Job ID = 6529298 SRX = SRX1361352 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:01 30868255 reads; of these: 30868255 (100.00%) were unpaired; of these: 2442640 (7.91%) aligned 0 times 13428465 (43.50%) aligned exactly 1 time 14997150 (48.58%) aligned >1 times 92.09% overall alignment rate Time searching: 00:10:01 Overall time: 00:10:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9965520 / 28425615 = 0.3506 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:54:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:54:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:54:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:54:50: 1000000 INFO @ Tue, 30 Jun 2020 01:54:56: 2000000 INFO @ Tue, 30 Jun 2020 01:55:01: 3000000 INFO @ Tue, 30 Jun 2020 01:55:07: 4000000 INFO @ Tue, 30 Jun 2020 01:55:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:55:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:55:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:55:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:55:18: 6000000 INFO @ Tue, 30 Jun 2020 01:55:22: 1000000 INFO @ Tue, 30 Jun 2020 01:55:24: 7000000 INFO @ Tue, 30 Jun 2020 01:55:28: 2000000 INFO @ Tue, 30 Jun 2020 01:55:30: 8000000 INFO @ Tue, 30 Jun 2020 01:55:34: 3000000 INFO @ Tue, 30 Jun 2020 01:55:37: 9000000 INFO @ Tue, 30 Jun 2020 01:55:41: 4000000 INFO @ Tue, 30 Jun 2020 01:55:43: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:55:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:55:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:55:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:55:47: 5000000 INFO @ Tue, 30 Jun 2020 01:55:49: 11000000 INFO @ Tue, 30 Jun 2020 01:55:52: 1000000 INFO @ Tue, 30 Jun 2020 01:55:53: 6000000 INFO @ Tue, 30 Jun 2020 01:55:56: 12000000 INFO @ Tue, 30 Jun 2020 01:55:58: 2000000 INFO @ Tue, 30 Jun 2020 01:56:00: 7000000 INFO @ Tue, 30 Jun 2020 01:56:02: 13000000 INFO @ Tue, 30 Jun 2020 01:56:04: 3000000 INFO @ Tue, 30 Jun 2020 01:56:06: 8000000 INFO @ Tue, 30 Jun 2020 01:56:09: 14000000 INFO @ Tue, 30 Jun 2020 01:56:10: 4000000 INFO @ Tue, 30 Jun 2020 01:56:12: 9000000 INFO @ Tue, 30 Jun 2020 01:56:15: 15000000 INFO @ Tue, 30 Jun 2020 01:56:17: 5000000 INFO @ Tue, 30 Jun 2020 01:56:18: 10000000 INFO @ Tue, 30 Jun 2020 01:56:22: 16000000 INFO @ Tue, 30 Jun 2020 01:56:23: 6000000 INFO @ Tue, 30 Jun 2020 01:56:25: 11000000 INFO @ Tue, 30 Jun 2020 01:56:28: 17000000 INFO @ Tue, 30 Jun 2020 01:56:29: 7000000 INFO @ Tue, 30 Jun 2020 01:56:31: 12000000 INFO @ Tue, 30 Jun 2020 01:56:34: 18000000 INFO @ Tue, 30 Jun 2020 01:56:35: 8000000 INFO @ Tue, 30 Jun 2020 01:56:37: 13000000 INFO @ Tue, 30 Jun 2020 01:56:37: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:56:37: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:56:37: #1 total tags in treatment: 18460095 INFO @ Tue, 30 Jun 2020 01:56:37: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:56:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:56:38: #1 tags after filtering in treatment: 18460055 INFO @ Tue, 30 Jun 2020 01:56:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:56:38: #1 finished! INFO @ Tue, 30 Jun 2020 01:56:38: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:56:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:56:39: #2 number of paired peaks: 2477 INFO @ Tue, 30 Jun 2020 01:56:39: start model_add_line... INFO @ Tue, 30 Jun 2020 01:56:39: start X-correlation... INFO @ Tue, 30 Jun 2020 01:56:39: end of X-cor INFO @ Tue, 30 Jun 2020 01:56:39: #2 finished! INFO @ Tue, 30 Jun 2020 01:56:39: #2 predicted fragment length is 2 bps INFO @ Tue, 30 Jun 2020 01:56:39: #2 alternative fragment length(s) may be 2,38 bps INFO @ Tue, 30 Jun 2020 01:56:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.05_model.r WARNING @ Tue, 30 Jun 2020 01:56:39: #2 Since the d (2) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 01:56:39: #2 You may need to consider one of the other alternative d(s): 2,38 WARNING @ Tue, 30 Jun 2020 01:56:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 01:56:39: #3 Call peaks... INFO @ Tue, 30 Jun 2020 01:56:39: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 01:56:41: 9000000 INFO @ Tue, 30 Jun 2020 01:56:44: 14000000 INFO @ Tue, 30 Jun 2020 01:56:47: 10000000 INFO @ Tue, 30 Jun 2020 01:56:50: 15000000 INFO @ Tue, 30 Jun 2020 01:56:54: 11000000 INFO @ Tue, 30 Jun 2020 01:56:56: 16000000 INFO @ Tue, 30 Jun 2020 01:57:00: 12000000 INFO @ Tue, 30 Jun 2020 01:57:02: 17000000 INFO @ Tue, 30 Jun 2020 01:57:06: 13000000 INFO @ Tue, 30 Jun 2020 01:57:08: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 01:57:08: 18000000 INFO @ Tue, 30 Jun 2020 01:57:11: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:57:11: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:57:11: #1 total tags in treatment: 18460095 INFO @ Tue, 30 Jun 2020 01:57:11: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:57:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:57:12: #1 tags after filtering in treatment: 18460055 INFO @ Tue, 30 Jun 2020 01:57:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:57:12: #1 finished! INFO @ Tue, 30 Jun 2020 01:57:12: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:57:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:57:12: 14000000 INFO @ Tue, 30 Jun 2020 01:57:13: #2 number of paired peaks: 2477 INFO @ Tue, 30 Jun 2020 01:57:13: start model_add_line... INFO @ Tue, 30 Jun 2020 01:57:13: start X-correlation... INFO @ Tue, 30 Jun 2020 01:57:13: end of X-cor INFO @ Tue, 30 Jun 2020 01:57:13: #2 finished! INFO @ Tue, 30 Jun 2020 01:57:13: #2 predicted fragment length is 2 bps INFO @ Tue, 30 Jun 2020 01:57:13: #2 alternative fragment length(s) may be 2,38 bps INFO @ Tue, 30 Jun 2020 01:57:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.10_model.r WARNING @ Tue, 30 Jun 2020 01:57:13: #2 Since the d (2) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 01:57:13: #2 You may need to consider one of the other alternative d(s): 2,38 WARNING @ Tue, 30 Jun 2020 01:57:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 01:57:13: #3 Call peaks... INFO @ Tue, 30 Jun 2020 01:57:13: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:57:18: 15000000 INFO @ Tue, 30 Jun 2020 01:57:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.05_peaks.xls INFO @ Tue, 30 Jun 2020 01:57:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.05_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 01:57:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.05_summits.bed INFO @ Tue, 30 Jun 2020 01:57:21: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:57:24: 16000000 INFO @ Tue, 30 Jun 2020 01:57:30: 17000000 INFO @ Tue, 30 Jun 2020 01:57:36: 18000000 INFO @ Tue, 30 Jun 2020 01:57:39: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:57:39: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:57:39: #1 total tags in treatment: 18460095 INFO @ Tue, 30 Jun 2020 01:57:39: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:57:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:57:39: #1 tags after filtering in treatment: 18460055 INFO @ Tue, 30 Jun 2020 01:57:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:57:39: #1 finished! INFO @ Tue, 30 Jun 2020 01:57:39: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:57:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:57:40: #2 number of paired peaks: 2477 INFO @ Tue, 30 Jun 2020 01:57:40: start model_add_line... INFO @ Tue, 30 Jun 2020 01:57:41: start X-correlation... INFO @ Tue, 30 Jun 2020 01:57:41: end of X-cor INFO @ Tue, 30 Jun 2020 01:57:41: #2 finished! INFO @ Tue, 30 Jun 2020 01:57:41: #2 predicted fragment length is 2 bps INFO @ Tue, 30 Jun 2020 01:57:41: #2 alternative fragment length(s) may be 2,38 bps INFO @ Tue, 30 Jun 2020 01:57:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.20_model.r WARNING @ Tue, 30 Jun 2020 01:57:41: #2 Since the d (2) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 01:57:41: #2 You may need to consider one of the other alternative d(s): 2,38 WARNING @ Tue, 30 Jun 2020 01:57:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 01:57:41: #3 Call peaks... INFO @ Tue, 30 Jun 2020 01:57:41: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 01:57:42: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 01:57:55: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.10_peaks.xls INFO @ Tue, 30 Jun 2020 01:57:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.10_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 01:57:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.10_summits.bed INFO @ Tue, 30 Jun 2020 01:57:55: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:58:09: #3 Call peaks for each chromosome... INFO @ Tue, 30 Jun 2020 01:58:22: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.20_peaks.xls INFO @ Tue, 30 Jun 2020 01:58:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.20_peaks.narrowPeak INFO @ Tue, 30 Jun 2020 01:58:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX1361352/SRX1361352.20_summits.bed INFO @ Tue, 30 Jun 2020 01:58:22: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling