Job ID = 16438700 SRX = SRX14748151 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:13 4307458 reads; of these: 4307458 (100.00%) were unpaired; of these: 294966 (6.85%) aligned 0 times 2801496 (65.04%) aligned exactly 1 time 1210996 (28.11%) aligned >1 times 93.15% overall alignment rate Time searching: 00:01:13 Overall time: 00:01:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 927371 / 4012492 = 0.2311 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:09:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:09:06: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:09:06: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:09:11: 1000000 INFO @ Tue, 02 Aug 2022 14:09:17: 2000000 INFO @ Tue, 02 Aug 2022 14:09:23: 3000000 INFO @ Tue, 02 Aug 2022 14:09:23: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 14:09:23: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 14:09:23: #1 total tags in treatment: 3085121 INFO @ Tue, 02 Aug 2022 14:09:23: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:09:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:09:23: #1 tags after filtering in treatment: 3084837 INFO @ Tue, 02 Aug 2022 14:09:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:09:23: #1 finished! INFO @ Tue, 02 Aug 2022 14:09:23: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:09:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:09:24: #2 number of paired peaks: 2761 INFO @ Tue, 02 Aug 2022 14:09:24: start model_add_line... INFO @ Tue, 02 Aug 2022 14:09:24: start X-correlation... INFO @ Tue, 02 Aug 2022 14:09:24: end of X-cor INFO @ Tue, 02 Aug 2022 14:09:24: #2 finished! INFO @ Tue, 02 Aug 2022 14:09:24: #2 predicted fragment length is 197 bps INFO @ Tue, 02 Aug 2022 14:09:24: #2 alternative fragment length(s) may be 197 bps INFO @ Tue, 02 Aug 2022 14:09:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.05_model.r INFO @ Tue, 02 Aug 2022 14:09:24: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:09:24: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:09:32: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:09:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.05_peaks.xls INFO @ Tue, 02 Aug 2022 14:09:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:09:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.05_summits.bed INFO @ Tue, 02 Aug 2022 14:09:36: Done! INFO @ Tue, 02 Aug 2022 14:09:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:09:36: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:09:36: #1 read treatment tags... pass1 - making usageList (469 chroms): 1 millis pass2 - checking and writing primary data (3135 records, 4 fields): 37 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:09:43: 1000000 INFO @ Tue, 02 Aug 2022 14:09:50: 2000000 INFO @ Tue, 02 Aug 2022 14:09:57: 3000000 INFO @ Tue, 02 Aug 2022 14:09:58: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 14:09:58: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 14:09:58: #1 total tags in treatment: 3085121 INFO @ Tue, 02 Aug 2022 14:09:58: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:09:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:09:58: #1 tags after filtering in treatment: 3084837 INFO @ Tue, 02 Aug 2022 14:09:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:09:58: #1 finished! INFO @ Tue, 02 Aug 2022 14:09:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:09:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:09:59: #2 number of paired peaks: 2761 INFO @ Tue, 02 Aug 2022 14:09:59: start model_add_line... INFO @ Tue, 02 Aug 2022 14:09:59: start X-correlation... INFO @ Tue, 02 Aug 2022 14:09:59: end of X-cor INFO @ Tue, 02 Aug 2022 14:09:59: #2 finished! INFO @ Tue, 02 Aug 2022 14:09:59: #2 predicted fragment length is 197 bps INFO @ Tue, 02 Aug 2022 14:09:59: #2 alternative fragment length(s) may be 197 bps INFO @ Tue, 02 Aug 2022 14:09:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.10_model.r INFO @ Tue, 02 Aug 2022 14:09:59: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:09:59: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:10:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:10:06: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:10:06: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:10:07: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:10:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.10_peaks.xls INFO @ Tue, 02 Aug 2022 14:10:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:10:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.10_summits.bed INFO @ Tue, 02 Aug 2022 14:10:11: 1000000 INFO @ Tue, 02 Aug 2022 14:10:11: Done! pass1 - making usageList (378 chroms): 2 millis pass2 - checking and writing primary data (2146 records, 4 fields): 113 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:10:17: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:10:23: 3000000 INFO @ Tue, 02 Aug 2022 14:10:23: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 14:10:23: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 14:10:23: #1 total tags in treatment: 3085121 INFO @ Tue, 02 Aug 2022 14:10:23: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:10:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:10:24: #1 tags after filtering in treatment: 3084837 INFO @ Tue, 02 Aug 2022 14:10:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:10:24: #1 finished! INFO @ Tue, 02 Aug 2022 14:10:24: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:10:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:10:24: #2 number of paired peaks: 2761 INFO @ Tue, 02 Aug 2022 14:10:24: start model_add_line... INFO @ Tue, 02 Aug 2022 14:10:24: start X-correlation... INFO @ Tue, 02 Aug 2022 14:10:24: end of X-cor INFO @ Tue, 02 Aug 2022 14:10:24: #2 finished! INFO @ Tue, 02 Aug 2022 14:10:24: #2 predicted fragment length is 197 bps INFO @ Tue, 02 Aug 2022 14:10:24: #2 alternative fragment length(s) may be 197 bps INFO @ Tue, 02 Aug 2022 14:10:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.20_model.r INFO @ Tue, 02 Aug 2022 14:10:24: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:10:24: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:10:33: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:10:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.20_peaks.xls INFO @ Tue, 02 Aug 2022 14:10:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:10:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14748151/SRX14748151.20_summits.bed INFO @ Tue, 02 Aug 2022 14:10:36: Done! pass1 - making usageList (163 chroms): 1 millis pass2 - checking and writing primary data (1309 records, 4 fields): 64 millis CompletedMACS2peakCalling