Job ID = 6453667 SRX = SRX135529 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:39:02 prefetch.2.10.7: 1) Downloading 'SRR453254'... 2020-06-21T08:39:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:08:37 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:08:37 prefetch.2.10.7: 1) 'SRR453254' was downloaded successfully Read 109937835 spots for SRR453254/SRR453254.sra Written 109937835 spots for SRR453254/SRR453254.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:15 109937835 reads; of these: 109937835 (100.00%) were unpaired; of these: 41493148 (37.74%) aligned 0 times 59034714 (53.70%) aligned exactly 1 time 9409973 (8.56%) aligned >1 times 62.26% overall alignment rate Time searching: 00:22:15 Overall time: 00:22:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 36402894 / 68444687 = 0.5319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:49:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:49:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:49:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:49:35: 1000000 INFO @ Sun, 21 Jun 2020 18:49:42: 2000000 INFO @ Sun, 21 Jun 2020 18:49:49: 3000000 INFO @ Sun, 21 Jun 2020 18:49:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:49:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:49:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:49:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:50:03: 5000000 INFO @ Sun, 21 Jun 2020 18:50:06: 1000000 INFO @ Sun, 21 Jun 2020 18:50:11: 6000000 INFO @ Sun, 21 Jun 2020 18:50:13: 2000000 INFO @ Sun, 21 Jun 2020 18:50:19: 7000000 INFO @ Sun, 21 Jun 2020 18:50:21: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:50:27: 8000000 INFO @ Sun, 21 Jun 2020 18:50:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:50:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:50:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:50:29: 4000000 INFO @ Sun, 21 Jun 2020 18:50:35: 9000000 INFO @ Sun, 21 Jun 2020 18:50:36: 1000000 INFO @ Sun, 21 Jun 2020 18:50:36: 5000000 INFO @ Sun, 21 Jun 2020 18:50:42: 10000000 INFO @ Sun, 21 Jun 2020 18:50:44: 2000000 INFO @ Sun, 21 Jun 2020 18:50:44: 6000000 INFO @ Sun, 21 Jun 2020 18:50:50: 11000000 INFO @ Sun, 21 Jun 2020 18:50:52: 7000000 INFO @ Sun, 21 Jun 2020 18:50:52: 3000000 INFO @ Sun, 21 Jun 2020 18:50:58: 12000000 INFO @ Sun, 21 Jun 2020 18:51:00: 8000000 INFO @ Sun, 21 Jun 2020 18:51:00: 4000000 INFO @ Sun, 21 Jun 2020 18:51:06: 13000000 INFO @ Sun, 21 Jun 2020 18:51:08: 9000000 INFO @ Sun, 21 Jun 2020 18:51:08: 5000000 INFO @ Sun, 21 Jun 2020 18:51:14: 14000000 INFO @ Sun, 21 Jun 2020 18:51:15: 10000000 INFO @ Sun, 21 Jun 2020 18:51:16: 6000000 INFO @ Sun, 21 Jun 2020 18:51:22: 15000000 INFO @ Sun, 21 Jun 2020 18:51:23: 11000000 INFO @ Sun, 21 Jun 2020 18:51:24: 7000000 INFO @ Sun, 21 Jun 2020 18:51:30: 12000000 INFO @ Sun, 21 Jun 2020 18:51:30: 16000000 INFO @ Sun, 21 Jun 2020 18:51:32: 8000000 INFO @ Sun, 21 Jun 2020 18:51:38: 13000000 INFO @ Sun, 21 Jun 2020 18:51:38: 17000000 INFO @ Sun, 21 Jun 2020 18:51:40: 9000000 INFO @ Sun, 21 Jun 2020 18:51:45: 14000000 INFO @ Sun, 21 Jun 2020 18:51:46: 18000000 INFO @ Sun, 21 Jun 2020 18:51:48: 10000000 INFO @ Sun, 21 Jun 2020 18:51:53: 15000000 INFO @ Sun, 21 Jun 2020 18:51:54: 19000000 INFO @ Sun, 21 Jun 2020 18:51:56: 11000000 INFO @ Sun, 21 Jun 2020 18:52:00: 16000000 INFO @ Sun, 21 Jun 2020 18:52:02: 20000000 INFO @ Sun, 21 Jun 2020 18:52:04: 12000000 INFO @ Sun, 21 Jun 2020 18:52:07: 17000000 INFO @ Sun, 21 Jun 2020 18:52:10: 21000000 INFO @ Sun, 21 Jun 2020 18:52:12: 13000000 INFO @ Sun, 21 Jun 2020 18:52:15: 18000000 INFO @ Sun, 21 Jun 2020 18:52:18: 22000000 INFO @ Sun, 21 Jun 2020 18:52:20: 14000000 INFO @ Sun, 21 Jun 2020 18:52:22: 19000000 INFO @ Sun, 21 Jun 2020 18:52:26: 23000000 INFO @ Sun, 21 Jun 2020 18:52:28: 15000000 INFO @ Sun, 21 Jun 2020 18:52:30: 20000000 INFO @ Sun, 21 Jun 2020 18:52:34: 24000000 INFO @ Sun, 21 Jun 2020 18:52:36: 16000000 INFO @ Sun, 21 Jun 2020 18:52:37: 21000000 INFO @ Sun, 21 Jun 2020 18:52:42: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:52:44: 17000000 INFO @ Sun, 21 Jun 2020 18:52:45: 22000000 INFO @ Sun, 21 Jun 2020 18:52:50: 26000000 INFO @ Sun, 21 Jun 2020 18:52:52: 23000000 INFO @ Sun, 21 Jun 2020 18:52:52: 18000000 INFO @ Sun, 21 Jun 2020 18:52:58: 27000000 INFO @ Sun, 21 Jun 2020 18:52:59: 24000000 INFO @ Sun, 21 Jun 2020 18:53:00: 19000000 INFO @ Sun, 21 Jun 2020 18:53:06: 28000000 INFO @ Sun, 21 Jun 2020 18:53:07: 25000000 INFO @ Sun, 21 Jun 2020 18:53:08: 20000000 INFO @ Sun, 21 Jun 2020 18:53:14: 29000000 INFO @ Sun, 21 Jun 2020 18:53:15: 26000000 INFO @ Sun, 21 Jun 2020 18:53:16: 21000000 INFO @ Sun, 21 Jun 2020 18:53:22: 30000000 INFO @ Sun, 21 Jun 2020 18:53:23: 27000000 INFO @ Sun, 21 Jun 2020 18:53:24: 22000000 INFO @ Sun, 21 Jun 2020 18:53:30: 28000000 INFO @ Sun, 21 Jun 2020 18:53:30: 31000000 INFO @ Sun, 21 Jun 2020 18:53:32: 23000000 INFO @ Sun, 21 Jun 2020 18:53:37: 29000000 INFO @ Sun, 21 Jun 2020 18:53:39: 32000000 INFO @ Sun, 21 Jun 2020 18:53:39: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:53:39: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:53:39: #1 total tags in treatment: 32041793 INFO @ Sun, 21 Jun 2020 18:53:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:53:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:53:40: #1 tags after filtering in treatment: 32041792 INFO @ Sun, 21 Jun 2020 18:53:40: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:53:40: #1 finished! INFO @ Sun, 21 Jun 2020 18:53:40: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:53:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:53:40: 24000000 INFO @ Sun, 21 Jun 2020 18:53:42: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 18:53:42: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:53:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:53:45: 30000000 INFO @ Sun, 21 Jun 2020 18:53:48: 25000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:53:52: 31000000 INFO @ Sun, 21 Jun 2020 18:53:56: 26000000 INFO @ Sun, 21 Jun 2020 18:54:00: 32000000 INFO @ Sun, 21 Jun 2020 18:54:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:54:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:54:00: #1 total tags in treatment: 32041793 INFO @ Sun, 21 Jun 2020 18:54:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:54:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:54:01: #1 tags after filtering in treatment: 32041792 INFO @ Sun, 21 Jun 2020 18:54:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:54:01: #1 finished! INFO @ Sun, 21 Jun 2020 18:54:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:54:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:54:03: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 18:54:03: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:54:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:54:04: 27000000 INFO @ Sun, 21 Jun 2020 18:54:11: 28000000 INFO @ Sun, 21 Jun 2020 18:54:19: 29000000 INFO @ Sun, 21 Jun 2020 18:54:26: 30000000 INFO @ Sun, 21 Jun 2020 18:54:33: 31000000 INFO @ Sun, 21 Jun 2020 18:54:40: 32000000 INFO @ Sun, 21 Jun 2020 18:54:41: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:54:41: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:54:41: #1 total tags in treatment: 32041793 INFO @ Sun, 21 Jun 2020 18:54:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:54:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:54:42: #1 tags after filtering in treatment: 32041792 INFO @ Sun, 21 Jun 2020 18:54:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:54:42: #1 finished! INFO @ Sun, 21 Jun 2020 18:54:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:54:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:54:44: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 18:54:44: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:54:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135529/SRX135529.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling