Job ID = 16439830 SRX = SRX14237852 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:43 30380946 reads; of these: 30380946 (100.00%) were unpaired; of these: 1613619 (5.31%) aligned 0 times 24330147 (80.08%) aligned exactly 1 time 4437180 (14.61%) aligned >1 times 94.69% overall alignment rate Time searching: 00:10:43 Overall time: 00:10:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5941288 / 28767327 = 0.2065 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:35:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:35:20: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:35:20: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:35:26: 1000000 INFO @ Tue, 02 Aug 2022 16:35:32: 2000000 INFO @ Tue, 02 Aug 2022 16:35:37: 3000000 INFO @ Tue, 02 Aug 2022 16:35:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:35:49: 5000000 INFO @ Tue, 02 Aug 2022 16:35:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:35:49: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:35:49: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:35:54: 6000000 INFO @ Tue, 02 Aug 2022 16:35:55: 1000000 INFO @ Tue, 02 Aug 2022 16:36:01: 7000000 INFO @ Tue, 02 Aug 2022 16:36:01: 2000000 INFO @ Tue, 02 Aug 2022 16:36:06: 8000000 INFO @ Tue, 02 Aug 2022 16:36:08: 3000000 INFO @ Tue, 02 Aug 2022 16:36:12: 9000000 INFO @ Tue, 02 Aug 2022 16:36:14: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:36:18: 10000000 INFO @ Tue, 02 Aug 2022 16:36:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:36:19: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:36:19: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:36:20: 5000000 INFO @ Tue, 02 Aug 2022 16:36:24: 11000000 INFO @ Tue, 02 Aug 2022 16:36:25: 1000000 INFO @ Tue, 02 Aug 2022 16:36:26: 6000000 INFO @ Tue, 02 Aug 2022 16:36:30: 12000000 INFO @ Tue, 02 Aug 2022 16:36:32: 2000000 INFO @ Tue, 02 Aug 2022 16:36:32: 7000000 INFO @ Tue, 02 Aug 2022 16:36:36: 13000000 INFO @ Tue, 02 Aug 2022 16:36:38: 3000000 INFO @ Tue, 02 Aug 2022 16:36:39: 8000000 INFO @ Tue, 02 Aug 2022 16:36:42: 14000000 INFO @ Tue, 02 Aug 2022 16:36:44: 4000000 INFO @ Tue, 02 Aug 2022 16:36:45: 9000000 INFO @ Tue, 02 Aug 2022 16:36:48: 15000000 INFO @ Tue, 02 Aug 2022 16:36:50: 5000000 INFO @ Tue, 02 Aug 2022 16:36:51: 10000000 INFO @ Tue, 02 Aug 2022 16:36:55: 16000000 INFO @ Tue, 02 Aug 2022 16:36:56: 6000000 INFO @ Tue, 02 Aug 2022 16:36:57: 11000000 INFO @ Tue, 02 Aug 2022 16:37:01: 17000000 INFO @ Tue, 02 Aug 2022 16:37:03: 7000000 INFO @ Tue, 02 Aug 2022 16:37:03: 12000000 INFO @ Tue, 02 Aug 2022 16:37:07: 18000000 INFO @ Tue, 02 Aug 2022 16:37:09: 8000000 INFO @ Tue, 02 Aug 2022 16:37:09: 13000000 INFO @ Tue, 02 Aug 2022 16:37:14: 19000000 INFO @ Tue, 02 Aug 2022 16:37:15: 9000000 INFO @ Tue, 02 Aug 2022 16:37:15: 14000000 INFO @ Tue, 02 Aug 2022 16:37:21: 20000000 INFO @ Tue, 02 Aug 2022 16:37:21: 10000000 INFO @ Tue, 02 Aug 2022 16:37:21: 15000000 INFO @ Tue, 02 Aug 2022 16:37:27: 11000000 INFO @ Tue, 02 Aug 2022 16:37:27: 16000000 INFO @ Tue, 02 Aug 2022 16:37:27: 21000000 INFO @ Tue, 02 Aug 2022 16:37:33: 12000000 INFO @ Tue, 02 Aug 2022 16:37:33: 17000000 INFO @ Tue, 02 Aug 2022 16:37:34: 22000000 INFO @ Tue, 02 Aug 2022 16:37:39: 13000000 INFO @ Tue, 02 Aug 2022 16:37:39: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:37:39: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:37:39: #1 total tags in treatment: 22826039 INFO @ Tue, 02 Aug 2022 16:37:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:37:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:37:40: 18000000 INFO @ Tue, 02 Aug 2022 16:37:40: #1 tags after filtering in treatment: 22826039 INFO @ Tue, 02 Aug 2022 16:37:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:37:40: #1 finished! INFO @ Tue, 02 Aug 2022 16:37:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:37:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:37:42: #2 number of paired peaks: 3198 INFO @ Tue, 02 Aug 2022 16:37:42: start model_add_line... INFO @ Tue, 02 Aug 2022 16:37:42: start X-correlation... INFO @ Tue, 02 Aug 2022 16:37:42: end of X-cor INFO @ Tue, 02 Aug 2022 16:37:42: #2 finished! INFO @ Tue, 02 Aug 2022 16:37:42: #2 predicted fragment length is 221 bps INFO @ Tue, 02 Aug 2022 16:37:42: #2 alternative fragment length(s) may be 221 bps INFO @ Tue, 02 Aug 2022 16:37:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.05_model.r INFO @ Tue, 02 Aug 2022 16:37:42: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:37:42: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:37:45: 14000000 INFO @ Tue, 02 Aug 2022 16:37:46: 19000000 INFO @ Tue, 02 Aug 2022 16:37:51: 15000000 INFO @ Tue, 02 Aug 2022 16:37:52: 20000000 INFO @ Tue, 02 Aug 2022 16:37:57: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:37:58: 21000000 INFO @ Tue, 02 Aug 2022 16:38:03: 17000000 INFO @ Tue, 02 Aug 2022 16:38:05: 22000000 INFO @ Tue, 02 Aug 2022 16:38:09: 18000000 INFO @ Tue, 02 Aug 2022 16:38:10: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:38:10: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:38:10: #1 total tags in treatment: 22826039 INFO @ Tue, 02 Aug 2022 16:38:10: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:38:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:38:11: #1 tags after filtering in treatment: 22826039 INFO @ Tue, 02 Aug 2022 16:38:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:38:11: #1 finished! INFO @ Tue, 02 Aug 2022 16:38:11: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:38:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:38:13: #2 number of paired peaks: 3198 INFO @ Tue, 02 Aug 2022 16:38:13: start model_add_line... INFO @ Tue, 02 Aug 2022 16:38:13: start X-correlation... INFO @ Tue, 02 Aug 2022 16:38:13: end of X-cor INFO @ Tue, 02 Aug 2022 16:38:13: #2 finished! INFO @ Tue, 02 Aug 2022 16:38:13: #2 predicted fragment length is 221 bps INFO @ Tue, 02 Aug 2022 16:38:13: #2 alternative fragment length(s) may be 221 bps INFO @ Tue, 02 Aug 2022 16:38:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.10_model.r INFO @ Tue, 02 Aug 2022 16:38:13: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:38:13: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:38:16: 19000000 INFO @ Tue, 02 Aug 2022 16:38:21: 20000000 INFO @ Tue, 02 Aug 2022 16:38:27: 21000000 INFO @ Tue, 02 Aug 2022 16:38:32: 22000000 INFO @ Tue, 02 Aug 2022 16:38:38: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 16:38:38: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 16:38:38: #1 total tags in treatment: 22826039 INFO @ Tue, 02 Aug 2022 16:38:38: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:38:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:38:38: #1 tags after filtering in treatment: 22826039 INFO @ Tue, 02 Aug 2022 16:38:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:38:38: #1 finished! INFO @ Tue, 02 Aug 2022 16:38:38: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:38:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:38:39: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:38:40: #2 number of paired peaks: 3198 INFO @ Tue, 02 Aug 2022 16:38:40: start model_add_line... INFO @ Tue, 02 Aug 2022 16:38:40: start X-correlation... INFO @ Tue, 02 Aug 2022 16:38:40: end of X-cor INFO @ Tue, 02 Aug 2022 16:38:40: #2 finished! INFO @ Tue, 02 Aug 2022 16:38:40: #2 predicted fragment length is 221 bps INFO @ Tue, 02 Aug 2022 16:38:40: #2 alternative fragment length(s) may be 221 bps INFO @ Tue, 02 Aug 2022 16:38:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.20_model.r INFO @ Tue, 02 Aug 2022 16:38:40: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:38:40: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:39:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.05_peaks.xls INFO @ Tue, 02 Aug 2022 16:39:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:39:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.05_summits.bed INFO @ Tue, 02 Aug 2022 16:39:03: Done! pass1 - making usageList (352 chroms): 2 millis pass2 - checking and writing primary data (8065 records, 4 fields): 53 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:39:09: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:39:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.10_peaks.xls INFO @ Tue, 02 Aug 2022 16:39:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:39:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.10_summits.bed INFO @ Tue, 02 Aug 2022 16:39:34: Done! pass1 - making usageList (250 chroms): 2 millis pass2 - checking and writing primary data (6572 records, 4 fields): 48 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:39:37: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:40:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.20_peaks.xls INFO @ Tue, 02 Aug 2022 16:40:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:40:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14237852/SRX14237852.20_summits.bed INFO @ Tue, 02 Aug 2022 16:40:01: Done! pass1 - making usageList (148 chroms): 2 millis pass2 - checking and writing primary data (4987 records, 4 fields): 43 millis CompletedMACS2peakCalling