Job ID = 16439870 SRX = SRX14894222 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:39 54351756 reads; of these: 54351756 (100.00%) were unpaired; of these: 3823481 (7.03%) aligned 0 times 37525426 (69.04%) aligned exactly 1 time 13002849 (23.92%) aligned >1 times 92.97% overall alignment rate Time searching: 00:16:39 Overall time: 00:16:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 14602777 / 50528275 = 0.2890 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:55:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:55:38: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:55:38: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:55:45: 1000000 INFO @ Tue, 02 Aug 2022 16:55:51: 2000000 INFO @ Tue, 02 Aug 2022 16:55:58: 3000000 INFO @ Tue, 02 Aug 2022 16:56:04: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:56:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:56:07: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:56:07: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:56:10: 5000000 INFO @ Tue, 02 Aug 2022 16:56:14: 1000000 INFO @ Tue, 02 Aug 2022 16:56:17: 6000000 INFO @ Tue, 02 Aug 2022 16:56:21: 2000000 INFO @ Tue, 02 Aug 2022 16:56:24: 7000000 INFO @ Tue, 02 Aug 2022 16:56:28: 3000000 INFO @ Tue, 02 Aug 2022 16:56:30: 8000000 INFO @ Tue, 02 Aug 2022 16:56:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:56:37: 9000000 INFO @ Tue, 02 Aug 2022 16:56:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:56:37: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:56:37: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:56:42: 5000000 INFO @ Tue, 02 Aug 2022 16:56:44: 10000000 INFO @ Tue, 02 Aug 2022 16:56:44: 1000000 INFO @ Tue, 02 Aug 2022 16:56:49: 6000000 INFO @ Tue, 02 Aug 2022 16:56:51: 11000000 INFO @ Tue, 02 Aug 2022 16:56:51: 2000000 INFO @ Tue, 02 Aug 2022 16:56:56: 7000000 INFO @ Tue, 02 Aug 2022 16:56:58: 12000000 INFO @ Tue, 02 Aug 2022 16:56:58: 3000000 INFO @ Tue, 02 Aug 2022 16:57:02: 8000000 INFO @ Tue, 02 Aug 2022 16:57:05: 4000000 INFO @ Tue, 02 Aug 2022 16:57:05: 13000000 INFO @ Tue, 02 Aug 2022 16:57:09: 9000000 INFO @ Tue, 02 Aug 2022 16:57:11: 5000000 INFO @ Tue, 02 Aug 2022 16:57:12: 14000000 INFO @ Tue, 02 Aug 2022 16:57:16: 10000000 INFO @ Tue, 02 Aug 2022 16:57:18: 6000000 INFO @ Tue, 02 Aug 2022 16:57:19: 15000000 INFO @ Tue, 02 Aug 2022 16:57:22: 11000000 INFO @ Tue, 02 Aug 2022 16:57:25: 7000000 INFO @ Tue, 02 Aug 2022 16:57:25: 16000000 INFO @ Tue, 02 Aug 2022 16:57:29: 12000000 INFO @ Tue, 02 Aug 2022 16:57:31: 8000000 INFO @ Tue, 02 Aug 2022 16:57:32: 17000000 INFO @ Tue, 02 Aug 2022 16:57:35: 13000000 INFO @ Tue, 02 Aug 2022 16:57:38: 9000000 INFO @ Tue, 02 Aug 2022 16:57:39: 18000000 INFO @ Tue, 02 Aug 2022 16:57:42: 14000000 INFO @ Tue, 02 Aug 2022 16:57:45: 10000000 INFO @ Tue, 02 Aug 2022 16:57:46: 19000000 INFO @ Tue, 02 Aug 2022 16:57:49: 15000000 INFO @ Tue, 02 Aug 2022 16:57:52: 11000000 INFO @ Tue, 02 Aug 2022 16:57:53: 20000000 INFO @ Tue, 02 Aug 2022 16:57:56: 16000000 INFO @ Tue, 02 Aug 2022 16:57:58: 12000000 INFO @ Tue, 02 Aug 2022 16:58:00: 21000000 INFO @ Tue, 02 Aug 2022 16:58:03: 17000000 INFO @ Tue, 02 Aug 2022 16:58:05: 13000000 INFO @ Tue, 02 Aug 2022 16:58:06: 22000000 INFO @ Tue, 02 Aug 2022 16:58:10: 18000000 INFO @ Tue, 02 Aug 2022 16:58:12: 14000000 INFO @ Tue, 02 Aug 2022 16:58:13: 23000000 INFO @ Tue, 02 Aug 2022 16:58:17: 19000000 INFO @ Tue, 02 Aug 2022 16:58:19: 15000000 INFO @ Tue, 02 Aug 2022 16:58:20: 24000000 INFO @ Tue, 02 Aug 2022 16:58:24: 20000000 INFO @ Tue, 02 Aug 2022 16:58:26: 16000000 INFO @ Tue, 02 Aug 2022 16:58:26: 25000000 INFO @ Tue, 02 Aug 2022 16:58:31: 21000000 INFO @ Tue, 02 Aug 2022 16:58:32: 17000000 INFO @ Tue, 02 Aug 2022 16:58:33: 26000000 INFO @ Tue, 02 Aug 2022 16:58:38: 22000000 INFO @ Tue, 02 Aug 2022 16:58:39: 18000000 INFO @ Tue, 02 Aug 2022 16:58:40: 27000000 INFO @ Tue, 02 Aug 2022 16:58:45: 23000000 INFO @ Tue, 02 Aug 2022 16:58:46: 19000000 INFO @ Tue, 02 Aug 2022 16:58:47: 28000000 INFO @ Tue, 02 Aug 2022 16:58:52: 24000000 INFO @ Tue, 02 Aug 2022 16:58:53: 20000000 INFO @ Tue, 02 Aug 2022 16:58:54: 29000000 INFO @ Tue, 02 Aug 2022 16:58:59: 25000000 INFO @ Tue, 02 Aug 2022 16:58:59: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:59:01: 30000000 INFO @ Tue, 02 Aug 2022 16:59:06: 22000000 INFO @ Tue, 02 Aug 2022 16:59:06: 26000000 INFO @ Tue, 02 Aug 2022 16:59:08: 31000000 INFO @ Tue, 02 Aug 2022 16:59:13: 23000000 INFO @ Tue, 02 Aug 2022 16:59:13: 27000000 INFO @ Tue, 02 Aug 2022 16:59:15: 32000000 INFO @ Tue, 02 Aug 2022 16:59:20: 24000000 INFO @ Tue, 02 Aug 2022 16:59:20: 28000000 INFO @ Tue, 02 Aug 2022 16:59:22: 33000000 INFO @ Tue, 02 Aug 2022 16:59:27: 25000000 INFO @ Tue, 02 Aug 2022 16:59:28: 29000000 INFO @ Tue, 02 Aug 2022 16:59:29: 34000000 INFO @ Tue, 02 Aug 2022 16:59:34: 26000000 INFO @ Tue, 02 Aug 2022 16:59:35: 30000000 INFO @ Tue, 02 Aug 2022 16:59:36: 35000000 INFO @ Tue, 02 Aug 2022 16:59:41: 27000000 INFO @ Tue, 02 Aug 2022 16:59:42: 31000000 INFO @ Tue, 02 Aug 2022 16:59:43: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:59:43: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:59:43: #1 total tags in treatment: 35925498 INFO @ Tue, 02 Aug 2022 16:59:43: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:59:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:59:44: #1 tags after filtering in treatment: 35925457 INFO @ Tue, 02 Aug 2022 16:59:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:59:44: #1 finished! INFO @ Tue, 02 Aug 2022 16:59:44: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:59:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:59:47: #2 number of paired peaks: 50 WARNING @ Tue, 02 Aug 2022 16:59:47: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:59:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:59:48: 28000000 INFO @ Tue, 02 Aug 2022 16:59:50: 32000000 INFO @ Tue, 02 Aug 2022 16:59:56: 29000000 INFO @ Tue, 02 Aug 2022 16:59:57: 33000000 INFO @ Tue, 02 Aug 2022 17:00:03: 30000000 INFO @ Tue, 02 Aug 2022 17:00:04: 34000000 INFO @ Tue, 02 Aug 2022 17:00:10: 31000000 INFO @ Tue, 02 Aug 2022 17:00:11: 35000000 INFO @ Tue, 02 Aug 2022 17:00:17: 32000000 INFO @ Tue, 02 Aug 2022 17:00:19: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:00:19: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:00:19: #1 total tags in treatment: 35925498 INFO @ Tue, 02 Aug 2022 17:00:19: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:00:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:00:19: #1 tags after filtering in treatment: 35925457 INFO @ Tue, 02 Aug 2022 17:00:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:00:19: #1 finished! INFO @ Tue, 02 Aug 2022 17:00:19: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:00:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:00:22: #2 number of paired peaks: 50 WARNING @ Tue, 02 Aug 2022 17:00:22: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:00:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:00:24: 33000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 17:00:31: 34000000 INFO @ Tue, 02 Aug 2022 17:00:38: 35000000 INFO @ Tue, 02 Aug 2022 17:00:44: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:00:44: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:00:44: #1 total tags in treatment: 35925498 INFO @ Tue, 02 Aug 2022 17:00:44: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:00:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:00:45: #1 tags after filtering in treatment: 35925457 INFO @ Tue, 02 Aug 2022 17:00:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:00:45: #1 finished! INFO @ Tue, 02 Aug 2022 17:00:45: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:00:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:00:48: #2 number of paired peaks: 50 WARNING @ Tue, 02 Aug 2022 17:00:48: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:00:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894222/SRX14894222.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling