Job ID = 6453662 SRX = SRX135525 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:34:24 prefetch.2.10.7: 1) Downloading 'SRR453250'... 2020-06-21T08:34:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:52:47 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:52:47 prefetch.2.10.7: 1) 'SRR453250' was downloaded successfully Read 96713808 spots for SRR453250/SRR453250.sra Written 96713808 spots for SRR453250/SRR453250.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:18 96713808 reads; of these: 96713808 (100.00%) were unpaired; of these: 7353507 (7.60%) aligned 0 times 79981462 (82.70%) aligned exactly 1 time 9378839 (9.70%) aligned >1 times 92.40% overall alignment rate Time searching: 00:22:18 Overall time: 00:22:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 46860929 / 89360301 = 0.5244 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:40:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:40:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:40:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:40:39: 1000000 INFO @ Sun, 21 Jun 2020 18:40:45: 2000000 INFO @ Sun, 21 Jun 2020 18:40:50: 3000000 INFO @ Sun, 21 Jun 2020 18:40:56: 4000000 INFO @ Sun, 21 Jun 2020 18:41:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:41:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:41:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:41:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:41:06: 6000000 INFO @ Sun, 21 Jun 2020 18:41:09: 1000000 INFO @ Sun, 21 Jun 2020 18:41:12: 7000000 INFO @ Sun, 21 Jun 2020 18:41:15: 2000000 INFO @ Sun, 21 Jun 2020 18:41:17: 8000000 INFO @ Sun, 21 Jun 2020 18:41:20: 3000000 INFO @ Sun, 21 Jun 2020 18:41:23: 9000000 INFO @ Sun, 21 Jun 2020 18:41:26: 4000000 INFO @ Sun, 21 Jun 2020 18:41:28: 10000000 INFO @ Sun, 21 Jun 2020 18:41:31: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:41:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:41:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:41:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:41:34: 11000000 INFO @ Sun, 21 Jun 2020 18:41:37: 6000000 INFO @ Sun, 21 Jun 2020 18:41:39: 1000000 INFO @ Sun, 21 Jun 2020 18:41:39: 12000000 INFO @ Sun, 21 Jun 2020 18:41:42: 7000000 INFO @ Sun, 21 Jun 2020 18:41:44: 2000000 INFO @ Sun, 21 Jun 2020 18:41:45: 13000000 INFO @ Sun, 21 Jun 2020 18:41:47: 8000000 INFO @ Sun, 21 Jun 2020 18:41:49: 3000000 INFO @ Sun, 21 Jun 2020 18:41:51: 14000000 INFO @ Sun, 21 Jun 2020 18:41:53: 9000000 INFO @ Sun, 21 Jun 2020 18:41:55: 4000000 INFO @ Sun, 21 Jun 2020 18:41:56: 15000000 INFO @ Sun, 21 Jun 2020 18:41:58: 10000000 INFO @ Sun, 21 Jun 2020 18:42:00: 5000000 INFO @ Sun, 21 Jun 2020 18:42:02: 16000000 INFO @ Sun, 21 Jun 2020 18:42:04: 11000000 INFO @ Sun, 21 Jun 2020 18:42:05: 6000000 INFO @ Sun, 21 Jun 2020 18:42:08: 17000000 INFO @ Sun, 21 Jun 2020 18:42:09: 12000000 INFO @ Sun, 21 Jun 2020 18:42:11: 7000000 INFO @ Sun, 21 Jun 2020 18:42:13: 18000000 INFO @ Sun, 21 Jun 2020 18:42:14: 13000000 INFO @ Sun, 21 Jun 2020 18:42:16: 8000000 INFO @ Sun, 21 Jun 2020 18:42:19: 19000000 INFO @ Sun, 21 Jun 2020 18:42:20: 14000000 INFO @ Sun, 21 Jun 2020 18:42:21: 9000000 INFO @ Sun, 21 Jun 2020 18:42:24: 20000000 INFO @ Sun, 21 Jun 2020 18:42:25: 15000000 INFO @ Sun, 21 Jun 2020 18:42:27: 10000000 INFO @ Sun, 21 Jun 2020 18:42:30: 21000000 INFO @ Sun, 21 Jun 2020 18:42:31: 16000000 INFO @ Sun, 21 Jun 2020 18:42:32: 11000000 INFO @ Sun, 21 Jun 2020 18:42:36: 22000000 INFO @ Sun, 21 Jun 2020 18:42:37: 17000000 INFO @ Sun, 21 Jun 2020 18:42:37: 12000000 INFO @ Sun, 21 Jun 2020 18:42:41: 23000000 INFO @ Sun, 21 Jun 2020 18:42:42: 18000000 INFO @ Sun, 21 Jun 2020 18:42:43: 13000000 INFO @ Sun, 21 Jun 2020 18:42:47: 24000000 INFO @ Sun, 21 Jun 2020 18:42:48: 19000000 INFO @ Sun, 21 Jun 2020 18:42:48: 14000000 INFO @ Sun, 21 Jun 2020 18:42:53: 25000000 INFO @ Sun, 21 Jun 2020 18:42:53: 20000000 INFO @ Sun, 21 Jun 2020 18:42:54: 15000000 INFO @ Sun, 21 Jun 2020 18:42:59: 26000000 INFO @ Sun, 21 Jun 2020 18:42:59: 21000000 INFO @ Sun, 21 Jun 2020 18:42:59: 16000000 INFO @ Sun, 21 Jun 2020 18:43:04: 22000000 INFO @ Sun, 21 Jun 2020 18:43:04: 27000000 INFO @ Sun, 21 Jun 2020 18:43:05: 17000000 INFO @ Sun, 21 Jun 2020 18:43:10: 23000000 INFO @ Sun, 21 Jun 2020 18:43:10: 28000000 INFO @ Sun, 21 Jun 2020 18:43:10: 18000000 INFO @ Sun, 21 Jun 2020 18:43:15: 24000000 INFO @ Sun, 21 Jun 2020 18:43:15: 19000000 INFO @ Sun, 21 Jun 2020 18:43:16: 29000000 INFO @ Sun, 21 Jun 2020 18:43:21: 25000000 INFO @ Sun, 21 Jun 2020 18:43:21: 20000000 INFO @ Sun, 21 Jun 2020 18:43:21: 30000000 INFO @ Sun, 21 Jun 2020 18:43:26: 26000000 INFO @ Sun, 21 Jun 2020 18:43:26: 21000000 INFO @ Sun, 21 Jun 2020 18:43:27: 31000000 INFO @ Sun, 21 Jun 2020 18:43:31: 27000000 INFO @ Sun, 21 Jun 2020 18:43:31: 22000000 INFO @ Sun, 21 Jun 2020 18:43:32: 32000000 INFO @ Sun, 21 Jun 2020 18:43:37: 23000000 INFO @ Sun, 21 Jun 2020 18:43:37: 28000000 INFO @ Sun, 21 Jun 2020 18:43:38: 33000000 INFO @ Sun, 21 Jun 2020 18:43:42: 24000000 INFO @ Sun, 21 Jun 2020 18:43:42: 29000000 INFO @ Sun, 21 Jun 2020 18:43:44: 34000000 INFO @ Sun, 21 Jun 2020 18:43:48: 25000000 INFO @ Sun, 21 Jun 2020 18:43:48: 30000000 INFO @ Sun, 21 Jun 2020 18:43:49: 35000000 INFO @ Sun, 21 Jun 2020 18:43:53: 26000000 INFO @ Sun, 21 Jun 2020 18:43:54: 31000000 INFO @ Sun, 21 Jun 2020 18:43:55: 36000000 INFO @ Sun, 21 Jun 2020 18:43:59: 27000000 INFO @ Sun, 21 Jun 2020 18:43:59: 32000000 INFO @ Sun, 21 Jun 2020 18:44:01: 37000000 INFO @ Sun, 21 Jun 2020 18:44:04: 28000000 INFO @ Sun, 21 Jun 2020 18:44:05: 33000000 INFO @ Sun, 21 Jun 2020 18:44:06: 38000000 INFO @ Sun, 21 Jun 2020 18:44:10: 29000000 INFO @ Sun, 21 Jun 2020 18:44:10: 34000000 INFO @ Sun, 21 Jun 2020 18:44:12: 39000000 INFO @ Sun, 21 Jun 2020 18:44:16: 30000000 INFO @ Sun, 21 Jun 2020 18:44:16: 35000000 INFO @ Sun, 21 Jun 2020 18:44:17: 40000000 INFO @ Sun, 21 Jun 2020 18:44:21: 31000000 INFO @ Sun, 21 Jun 2020 18:44:22: 36000000 INFO @ Sun, 21 Jun 2020 18:44:23: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:44:27: 32000000 INFO @ Sun, 21 Jun 2020 18:44:28: 37000000 INFO @ Sun, 21 Jun 2020 18:44:29: 42000000 INFO @ Sun, 21 Jun 2020 18:44:32: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:44:32: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:44:32: #1 total tags in treatment: 42499372 INFO @ Sun, 21 Jun 2020 18:44:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:44:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:44:33: 33000000 INFO @ Sun, 21 Jun 2020 18:44:33: #1 tags after filtering in treatment: 42499368 INFO @ Sun, 21 Jun 2020 18:44:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:44:33: #1 finished! INFO @ Sun, 21 Jun 2020 18:44:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:44:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:44:33: 38000000 INFO @ Sun, 21 Jun 2020 18:44:35: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:44:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:44:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:44:38: 34000000 INFO @ Sun, 21 Jun 2020 18:44:39: 39000000 INFO @ Sun, 21 Jun 2020 18:44:44: 35000000 INFO @ Sun, 21 Jun 2020 18:44:44: 40000000 INFO @ Sun, 21 Jun 2020 18:44:49: 41000000 INFO @ Sun, 21 Jun 2020 18:44:50: 36000000 INFO @ Sun, 21 Jun 2020 18:44:55: 42000000 INFO @ Sun, 21 Jun 2020 18:44:55: 37000000 INFO @ Sun, 21 Jun 2020 18:44:58: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:44:58: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:44:58: #1 total tags in treatment: 42499372 INFO @ Sun, 21 Jun 2020 18:44:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:44:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:44:59: #1 tags after filtering in treatment: 42499368 INFO @ Sun, 21 Jun 2020 18:44:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:44:59: #1 finished! INFO @ Sun, 21 Jun 2020 18:44:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:44:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:45:01: 38000000 INFO @ Sun, 21 Jun 2020 18:45:02: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:45:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:45:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:45:06: 39000000 INFO @ Sun, 21 Jun 2020 18:45:12: 40000000 INFO @ Sun, 21 Jun 2020 18:45:17: 41000000 INFO @ Sun, 21 Jun 2020 18:45:23: 42000000 INFO @ Sun, 21 Jun 2020 18:45:26: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:45:26: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:45:26: #1 total tags in treatment: 42499372 INFO @ Sun, 21 Jun 2020 18:45:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:45:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:45:27: #1 tags after filtering in treatment: 42499368 INFO @ Sun, 21 Jun 2020 18:45:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:45:27: #1 finished! INFO @ Sun, 21 Jun 2020 18:45:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:45:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:45:30: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:45:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:45:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135525/SRX135525.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。