29: 40000000 INFO @ Tue, 02 Aug 2022 17:18:38: 41000000 INFO @ Tue, 02 Aug 2022 17:18:41: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:18:41: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:18:41: #1 total tags in treatment: 41232666 INFO @ Tue, 02 Aug 2022 17:18:41: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:18:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:18:42: #1 tags after filtering in treatment: 41232615 INFO @ Tue, 02 Aug 2022 17:18:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:18:42: #1 finished! INFO @ Tue, 02 Aug 2022 17:18:42: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:18:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:18:45: #2 number of paired peaks: 20 WARNING @ Tue, 02 Aug 2022 17:18:45: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:18:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894230/SRX14894230.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling