Job ID = 16439998 SRX = SRX14894226 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:18:56 63572032 reads; of these: 63572032 (100.00%) were unpaired; of these: 6106638 (9.61%) aligned 0 times 42860605 (67.42%) aligned exactly 1 time 14604789 (22.97%) aligned >1 times 90.39% overall alignment rate Time searching: 00:18:57 Overall time: 00:18:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 22140535 / 57465394 = 0.3853 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:07:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:07:00: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:07:00: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:07:09: 1000000 INFO @ Tue, 02 Aug 2022 17:07:18: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:07:27: 3000000 INFO @ Tue, 02 Aug 2022 17:07:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:07:29: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:07:29: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:07:36: 4000000 INFO @ Tue, 02 Aug 2022 17:07:38: 1000000 INFO @ Tue, 02 Aug 2022 17:07:45: 5000000 INFO @ Tue, 02 Aug 2022 17:07:47: 2000000 INFO @ Tue, 02 Aug 2022 17:07:55: 6000000 INFO @ Tue, 02 Aug 2022 17:07:56: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:07:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:07:59: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:07:59: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:08:04: 7000000 INFO @ Tue, 02 Aug 2022 17:08:05: 4000000 INFO @ Tue, 02 Aug 2022 17:08:10: 1000000 INFO @ Tue, 02 Aug 2022 17:08:13: 8000000 INFO @ Tue, 02 Aug 2022 17:08:14: 5000000 INFO @ Tue, 02 Aug 2022 17:08:19: 2000000 INFO @ Tue, 02 Aug 2022 17:08:22: 9000000 INFO @ Tue, 02 Aug 2022 17:08:23: 6000000 INFO @ Tue, 02 Aug 2022 17:08:29: 3000000 INFO @ Tue, 02 Aug 2022 17:08:31: 10000000 INFO @ Tue, 02 Aug 2022 17:08:33: 7000000 INFO @ Tue, 02 Aug 2022 17:08:38: 4000000 INFO @ Tue, 02 Aug 2022 17:08:40: 11000000 INFO @ Tue, 02 Aug 2022 17:08:41: 8000000 INFO @ Tue, 02 Aug 2022 17:08:47: 5000000 INFO @ Tue, 02 Aug 2022 17:08:48: 12000000 INFO @ Tue, 02 Aug 2022 17:08:50: 9000000 INFO @ Tue, 02 Aug 2022 17:09:02: 6000000 INFO @ Tue, 02 Aug 2022 17:09:03: 13000000 INFO @ Tue, 02 Aug 2022 17:09:05: 10000000 INFO @ Tue, 02 Aug 2022 17:09:11: 7000000 INFO @ Tue, 02 Aug 2022 17:09:12: 14000000 INFO @ Tue, 02 Aug 2022 17:09:14: 11000000 INFO @ Tue, 02 Aug 2022 17:09:20: 8000000 INFO @ Tue, 02 Aug 2022 17:09:21: 15000000 INFO @ Tue, 02 Aug 2022 17:09:23: 12000000 INFO @ Tue, 02 Aug 2022 17:09:29: 9000000 INFO @ Tue, 02 Aug 2022 17:09:30: 16000000 INFO @ Tue, 02 Aug 2022 17:09:32: 13000000 INFO @ Tue, 02 Aug 2022 17:09:39: 10000000 INFO @ Tue, 02 Aug 2022 17:09:39: 17000000 INFO @ Tue, 02 Aug 2022 17:09:41: 14000000 INFO @ Tue, 02 Aug 2022 17:09:47: 11000000 INFO @ Tue, 02 Aug 2022 17:09:48: 18000000 INFO @ Tue, 02 Aug 2022 17:09:50: 15000000 INFO @ Tue, 02 Aug 2022 17:09:56: 12000000 INFO @ Tue, 02 Aug 2022 17:09:57: 19000000 INFO @ Tue, 02 Aug 2022 17:10:00: 16000000 INFO @ Tue, 02 Aug 2022 17:10:05: 13000000 INFO @ Tue, 02 Aug 2022 17:10:06: 20000000 INFO @ Tue, 02 Aug 2022 17:10:09: 17000000 INFO @ Tue, 02 Aug 2022 17:10:15: 14000000 INFO @ Tue, 02 Aug 2022 17:10:15: 21000000 INFO @ Tue, 02 Aug 2022 17:10:18: 18000000 INFO @ Tue, 02 Aug 2022 17:10:24: 15000000 INFO @ Tue, 02 Aug 2022 17:10:24: 22000000 INFO @ Tue, 02 Aug 2022 17:10:28: 19000000 INFO @ Tue, 02 Aug 2022 17:10:33: 16000000 INFO @ Tue, 02 Aug 2022 17:10:33: 23000000 INFO @ Tue, 02 Aug 2022 17:10:37: 20000000 INFO @ Tue, 02 Aug 2022 17:10:42: 17000000 INFO @ Tue, 02 Aug 2022 17:10:43: 24000000 INFO @ Tue, 02 Aug 2022 17:10:47: 21000000 INFO @ Tue, 02 Aug 2022 17:10:51: 18000000 INFO @ Tue, 02 Aug 2022 17:10:52: 25000000 INFO @ Tue, 02 Aug 2022 17:10:56: 22000000 INFO @ Tue, 02 Aug 2022 17:11:00: 19000000 INFO @ Tue, 02 Aug 2022 17:11:01: 26000000 INFO @ Tue, 02 Aug 2022 17:11:05: 23000000 INFO @ Tue, 02 Aug 2022 17:11:09: 20000000 INFO @ Tue, 02 Aug 2022 17:11:10: 27000000 INFO @ Tue, 02 Aug 2022 17:11:14: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:11:18: 21000000 INFO @ Tue, 02 Aug 2022 17:11:19: 28000000 INFO @ Tue, 02 Aug 2022 17:11:24: 25000000 INFO @ Tue, 02 Aug 2022 17:11:28: 22000000 INFO @ Tue, 02 Aug 2022 17:11:29: 29000000 INFO @ Tue, 02 Aug 2022 17:11:33: 26000000 INFO @ Tue, 02 Aug 2022 17:11:37: 23000000 INFO @ Tue, 02 Aug 2022 17:11:38: 30000000 INFO @ Tue, 02 Aug 2022 17:11:43: 27000000 INFO @ Tue, 02 Aug 2022 17:11:46: 24000000 INFO @ Tue, 02 Aug 2022 17:11:48: 31000000 INFO @ Tue, 02 Aug 2022 17:11:52: 28000000 INFO @ Tue, 02 Aug 2022 17:11:55: 25000000 INFO @ Tue, 02 Aug 2022 17:11:57: 32000000 INFO @ Tue, 02 Aug 2022 17:12:02: 29000000 INFO @ Tue, 02 Aug 2022 17:12:04: 26000000 INFO @ Tue, 02 Aug 2022 17:12:06: 33000000 INFO @ Tue, 02 Aug 2022 17:12:11: 30000000 INFO @ Tue, 02 Aug 2022 17:12:13: 27000000 INFO @ Tue, 02 Aug 2022 17:12:15: 34000000 INFO @ Tue, 02 Aug 2022 17:12:21: 31000000 INFO @ Tue, 02 Aug 2022 17:12:22: 28000000 INFO @ Tue, 02 Aug 2022 17:12:25: 35000000 INFO @ Tue, 02 Aug 2022 17:12:28: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:12:28: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:12:28: #1 total tags in treatment: 35324859 INFO @ Tue, 02 Aug 2022 17:12:28: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:12:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:12:29: #1 tags after filtering in treatment: 35324810 INFO @ Tue, 02 Aug 2022 17:12:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:12:29: #1 finished! INFO @ Tue, 02 Aug 2022 17:12:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:12:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:12:30: 32000000 INFO @ Tue, 02 Aug 2022 17:12:32: #2 number of paired peaks: 86 WARNING @ Tue, 02 Aug 2022 17:12:32: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:12:32: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 17:12:32: 29000000 cut: /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:12:39: 33000000 INFO @ Tue, 02 Aug 2022 17:12:41: 30000000 INFO @ Tue, 02 Aug 2022 17:12:48: 34000000 INFO @ Tue, 02 Aug 2022 17:12:51: 31000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 17:12:57: 35000000 INFO @ Tue, 02 Aug 2022 17:13:00: 32000000 INFO @ Tue, 02 Aug 2022 17:13:01: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:13:01: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:13:01: #1 total tags in treatment: 35324859 INFO @ Tue, 02 Aug 2022 17:13:01: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:13:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:13:02: #1 tags after filtering in treatment: 35324810 INFO @ Tue, 02 Aug 2022 17:13:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:13:02: #1 finished! INFO @ Tue, 02 Aug 2022 17:13:02: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:13:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:13:04: #2 number of paired peaks: 86 WARNING @ Tue, 02 Aug 2022 17:13:04: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:13:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:13:09: 33000000 INFO @ Tue, 02 Aug 2022 17:13:19: 34000000 INFO @ Tue, 02 Aug 2022 17:13:28: 35000000 INFO @ Tue, 02 Aug 2022 17:13:31: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:13:31: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:13:31: #1 total tags in treatment: 35324859 INFO @ Tue, 02 Aug 2022 17:13:31: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:13:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:13:32: #1 tags after filtering in treatment: 35324810 INFO @ Tue, 02 Aug 2022 17:13:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:13:33: #1 finished! INFO @ Tue, 02 Aug 2022 17:13:33: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:13:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:13:35: #2 number of paired peaks: 86 WARNING @ Tue, 02 Aug 2022 17:13:35: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:13:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894226/SRX14894226.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling