Job ID = 6453664 SRX = SRX135526 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:27:26 prefetch.2.10.7: 1) Downloading 'SRR453251'... 2020-06-21T08:27:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:04:55 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:04:55 prefetch.2.10.7: 1) 'SRR453251' was downloaded successfully Read 127252800 spots for SRR453251/SRR453251.sra Written 127252800 spots for SRR453251/SRR453251.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:33:23 127252800 reads; of these: 127252800 (100.00%) were unpaired; of these: 12070696 (9.49%) aligned 0 times 90160903 (70.85%) aligned exactly 1 time 25021201 (19.66%) aligned >1 times 90.51% overall alignment rate Time searching: 00:33:23 Overall time: 00:33:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 69450085 / 115182104 = 0.6030 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:09:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:09:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:09:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:09:34: 1000000 INFO @ Sun, 21 Jun 2020 19:09:41: 2000000 INFO @ Sun, 21 Jun 2020 19:09:48: 3000000 INFO @ Sun, 21 Jun 2020 19:09:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:09:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:09:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:09:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:10:02: 5000000 INFO @ Sun, 21 Jun 2020 19:10:04: 1000000 INFO @ Sun, 21 Jun 2020 19:10:10: 6000000 INFO @ Sun, 21 Jun 2020 19:10:11: 2000000 INFO @ Sun, 21 Jun 2020 19:10:18: 7000000 INFO @ Sun, 21 Jun 2020 19:10:18: 3000000 INFO @ Sun, 21 Jun 2020 19:10:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:10:25: 8000000 INFO @ Sun, 21 Jun 2020 19:10:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:10:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:10:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:10:32: 5000000 INFO @ Sun, 21 Jun 2020 19:10:33: 9000000 INFO @ Sun, 21 Jun 2020 19:10:34: 1000000 INFO @ Sun, 21 Jun 2020 19:10:39: 6000000 INFO @ Sun, 21 Jun 2020 19:10:41: 10000000 INFO @ Sun, 21 Jun 2020 19:10:41: 2000000 INFO @ Sun, 21 Jun 2020 19:10:46: 7000000 INFO @ Sun, 21 Jun 2020 19:10:48: 3000000 INFO @ Sun, 21 Jun 2020 19:10:49: 11000000 INFO @ Sun, 21 Jun 2020 19:10:53: 8000000 INFO @ Sun, 21 Jun 2020 19:10:55: 4000000 INFO @ Sun, 21 Jun 2020 19:10:56: 12000000 INFO @ Sun, 21 Jun 2020 19:11:00: 9000000 INFO @ Sun, 21 Jun 2020 19:11:02: 5000000 INFO @ Sun, 21 Jun 2020 19:11:04: 13000000 INFO @ Sun, 21 Jun 2020 19:11:07: 10000000 INFO @ Sun, 21 Jun 2020 19:11:09: 6000000 INFO @ Sun, 21 Jun 2020 19:11:12: 14000000 INFO @ Sun, 21 Jun 2020 19:11:14: 11000000 INFO @ Sun, 21 Jun 2020 19:11:16: 7000000 INFO @ Sun, 21 Jun 2020 19:11:19: 15000000 INFO @ Sun, 21 Jun 2020 19:11:21: 12000000 INFO @ Sun, 21 Jun 2020 19:11:22: 8000000 INFO @ Sun, 21 Jun 2020 19:11:26: 16000000 INFO @ Sun, 21 Jun 2020 19:11:27: 13000000 INFO @ Sun, 21 Jun 2020 19:11:29: 9000000 INFO @ Sun, 21 Jun 2020 19:11:34: 17000000 INFO @ Sun, 21 Jun 2020 19:11:34: 14000000 INFO @ Sun, 21 Jun 2020 19:11:36: 10000000 INFO @ Sun, 21 Jun 2020 19:11:41: 15000000 INFO @ Sun, 21 Jun 2020 19:11:42: 18000000 INFO @ Sun, 21 Jun 2020 19:11:43: 11000000 INFO @ Sun, 21 Jun 2020 19:11:48: 16000000 INFO @ Sun, 21 Jun 2020 19:11:49: 12000000 INFO @ Sun, 21 Jun 2020 19:11:50: 19000000 INFO @ Sun, 21 Jun 2020 19:11:54: 17000000 INFO @ Sun, 21 Jun 2020 19:11:56: 13000000 INFO @ Sun, 21 Jun 2020 19:11:58: 20000000 INFO @ Sun, 21 Jun 2020 19:12:01: 18000000 INFO @ Sun, 21 Jun 2020 19:12:02: 14000000 INFO @ Sun, 21 Jun 2020 19:12:05: 21000000 INFO @ Sun, 21 Jun 2020 19:12:08: 19000000 INFO @ Sun, 21 Jun 2020 19:12:09: 15000000 INFO @ Sun, 21 Jun 2020 19:12:12: 22000000 INFO @ Sun, 21 Jun 2020 19:12:14: 20000000 INFO @ Sun, 21 Jun 2020 19:12:16: 16000000 INFO @ Sun, 21 Jun 2020 19:12:20: 23000000 INFO @ Sun, 21 Jun 2020 19:12:21: 21000000 INFO @ Sun, 21 Jun 2020 19:12:22: 17000000 INFO @ Sun, 21 Jun 2020 19:12:27: 24000000 INFO @ Sun, 21 Jun 2020 19:12:27: 22000000 INFO @ Sun, 21 Jun 2020 19:12:29: 18000000 INFO @ Sun, 21 Jun 2020 19:12:34: 25000000 INFO @ Sun, 21 Jun 2020 19:12:34: 23000000 INFO @ Sun, 21 Jun 2020 19:12:35: 19000000 INFO @ Sun, 21 Jun 2020 19:12:41: 24000000 INFO @ Sun, 21 Jun 2020 19:12:41: 26000000 INFO @ Sun, 21 Jun 2020 19:12:42: 20000000 INFO @ Sun, 21 Jun 2020 19:12:47: 25000000 INFO @ Sun, 21 Jun 2020 19:12:48: 27000000 INFO @ Sun, 21 Jun 2020 19:12:48: 21000000 INFO @ Sun, 21 Jun 2020 19:12:54: 26000000 INFO @ Sun, 21 Jun 2020 19:12:55: 22000000 INFO @ Sun, 21 Jun 2020 19:12:55: 28000000 INFO @ Sun, 21 Jun 2020 19:13:00: 27000000 INFO @ Sun, 21 Jun 2020 19:13:01: 23000000 INFO @ Sun, 21 Jun 2020 19:13:02: 29000000 INFO @ Sun, 21 Jun 2020 19:13:07: 28000000 INFO @ Sun, 21 Jun 2020 19:13:08: 24000000 INFO @ Sun, 21 Jun 2020 19:13:09: 30000000 INFO @ Sun, 21 Jun 2020 19:13:13: 29000000 INFO @ Sun, 21 Jun 2020 19:13:14: 25000000 INFO @ Sun, 21 Jun 2020 19:13:17: 31000000 INFO @ Sun, 21 Jun 2020 19:13:20: 30000000 INFO @ Sun, 21 Jun 2020 19:13:21: 26000000 INFO @ Sun, 21 Jun 2020 19:13:24: 32000000 INFO @ Sun, 21 Jun 2020 19:13:27: 31000000 INFO @ Sun, 21 Jun 2020 19:13:28: 27000000 INFO @ Sun, 21 Jun 2020 19:13:31: 33000000 INFO @ Sun, 21 Jun 2020 19:13:33: 32000000 INFO @ Sun, 21 Jun 2020 19:13:34: 28000000 INFO @ Sun, 21 Jun 2020 19:13:38: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:13:40: 33000000 INFO @ Sun, 21 Jun 2020 19:13:41: 29000000 INFO @ Sun, 21 Jun 2020 19:13:45: 35000000 INFO @ Sun, 21 Jun 2020 19:13:47: 34000000 INFO @ Sun, 21 Jun 2020 19:13:48: 30000000 INFO @ Sun, 21 Jun 2020 19:13:52: 36000000 INFO @ Sun, 21 Jun 2020 19:13:54: 35000000 INFO @ Sun, 21 Jun 2020 19:13:55: 31000000 INFO @ Sun, 21 Jun 2020 19:13:59: 37000000 INFO @ Sun, 21 Jun 2020 19:14:01: 36000000 INFO @ Sun, 21 Jun 2020 19:14:02: 32000000 INFO @ Sun, 21 Jun 2020 19:14:07: 38000000 INFO @ Sun, 21 Jun 2020 19:14:08: 37000000 INFO @ Sun, 21 Jun 2020 19:14:09: 33000000 INFO @ Sun, 21 Jun 2020 19:14:14: 39000000 INFO @ Sun, 21 Jun 2020 19:14:15: 38000000 INFO @ Sun, 21 Jun 2020 19:14:15: 34000000 INFO @ Sun, 21 Jun 2020 19:14:21: 40000000 INFO @ Sun, 21 Jun 2020 19:14:22: 39000000 INFO @ Sun, 21 Jun 2020 19:14:22: 35000000 INFO @ Sun, 21 Jun 2020 19:14:28: 41000000 INFO @ Sun, 21 Jun 2020 19:14:28: 40000000 INFO @ Sun, 21 Jun 2020 19:14:29: 36000000 INFO @ Sun, 21 Jun 2020 19:14:35: 42000000 INFO @ Sun, 21 Jun 2020 19:14:35: 41000000 INFO @ Sun, 21 Jun 2020 19:14:36: 37000000 INFO @ Sun, 21 Jun 2020 19:14:42: 42000000 INFO @ Sun, 21 Jun 2020 19:14:42: 43000000 INFO @ Sun, 21 Jun 2020 19:14:43: 38000000 INFO @ Sun, 21 Jun 2020 19:14:49: 43000000 INFO @ Sun, 21 Jun 2020 19:14:49: 44000000 INFO @ Sun, 21 Jun 2020 19:14:49: 39000000 INFO @ Sun, 21 Jun 2020 19:14:55: 44000000 INFO @ Sun, 21 Jun 2020 19:14:56: 40000000 INFO @ Sun, 21 Jun 2020 19:14:57: 45000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:15:02: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:15:02: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:15:02: #1 total tags in treatment: 45732019 INFO @ Sun, 21 Jun 2020 19:15:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:15:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:15:02: 45000000 INFO @ Sun, 21 Jun 2020 19:15:03: 41000000 INFO @ Sun, 21 Jun 2020 19:15:03: #1 tags after filtering in treatment: 45732018 INFO @ Sun, 21 Jun 2020 19:15:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:15:03: #1 finished! INFO @ Sun, 21 Jun 2020 19:15:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:15:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:15:06: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:15:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:15:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:15:07: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:15:07: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:15:07: #1 total tags in treatment: 45732019 INFO @ Sun, 21 Jun 2020 19:15:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:15:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:15:08: #1 tags after filtering in treatment: 45732018 INFO @ Sun, 21 Jun 2020 19:15:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:15:08: #1 finished! INFO @ Sun, 21 Jun 2020 19:15:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:15:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:15:09: 42000000 INFO @ Sun, 21 Jun 2020 19:15:11: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:15:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:15:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:15:14: 43000000 INFO @ Sun, 21 Jun 2020 19:15:20: 44000000 INFO @ Sun, 21 Jun 2020 19:15:26: 45000000 INFO @ Sun, 21 Jun 2020 19:15:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:15:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:15:30: #1 total tags in treatment: 45732019 INFO @ Sun, 21 Jun 2020 19:15:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:15:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:15:31: #1 tags after filtering in treatment: 45732018 INFO @ Sun, 21 Jun 2020 19:15:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:15:31: #1 finished! INFO @ Sun, 21 Jun 2020 19:15:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:15:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:15:34: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:15:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:15:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135526/SRX135526.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling