Job ID = 16439829 SRX = SRX14237854 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:46 30523636 reads; of these: 30523636 (100.00%) were unpaired; of these: 1648055 (5.40%) aligned 0 times 24526422 (80.35%) aligned exactly 1 time 4349159 (14.25%) aligned >1 times 94.60% overall alignment rate Time searching: 00:09:46 Overall time: 00:09:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5949784 / 28875581 = 0.2060 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:35:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:35:56: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:35:56: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:36:02: 1000000 INFO @ Tue, 02 Aug 2022 16:36:08: 2000000 INFO @ Tue, 02 Aug 2022 16:36:14: 3000000 INFO @ Tue, 02 Aug 2022 16:36:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:36:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:36:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:36:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:36:26: 5000000 INFO @ Tue, 02 Aug 2022 16:36:32: 1000000 INFO @ Tue, 02 Aug 2022 16:36:33: 6000000 INFO @ Tue, 02 Aug 2022 16:36:39: 2000000 INFO @ Tue, 02 Aug 2022 16:36:40: 7000000 INFO @ Tue, 02 Aug 2022 16:36:46: 3000000 INFO @ Tue, 02 Aug 2022 16:36:47: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:36:53: 4000000 INFO @ Tue, 02 Aug 2022 16:36:55: 9000000 INFO @ Tue, 02 Aug 2022 16:36:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:36:57: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:36:57: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:37:00: 5000000 INFO @ Tue, 02 Aug 2022 16:37:02: 10000000 INFO @ Tue, 02 Aug 2022 16:37:04: 1000000 INFO @ Tue, 02 Aug 2022 16:37:08: 6000000 INFO @ Tue, 02 Aug 2022 16:37:09: 11000000 INFO @ Tue, 02 Aug 2022 16:37:12: 2000000 INFO @ Tue, 02 Aug 2022 16:37:14: 7000000 INFO @ Tue, 02 Aug 2022 16:37:17: 12000000 INFO @ Tue, 02 Aug 2022 16:37:19: 3000000 INFO @ Tue, 02 Aug 2022 16:37:22: 8000000 INFO @ Tue, 02 Aug 2022 16:37:24: 13000000 INFO @ Tue, 02 Aug 2022 16:37:26: 4000000 INFO @ Tue, 02 Aug 2022 16:37:29: 9000000 INFO @ Tue, 02 Aug 2022 16:37:31: 14000000 INFO @ Tue, 02 Aug 2022 16:37:34: 5000000 INFO @ Tue, 02 Aug 2022 16:37:36: 10000000 INFO @ Tue, 02 Aug 2022 16:37:38: 15000000 INFO @ Tue, 02 Aug 2022 16:37:41: 6000000 INFO @ Tue, 02 Aug 2022 16:37:44: 11000000 INFO @ Tue, 02 Aug 2022 16:37:46: 16000000 INFO @ Tue, 02 Aug 2022 16:37:49: 7000000 INFO @ Tue, 02 Aug 2022 16:37:51: 12000000 INFO @ Tue, 02 Aug 2022 16:37:53: 17000000 INFO @ Tue, 02 Aug 2022 16:37:56: 8000000 INFO @ Tue, 02 Aug 2022 16:37:58: 13000000 INFO @ Tue, 02 Aug 2022 16:38:00: 18000000 INFO @ Tue, 02 Aug 2022 16:38:03: 9000000 INFO @ Tue, 02 Aug 2022 16:38:05: 14000000 INFO @ Tue, 02 Aug 2022 16:38:08: 19000000 INFO @ Tue, 02 Aug 2022 16:38:11: 10000000 INFO @ Tue, 02 Aug 2022 16:38:13: 15000000 INFO @ Tue, 02 Aug 2022 16:38:16: 20000000 INFO @ Tue, 02 Aug 2022 16:38:18: 11000000 INFO @ Tue, 02 Aug 2022 16:38:20: 16000000 INFO @ Tue, 02 Aug 2022 16:38:23: 21000000 INFO @ Tue, 02 Aug 2022 16:38:25: 12000000 INFO @ Tue, 02 Aug 2022 16:38:27: 17000000 INFO @ Tue, 02 Aug 2022 16:38:30: 22000000 INFO @ Tue, 02 Aug 2022 16:38:32: 13000000 INFO @ Tue, 02 Aug 2022 16:38:34: 18000000 INFO @ Tue, 02 Aug 2022 16:38:38: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:38:38: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:38:38: #1 total tags in treatment: 22925797 INFO @ Tue, 02 Aug 2022 16:38:38: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:38:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:38:39: #1 tags after filtering in treatment: 22925797 INFO @ Tue, 02 Aug 2022 16:38:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:38:39: #1 finished! INFO @ Tue, 02 Aug 2022 16:38:39: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:38:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:38:40: 14000000 INFO @ Tue, 02 Aug 2022 16:38:41: #2 number of paired peaks: 2899 INFO @ Tue, 02 Aug 2022 16:38:41: start model_add_line... INFO @ Tue, 02 Aug 2022 16:38:41: start X-correlation... INFO @ Tue, 02 Aug 2022 16:38:41: end of X-cor INFO @ Tue, 02 Aug 2022 16:38:41: #2 finished! INFO @ Tue, 02 Aug 2022 16:38:41: #2 predicted fragment length is 211 bps INFO @ Tue, 02 Aug 2022 16:38:41: #2 alternative fragment length(s) may be 211 bps INFO @ Tue, 02 Aug 2022 16:38:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.05_model.r INFO @ Tue, 02 Aug 2022 16:38:41: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:38:41: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:38:42: 19000000 INFO @ Tue, 02 Aug 2022 16:38:46: 15000000 INFO @ Tue, 02 Aug 2022 16:38:49: 20000000 INFO @ Tue, 02 Aug 2022 16:38:53: 16000000 INFO @ Tue, 02 Aug 2022 16:38:56: 21000000 INFO @ Tue, 02 Aug 2022 16:39:00: 17000000 INFO @ Tue, 02 Aug 2022 16:39:03: 22000000 INFO @ Tue, 02 Aug 2022 16:39:07: 18000000 INFO @ Tue, 02 Aug 2022 16:39:10: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:39:10: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:39:10: #1 total tags in treatment: 22925797 INFO @ Tue, 02 Aug 2022 16:39:10: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:39:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:39:11: #1 tags after filtering in treatment: 22925797 INFO @ Tue, 02 Aug 2022 16:39:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:39:11: #1 finished! INFO @ Tue, 02 Aug 2022 16:39:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:39:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:39:13: #2 number of paired peaks: 2899 INFO @ Tue, 02 Aug 2022 16:39:13: start model_add_line... INFO @ Tue, 02 Aug 2022 16:39:14: start X-correlation... INFO @ Tue, 02 Aug 2022 16:39:14: end of X-cor INFO @ Tue, 02 Aug 2022 16:39:14: #2 finished! INFO @ Tue, 02 Aug 2022 16:39:14: #2 predicted fragment length is 211 bps INFO @ Tue, 02 Aug 2022 16:39:14: #2 alternative fragment length(s) may be 211 bps INFO @ Tue, 02 Aug 2022 16:39:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.10_model.r INFO @ Tue, 02 Aug 2022 16:39:14: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:39:14: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:39:14: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:39:21: 20000000 INFO @ Tue, 02 Aug 2022 16:39:27: 21000000 INFO @ Tue, 02 Aug 2022 16:39:33: 22000000 INFO @ Tue, 02 Aug 2022 16:39:40: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:39:40: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:39:40: #1 total tags in treatment: 22925797 INFO @ Tue, 02 Aug 2022 16:39:40: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:39:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:39:41: #1 tags after filtering in treatment: 22925797 INFO @ Tue, 02 Aug 2022 16:39:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:39:41: #1 finished! INFO @ Tue, 02 Aug 2022 16:39:41: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:39:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:39:42: #2 number of paired peaks: 2899 INFO @ Tue, 02 Aug 2022 16:39:42: start model_add_line... INFO @ Tue, 02 Aug 2022 16:39:43: start X-correlation... INFO @ Tue, 02 Aug 2022 16:39:43: end of X-cor INFO @ Tue, 02 Aug 2022 16:39:43: #2 finished! INFO @ Tue, 02 Aug 2022 16:39:43: #2 predicted fragment length is 211 bps INFO @ Tue, 02 Aug 2022 16:39:43: #2 alternative fragment length(s) may be 211 bps INFO @ Tue, 02 Aug 2022 16:39:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.20_model.r INFO @ Tue, 02 Aug 2022 16:39:43: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:39:43: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:39:44: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:40:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.05_peaks.xls INFO @ Tue, 02 Aug 2022 16:40:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:40:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.05_summits.bed INFO @ Tue, 02 Aug 2022 16:40:07: Done! pass1 - making usageList (367 chroms): 3 millis pass2 - checking and writing primary data (8336 records, 4 fields): 53 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:40:16: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:40:39: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.10_peaks.xls INFO @ Tue, 02 Aug 2022 16:40:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:40:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.10_summits.bed INFO @ Tue, 02 Aug 2022 16:40:39: Done! pass1 - making usageList (239 chroms): 2 millis pass2 - checking and writing primary data (6712 records, 4 fields): 40 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:40:44: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:41:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.20_peaks.xls INFO @ Tue, 02 Aug 2022 16:41:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:41:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14237854/SRX14237854.20_summits.bed INFO @ Tue, 02 Aug 2022 16:41:08: Done! pass1 - making usageList (135 chroms): 2 millis pass2 - checking and writing primary data (5095 records, 4 fields): 52 millis CompletedMACS2peakCalling