Job ID = 16439872 SRX = SRX14894223 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:29 55739158 reads; of these: 55739158 (100.00%) were unpaired; of these: 4204124 (7.54%) aligned 0 times 38016613 (68.20%) aligned exactly 1 time 13518421 (24.25%) aligned >1 times 92.46% overall alignment rate Time searching: 00:16:29 Overall time: 00:16:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 14786871 / 51535034 = 0.2869 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:55:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:55:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:55:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:55:10: 1000000 INFO @ Tue, 02 Aug 2022 16:55:15: 2000000 INFO @ Tue, 02 Aug 2022 16:55:21: 3000000 INFO @ Tue, 02 Aug 2022 16:55:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:55:32: 5000000 INFO @ Tue, 02 Aug 2022 16:55:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:55:34: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:55:34: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:55:38: 6000000 INFO @ Tue, 02 Aug 2022 16:55:40: 1000000 INFO @ Tue, 02 Aug 2022 16:55:44: 7000000 INFO @ Tue, 02 Aug 2022 16:55:47: 2000000 INFO @ Tue, 02 Aug 2022 16:55:50: 8000000 INFO @ Tue, 02 Aug 2022 16:55:54: 3000000 INFO @ Tue, 02 Aug 2022 16:55:57: 9000000 INFO @ Tue, 02 Aug 2022 16:56:01: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:56:03: 10000000 INFO @ Tue, 02 Aug 2022 16:56:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:56:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:56:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:56:07: 5000000 INFO @ Tue, 02 Aug 2022 16:56:10: 11000000 INFO @ Tue, 02 Aug 2022 16:56:11: 1000000 INFO @ Tue, 02 Aug 2022 16:56:14: 6000000 INFO @ Tue, 02 Aug 2022 16:56:17: 12000000 INFO @ Tue, 02 Aug 2022 16:56:18: 2000000 INFO @ Tue, 02 Aug 2022 16:56:21: 7000000 INFO @ Tue, 02 Aug 2022 16:56:24: 13000000 INFO @ Tue, 02 Aug 2022 16:56:25: 3000000 INFO @ Tue, 02 Aug 2022 16:56:27: 8000000 INFO @ Tue, 02 Aug 2022 16:56:31: 14000000 INFO @ Tue, 02 Aug 2022 16:56:32: 4000000 INFO @ Tue, 02 Aug 2022 16:56:34: 9000000 INFO @ Tue, 02 Aug 2022 16:56:39: 15000000 INFO @ Tue, 02 Aug 2022 16:56:39: 5000000 INFO @ Tue, 02 Aug 2022 16:56:40: 10000000 INFO @ Tue, 02 Aug 2022 16:56:46: 6000000 INFO @ Tue, 02 Aug 2022 16:56:46: 16000000 INFO @ Tue, 02 Aug 2022 16:56:47: 11000000 INFO @ Tue, 02 Aug 2022 16:56:53: 7000000 INFO @ Tue, 02 Aug 2022 16:56:54: 17000000 INFO @ Tue, 02 Aug 2022 16:56:54: 12000000 INFO @ Tue, 02 Aug 2022 16:56:59: 8000000 INFO @ Tue, 02 Aug 2022 16:57:00: 13000000 INFO @ Tue, 02 Aug 2022 16:57:01: 18000000 INFO @ Tue, 02 Aug 2022 16:57:05: 9000000 INFO @ Tue, 02 Aug 2022 16:57:07: 14000000 INFO @ Tue, 02 Aug 2022 16:57:08: 19000000 INFO @ Tue, 02 Aug 2022 16:57:12: 10000000 INFO @ Tue, 02 Aug 2022 16:57:14: 15000000 INFO @ Tue, 02 Aug 2022 16:57:15: 20000000 INFO @ Tue, 02 Aug 2022 16:57:18: 11000000 INFO @ Tue, 02 Aug 2022 16:57:20: 16000000 INFO @ Tue, 02 Aug 2022 16:57:22: 21000000 INFO @ Tue, 02 Aug 2022 16:57:25: 12000000 INFO @ Tue, 02 Aug 2022 16:57:26: 17000000 INFO @ Tue, 02 Aug 2022 16:57:29: 22000000 INFO @ Tue, 02 Aug 2022 16:57:31: 13000000 INFO @ Tue, 02 Aug 2022 16:57:33: 18000000 INFO @ Tue, 02 Aug 2022 16:57:36: 23000000 INFO @ Tue, 02 Aug 2022 16:57:37: 14000000 INFO @ Tue, 02 Aug 2022 16:57:40: 19000000 INFO @ Tue, 02 Aug 2022 16:57:43: 24000000 INFO @ Tue, 02 Aug 2022 16:57:44: 15000000 INFO @ Tue, 02 Aug 2022 16:57:46: 20000000 INFO @ Tue, 02 Aug 2022 16:57:50: 16000000 INFO @ Tue, 02 Aug 2022 16:57:50: 25000000 INFO @ Tue, 02 Aug 2022 16:57:53: 21000000 INFO @ Tue, 02 Aug 2022 16:57:56: 17000000 INFO @ Tue, 02 Aug 2022 16:57:57: 26000000 INFO @ Tue, 02 Aug 2022 16:57:59: 22000000 INFO @ Tue, 02 Aug 2022 16:58:03: 18000000 INFO @ Tue, 02 Aug 2022 16:58:04: 27000000 INFO @ Tue, 02 Aug 2022 16:58:05: 23000000 INFO @ Tue, 02 Aug 2022 16:58:09: 19000000 INFO @ Tue, 02 Aug 2022 16:58:11: 28000000 INFO @ Tue, 02 Aug 2022 16:58:12: 24000000 INFO @ Tue, 02 Aug 2022 16:58:15: 20000000 INFO @ Tue, 02 Aug 2022 16:58:18: 25000000 INFO @ Tue, 02 Aug 2022 16:58:18: 29000000 INFO @ Tue, 02 Aug 2022 16:58:21: 21000000 INFO @ Tue, 02 Aug 2022 16:58:24: 26000000 INFO @ Tue, 02 Aug 2022 16:58:26: 30000000 INFO @ Tue, 02 Aug 2022 16:58:28: 22000000 INFO @ Tue, 02 Aug 2022 16:58:31: 27000000 INFO @ Tue, 02 Aug 2022 16:58:33: 31000000 INFO @ Tue, 02 Aug 2022 16:58:34: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:58:37: 28000000 INFO @ Tue, 02 Aug 2022 16:58:40: 32000000 INFO @ Tue, 02 Aug 2022 16:58:40: 24000000 INFO @ Tue, 02 Aug 2022 16:58:43: 29000000 INFO @ Tue, 02 Aug 2022 16:58:46: 25000000 INFO @ Tue, 02 Aug 2022 16:58:46: 33000000 INFO @ Tue, 02 Aug 2022 16:58:50: 30000000 INFO @ Tue, 02 Aug 2022 16:58:52: 26000000 INFO @ Tue, 02 Aug 2022 16:58:52: 34000000 INFO @ Tue, 02 Aug 2022 16:58:56: 31000000 INFO @ Tue, 02 Aug 2022 16:58:58: 27000000 INFO @ Tue, 02 Aug 2022 16:58:59: 35000000 INFO @ Tue, 02 Aug 2022 16:59:02: 32000000 INFO @ Tue, 02 Aug 2022 16:59:05: 28000000 INFO @ Tue, 02 Aug 2022 16:59:06: 36000000 INFO @ Tue, 02 Aug 2022 16:59:09: 33000000 INFO @ Tue, 02 Aug 2022 16:59:11: 29000000 INFO @ Tue, 02 Aug 2022 16:59:11: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:59:11: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:59:11: #1 total tags in treatment: 36748163 INFO @ Tue, 02 Aug 2022 16:59:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:59:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:59:12: #1 tags after filtering in treatment: 36748118 INFO @ Tue, 02 Aug 2022 16:59:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:59:12: #1 finished! INFO @ Tue, 02 Aug 2022 16:59:12: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:59:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:59:14: #2 number of paired peaks: 57 WARNING @ Tue, 02 Aug 2022 16:59:14: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:59:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 02 Aug 2022 16:59:15: 34000000 pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:59:18: 30000000 INFO @ Tue, 02 Aug 2022 16:59:22: 35000000 INFO @ Tue, 02 Aug 2022 16:59:24: 31000000 INFO @ Tue, 02 Aug 2022 16:59:28: 36000000 INFO @ Tue, 02 Aug 2022 16:59:30: 32000000 INFO @ Tue, 02 Aug 2022 16:59:33: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:59:33: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:59:33: #1 total tags in treatment: 36748163 INFO @ Tue, 02 Aug 2022 16:59:33: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:59:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:59:34: #1 tags after filtering in treatment: 36748118 INFO @ Tue, 02 Aug 2022 16:59:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:59:34: #1 finished! INFO @ Tue, 02 Aug 2022 16:59:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:59:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:59:36: #2 number of paired peaks: 57 WARNING @ Tue, 02 Aug 2022 16:59:36: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:59:36: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 16:59:36: 33000000 cut: /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:59:42: 34000000 INFO @ Tue, 02 Aug 2022 16:59:48: 35000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:59:54: 36000000 INFO @ Tue, 02 Aug 2022 16:59:59: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:59:59: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:59:59: #1 total tags in treatment: 36748163 INFO @ Tue, 02 Aug 2022 16:59:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:59:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:00:00: #1 tags after filtering in treatment: 36748118 INFO @ Tue, 02 Aug 2022 17:00:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:00:00: #1 finished! INFO @ Tue, 02 Aug 2022 17:00:00: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:00:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:00:03: #2 number of paired peaks: 57 WARNING @ Tue, 02 Aug 2022 17:00:03: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:00:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894223/SRX14894223.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling