Job ID = 6453660 SRX = SRX135523 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:34:09 prefetch.2.10.7: 1) Downloading 'SRR453248'... 2020-06-21T08:34:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:47:51 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:47:51 prefetch.2.10.7: 1) 'SRR453248' was downloaded successfully Read 105154728 spots for SRR453248/SRR453248.sra Written 105154728 spots for SRR453248/SRR453248.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:52 105154728 reads; of these: 105154728 (100.00%) were unpaired; of these: 8184144 (7.78%) aligned 0 times 63211495 (60.11%) aligned exactly 1 time 33759089 (32.10%) aligned >1 times 92.22% overall alignment rate Time searching: 00:31:52 Overall time: 00:31:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 50251766 / 96970584 = 0.5182 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:45:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:45:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:45:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:45:30: 1000000 INFO @ Sun, 21 Jun 2020 18:45:36: 2000000 INFO @ Sun, 21 Jun 2020 18:45:42: 3000000 INFO @ Sun, 21 Jun 2020 18:45:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:45:53: 5000000 INFO @ Sun, 21 Jun 2020 18:45:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:45:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:45:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:45:59: 6000000 INFO @ Sun, 21 Jun 2020 18:46:00: 1000000 INFO @ Sun, 21 Jun 2020 18:46:06: 7000000 INFO @ Sun, 21 Jun 2020 18:46:07: 2000000 INFO @ Sun, 21 Jun 2020 18:46:12: 8000000 INFO @ Sun, 21 Jun 2020 18:46:13: 3000000 INFO @ Sun, 21 Jun 2020 18:46:19: 9000000 INFO @ Sun, 21 Jun 2020 18:46:20: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:46:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:46:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:46:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:46:25: 10000000 INFO @ Sun, 21 Jun 2020 18:46:27: 5000000 INFO @ Sun, 21 Jun 2020 18:46:31: 1000000 INFO @ Sun, 21 Jun 2020 18:46:32: 11000000 INFO @ Sun, 21 Jun 2020 18:46:33: 6000000 INFO @ Sun, 21 Jun 2020 18:46:37: 2000000 INFO @ Sun, 21 Jun 2020 18:46:38: 12000000 INFO @ Sun, 21 Jun 2020 18:46:39: 7000000 INFO @ Sun, 21 Jun 2020 18:46:44: 3000000 INFO @ Sun, 21 Jun 2020 18:46:45: 13000000 INFO @ Sun, 21 Jun 2020 18:46:46: 8000000 INFO @ Sun, 21 Jun 2020 18:46:51: 4000000 INFO @ Sun, 21 Jun 2020 18:46:52: 14000000 INFO @ Sun, 21 Jun 2020 18:46:52: 9000000 INFO @ Sun, 21 Jun 2020 18:46:57: 5000000 INFO @ Sun, 21 Jun 2020 18:46:58: 15000000 INFO @ Sun, 21 Jun 2020 18:46:59: 10000000 INFO @ Sun, 21 Jun 2020 18:47:04: 6000000 INFO @ Sun, 21 Jun 2020 18:47:05: 16000000 INFO @ Sun, 21 Jun 2020 18:47:05: 11000000 INFO @ Sun, 21 Jun 2020 18:47:10: 7000000 INFO @ Sun, 21 Jun 2020 18:47:11: 17000000 INFO @ Sun, 21 Jun 2020 18:47:12: 12000000 INFO @ Sun, 21 Jun 2020 18:47:17: 8000000 INFO @ Sun, 21 Jun 2020 18:47:18: 18000000 INFO @ Sun, 21 Jun 2020 18:47:18: 13000000 INFO @ Sun, 21 Jun 2020 18:47:23: 9000000 INFO @ Sun, 21 Jun 2020 18:47:25: 19000000 INFO @ Sun, 21 Jun 2020 18:47:25: 14000000 INFO @ Sun, 21 Jun 2020 18:47:29: 10000000 INFO @ Sun, 21 Jun 2020 18:47:31: 20000000 INFO @ Sun, 21 Jun 2020 18:47:31: 15000000 INFO @ Sun, 21 Jun 2020 18:47:36: 11000000 INFO @ Sun, 21 Jun 2020 18:47:38: 21000000 INFO @ Sun, 21 Jun 2020 18:47:38: 16000000 INFO @ Sun, 21 Jun 2020 18:47:42: 12000000 INFO @ Sun, 21 Jun 2020 18:47:44: 22000000 INFO @ Sun, 21 Jun 2020 18:47:44: 17000000 INFO @ Sun, 21 Jun 2020 18:47:49: 13000000 INFO @ Sun, 21 Jun 2020 18:47:51: 23000000 INFO @ Sun, 21 Jun 2020 18:47:51: 18000000 INFO @ Sun, 21 Jun 2020 18:47:55: 14000000 INFO @ Sun, 21 Jun 2020 18:47:57: 24000000 INFO @ Sun, 21 Jun 2020 18:47:57: 19000000 INFO @ Sun, 21 Jun 2020 18:48:02: 15000000 INFO @ Sun, 21 Jun 2020 18:48:04: 25000000 INFO @ Sun, 21 Jun 2020 18:48:04: 20000000 INFO @ Sun, 21 Jun 2020 18:48:08: 16000000 INFO @ Sun, 21 Jun 2020 18:48:10: 26000000 INFO @ Sun, 21 Jun 2020 18:48:11: 21000000 INFO @ Sun, 21 Jun 2020 18:48:15: 17000000 INFO @ Sun, 21 Jun 2020 18:48:17: 27000000 INFO @ Sun, 21 Jun 2020 18:48:17: 22000000 INFO @ Sun, 21 Jun 2020 18:48:21: 18000000 INFO @ Sun, 21 Jun 2020 18:48:24: 28000000 INFO @ Sun, 21 Jun 2020 18:48:24: 23000000 INFO @ Sun, 21 Jun 2020 18:48:28: 19000000 INFO @ Sun, 21 Jun 2020 18:48:30: 29000000 INFO @ Sun, 21 Jun 2020 18:48:30: 24000000 INFO @ Sun, 21 Jun 2020 18:48:35: 20000000 INFO @ Sun, 21 Jun 2020 18:48:37: 30000000 INFO @ Sun, 21 Jun 2020 18:48:37: 25000000 INFO @ Sun, 21 Jun 2020 18:48:41: 21000000 INFO @ Sun, 21 Jun 2020 18:48:43: 31000000 INFO @ Sun, 21 Jun 2020 18:48:43: 26000000 INFO @ Sun, 21 Jun 2020 18:48:48: 22000000 INFO @ Sun, 21 Jun 2020 18:48:50: 32000000 INFO @ Sun, 21 Jun 2020 18:48:50: 27000000 INFO @ Sun, 21 Jun 2020 18:48:55: 23000000 INFO @ Sun, 21 Jun 2020 18:48:56: 33000000 INFO @ Sun, 21 Jun 2020 18:48:56: 28000000 INFO @ Sun, 21 Jun 2020 18:49:01: 24000000 INFO @ Sun, 21 Jun 2020 18:49:03: 34000000 INFO @ Sun, 21 Jun 2020 18:49:03: 29000000 INFO @ Sun, 21 Jun 2020 18:49:08: 25000000 INFO @ Sun, 21 Jun 2020 18:49:10: 30000000 INFO @ Sun, 21 Jun 2020 18:49:10: 35000000 INFO @ Sun, 21 Jun 2020 18:49:15: 26000000 INFO @ Sun, 21 Jun 2020 18:49:16: 31000000 INFO @ Sun, 21 Jun 2020 18:49:16: 36000000 INFO @ Sun, 21 Jun 2020 18:49:22: 27000000 INFO @ Sun, 21 Jun 2020 18:49:23: 32000000 INFO @ Sun, 21 Jun 2020 18:49:23: 37000000 INFO @ Sun, 21 Jun 2020 18:49:29: 28000000 INFO @ Sun, 21 Jun 2020 18:49:30: 33000000 INFO @ Sun, 21 Jun 2020 18:49:30: 38000000 INFO @ Sun, 21 Jun 2020 18:49:35: 29000000 INFO @ Sun, 21 Jun 2020 18:49:37: 34000000 INFO @ Sun, 21 Jun 2020 18:49:37: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:49:42: 30000000 INFO @ Sun, 21 Jun 2020 18:49:44: 40000000 INFO @ Sun, 21 Jun 2020 18:49:44: 35000000 INFO @ Sun, 21 Jun 2020 18:49:49: 31000000 INFO @ Sun, 21 Jun 2020 18:49:51: 41000000 INFO @ Sun, 21 Jun 2020 18:49:51: 36000000 INFO @ Sun, 21 Jun 2020 18:49:56: 32000000 INFO @ Sun, 21 Jun 2020 18:49:58: 42000000 INFO @ Sun, 21 Jun 2020 18:49:58: 37000000 INFO @ Sun, 21 Jun 2020 18:50:03: 33000000 INFO @ Sun, 21 Jun 2020 18:50:04: 43000000 INFO @ Sun, 21 Jun 2020 18:50:06: 38000000 INFO @ Sun, 21 Jun 2020 18:50:10: 34000000 INFO @ Sun, 21 Jun 2020 18:50:11: 44000000 INFO @ Sun, 21 Jun 2020 18:50:13: 39000000 INFO @ Sun, 21 Jun 2020 18:50:17: 35000000 INFO @ Sun, 21 Jun 2020 18:50:18: 45000000 INFO @ Sun, 21 Jun 2020 18:50:21: 40000000 INFO @ Sun, 21 Jun 2020 18:50:23: 36000000 INFO @ Sun, 21 Jun 2020 18:50:25: 46000000 INFO @ Sun, 21 Jun 2020 18:50:28: 41000000 INFO @ Sun, 21 Jun 2020 18:50:30: 37000000 INFO @ Sun, 21 Jun 2020 18:50:31: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:50:31: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:50:31: #1 total tags in treatment: 46718818 INFO @ Sun, 21 Jun 2020 18:50:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:50:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:50:32: #1 tags after filtering in treatment: 46718818 INFO @ Sun, 21 Jun 2020 18:50:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:50:32: #1 finished! INFO @ Sun, 21 Jun 2020 18:50:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:50:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:50:35: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:50:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:50:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:50:35: 42000000 INFO @ Sun, 21 Jun 2020 18:50:37: 38000000 INFO @ Sun, 21 Jun 2020 18:50:42: 43000000 INFO @ Sun, 21 Jun 2020 18:50:44: 39000000 INFO @ Sun, 21 Jun 2020 18:50:49: 44000000 INFO @ Sun, 21 Jun 2020 18:50:50: 40000000 INFO @ Sun, 21 Jun 2020 18:50:56: 45000000 INFO @ Sun, 21 Jun 2020 18:50:57: 41000000 INFO @ Sun, 21 Jun 2020 18:51:03: 42000000 INFO @ Sun, 21 Jun 2020 18:51:04: 46000000 INFO @ Sun, 21 Jun 2020 18:51:09: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:51:09: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:51:09: #1 total tags in treatment: 46718818 INFO @ Sun, 21 Jun 2020 18:51:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:51:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:51:10: 43000000 INFO @ Sun, 21 Jun 2020 18:51:10: #1 tags after filtering in treatment: 46718818 INFO @ Sun, 21 Jun 2020 18:51:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:51:10: #1 finished! INFO @ Sun, 21 Jun 2020 18:51:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:51:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:51:13: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:51:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:51:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:51:16: 44000000 INFO @ Sun, 21 Jun 2020 18:51:22: 45000000 INFO @ Sun, 21 Jun 2020 18:51:28: 46000000 INFO @ Sun, 21 Jun 2020 18:51:32: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:51:32: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:51:32: #1 total tags in treatment: 46718818 INFO @ Sun, 21 Jun 2020 18:51:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:51:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:51:33: #1 tags after filtering in treatment: 46718818 INFO @ Sun, 21 Jun 2020 18:51:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:51:33: #1 finished! INFO @ Sun, 21 Jun 2020 18:51:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:51:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:51:36: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:51:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:51:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135523/SRX135523.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling