Job ID = 6453661 SRX = SRX135524 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:37:40 prefetch.2.10.7: 1) Downloading 'SRR453249'... 2020-06-21T08:37:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:58:05 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:58:05 prefetch.2.10.7: 1) 'SRR453249' was downloaded successfully Read 115256763 spots for SRR453249/SRR453249.sra Written 115256763 spots for SRR453249/SRR453249.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:44 115256763 reads; of these: 115256763 (100.00%) were unpaired; of these: 9671738 (8.39%) aligned 0 times 89372698 (77.54%) aligned exactly 1 time 16212327 (14.07%) aligned >1 times 91.61% overall alignment rate Time searching: 00:27:44 Overall time: 00:27:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 67087923 / 105585025 = 0.6354 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:55:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:55:43: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:55:43: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:55:50: 1000000 INFO @ Sun, 21 Jun 2020 18:55:56: 2000000 INFO @ Sun, 21 Jun 2020 18:56:03: 3000000 INFO @ Sun, 21 Jun 2020 18:56:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:56:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:56:13: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:56:13: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:56:17: 5000000 INFO @ Sun, 21 Jun 2020 18:56:20: 1000000 INFO @ Sun, 21 Jun 2020 18:56:24: 6000000 INFO @ Sun, 21 Jun 2020 18:56:27: 2000000 INFO @ Sun, 21 Jun 2020 18:56:31: 7000000 INFO @ Sun, 21 Jun 2020 18:56:34: 3000000 INFO @ Sun, 21 Jun 2020 18:56:38: 8000000 BedGraph に変換中... INFO @ Sun, 21 Jun 2020 18:56:41: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:56:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:56:43: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:56:43: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:56:44: 9000000 INFO @ Sun, 21 Jun 2020 18:56:48: 5000000 INFO @ Sun, 21 Jun 2020 18:56:50: 1000000 INFO @ Sun, 21 Jun 2020 18:56:51: 10000000 INFO @ Sun, 21 Jun 2020 18:56:55: 6000000 INFO @ Sun, 21 Jun 2020 18:56:57: 2000000 INFO @ Sun, 21 Jun 2020 18:56:58: 11000000 INFO @ Sun, 21 Jun 2020 18:57:02: 7000000 INFO @ Sun, 21 Jun 2020 18:57:04: 3000000 INFO @ Sun, 21 Jun 2020 18:57:06: 12000000 INFO @ Sun, 21 Jun 2020 18:57:09: 8000000 INFO @ Sun, 21 Jun 2020 18:57:11: 4000000 INFO @ Sun, 21 Jun 2020 18:57:13: 13000000 INFO @ Sun, 21 Jun 2020 18:57:16: 9000000 INFO @ Sun, 21 Jun 2020 18:57:18: 5000000 INFO @ Sun, 21 Jun 2020 18:57:20: 14000000 INFO @ Sun, 21 Jun 2020 18:57:23: 10000000 INFO @ Sun, 21 Jun 2020 18:57:25: 6000000 INFO @ Sun, 21 Jun 2020 18:57:27: 15000000 INFO @ Sun, 21 Jun 2020 18:57:30: 11000000 INFO @ Sun, 21 Jun 2020 18:57:32: 7000000 INFO @ Sun, 21 Jun 2020 18:57:34: 16000000 INFO @ Sun, 21 Jun 2020 18:57:37: 12000000 INFO @ Sun, 21 Jun 2020 18:57:40: 8000000 INFO @ Sun, 21 Jun 2020 18:57:41: 17000000 INFO @ Sun, 21 Jun 2020 18:57:44: 13000000 INFO @ Sun, 21 Jun 2020 18:57:47: 9000000 INFO @ Sun, 21 Jun 2020 18:57:48: 18000000 INFO @ Sun, 21 Jun 2020 18:57:52: 14000000 INFO @ Sun, 21 Jun 2020 18:57:54: 10000000 INFO @ Sun, 21 Jun 2020 18:57:55: 19000000 INFO @ Sun, 21 Jun 2020 18:58:00: 15000000 INFO @ Sun, 21 Jun 2020 18:58:01: 11000000 INFO @ Sun, 21 Jun 2020 18:58:02: 20000000 INFO @ Sun, 21 Jun 2020 18:58:08: 12000000 INFO @ Sun, 21 Jun 2020 18:58:08: 16000000 INFO @ Sun, 21 Jun 2020 18:58:10: 21000000 INFO @ Sun, 21 Jun 2020 18:58:16: 13000000 INFO @ Sun, 21 Jun 2020 18:58:16: 17000000 INFO @ Sun, 21 Jun 2020 18:58:17: 22000000 INFO @ Sun, 21 Jun 2020 18:58:23: 14000000 INFO @ Sun, 21 Jun 2020 18:58:24: 23000000 INFO @ Sun, 21 Jun 2020 18:58:25: 18000000 INFO @ Sun, 21 Jun 2020 18:58:30: 15000000 INFO @ Sun, 21 Jun 2020 18:58:31: 24000000 INFO @ Sun, 21 Jun 2020 18:58:32: 19000000 INFO @ Sun, 21 Jun 2020 18:58:37: 16000000 INFO @ Sun, 21 Jun 2020 18:58:39: 25000000 INFO @ Sun, 21 Jun 2020 18:58:39: 20000000 INFO @ Sun, 21 Jun 2020 18:58:44: 17000000 INFO @ Sun, 21 Jun 2020 18:58:46: 26000000 INFO @ Sun, 21 Jun 2020 18:58:46: 21000000 INFO @ Sun, 21 Jun 2020 18:58:52: 18000000 INFO @ Sun, 21 Jun 2020 18:58:53: 27000000 INFO @ Sun, 21 Jun 2020 18:58:53: 22000000 INFO @ Sun, 21 Jun 2020 18:58:59: 19000000 INFO @ Sun, 21 Jun 2020 18:59:00: 28000000 INFO @ Sun, 21 Jun 2020 18:59:01: 23000000 INFO @ Sun, 21 Jun 2020 18:59:06: 20000000 INFO @ Sun, 21 Jun 2020 18:59:07: 29000000 INFO @ Sun, 21 Jun 2020 18:59:08: 24000000 INFO @ Sun, 21 Jun 2020 18:59:13: 21000000 INFO @ Sun, 21 Jun 2020 18:59:14: 30000000 INFO @ Sun, 21 Jun 2020 18:59:15: 25000000 INFO @ Sun, 21 Jun 2020 18:59:21: 22000000 INFO @ Sun, 21 Jun 2020 18:59:22: 26000000 INFO @ Sun, 21 Jun 2020 18:59:22: 31000000 INFO @ Sun, 21 Jun 2020 18:59:28: 23000000 INFO @ Sun, 21 Jun 2020 18:59:29: 27000000 INFO @ Sun, 21 Jun 2020 18:59:30: 32000000 INFO @ Sun, 21 Jun 2020 18:59:35: 24000000 INFO @ Sun, 21 Jun 2020 18:59:36: 28000000 INFO @ Sun, 21 Jun 2020 18:59:37: 33000000 INFO @ Sun, 21 Jun 2020 18:59:42: 25000000 INFO @ Sun, 21 Jun 2020 18:59:43: 29000000 INFO @ Sun, 21 Jun 2020 18:59:44: 34000000 INFO @ Sun, 21 Jun 2020 18:59:49: 26000000 INFO @ Sun, 21 Jun 2020 18:59:50: 30000000 INFO @ Sun, 21 Jun 2020 18:59:51: 35000000 INFO @ Sun, 21 Jun 2020 18:59:56: 27000000 INFO @ Sun, 21 Jun 2020 18:59:57: 31000000 INFO @ Sun, 21 Jun 2020 18:59:58: 36000000 INFO @ Sun, 21 Jun 2020 19:00:03: 28000000 INFO @ Sun, 21 Jun 2020 19:00:05: 37000000 INFO @ Sun, 21 Jun 2020 19:00:05: 32000000 INFO @ Sun, 21 Jun 2020 19:00:10: 29000000 INFO @ Sun, 21 Jun 2020 19:00:12: 38000000 INFO @ Sun, 21 Jun 2020 19:00:12: 33000000 INFO @ Sun, 21 Jun 2020 19:00:16: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:00:16: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:00:16: #1 total tags in treatment: 38497102 INFO @ Sun, 21 Jun 2020 19:00:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:00:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:00:17: #1 tags after filtering in treatment: 38497102 INFO @ Sun, 21 Jun 2020 19:00:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:00:17: #1 finished! INFO @ Sun, 21 Jun 2020 19:00:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:00:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:00:18: 30000000 INFO @ Sun, 21 Jun 2020 19:00:19: 34000000 INFO @ Sun, 21 Jun 2020 19:00:20: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 19:00:20: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:00:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:00:26: 31000000 INFO @ Sun, 21 Jun 2020 19:00:26: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:00:33: 36000000 INFO @ Sun, 21 Jun 2020 19:00:33: 32000000 INFO @ Sun, 21 Jun 2020 19:00:40: 37000000 INFO @ Sun, 21 Jun 2020 19:00:40: 33000000 INFO @ Sun, 21 Jun 2020 19:00:47: 34000000 INFO @ Sun, 21 Jun 2020 19:00:47: 38000000 INFO @ Sun, 21 Jun 2020 19:00:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:00:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:00:51: #1 total tags in treatment: 38497102 INFO @ Sun, 21 Jun 2020 19:00:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:00:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:00:52: #1 tags after filtering in treatment: 38497102 INFO @ Sun, 21 Jun 2020 19:00:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:00:52: #1 finished! INFO @ Sun, 21 Jun 2020 19:00:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:00:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:00:54: 35000000 INFO @ Sun, 21 Jun 2020 19:00:55: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 19:00:55: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:00:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:01:01: 36000000 INFO @ Sun, 21 Jun 2020 19:01:07: 37000000 INFO @ Sun, 21 Jun 2020 19:01:15: 38000000 INFO @ Sun, 21 Jun 2020 19:01:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:01:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:01:19: #1 total tags in treatment: 38497102 INFO @ Sun, 21 Jun 2020 19:01:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:01:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:01:20: #1 tags after filtering in treatment: 38497102 INFO @ Sun, 21 Jun 2020 19:01:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:01:20: #1 finished! INFO @ Sun, 21 Jun 2020 19:01:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:01:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:01:22: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 19:01:22: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:01:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135524/SRX135524.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。