Job ID = 6453659 SRX = SRX135522 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:39:48 prefetch.2.10.7: 1) Downloading 'SRR453247'... 2020-06-21T08:39:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:04:08 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:04:08 prefetch.2.10.7: 1) 'SRR453247' was downloaded successfully Read 81219748 spots for SRR453247/SRR453247.sra Written 81219748 spots for SRR453247/SRR453247.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:46 81219748 reads; of these: 81219748 (100.00%) were unpaired; of these: 5801457 (7.14%) aligned 0 times 60958829 (75.05%) aligned exactly 1 time 14459462 (17.80%) aligned >1 times 92.86% overall alignment rate Time searching: 00:21:46 Overall time: 00:21:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 40557853 / 75418291 = 0.5378 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:45:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:45:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:45:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:45:56: 1000000 INFO @ Sun, 21 Jun 2020 18:46:01: 2000000 INFO @ Sun, 21 Jun 2020 18:46:07: 3000000 INFO @ Sun, 21 Jun 2020 18:46:12: 4000000 INFO @ Sun, 21 Jun 2020 18:46:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:46:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:46:20: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:46:20: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:46:23: 6000000 INFO @ Sun, 21 Jun 2020 18:46:26: 1000000 INFO @ Sun, 21 Jun 2020 18:46:29: 7000000 INFO @ Sun, 21 Jun 2020 18:46:33: 2000000 INFO @ Sun, 21 Jun 2020 18:46:35: 8000000 INFO @ Sun, 21 Jun 2020 18:46:38: 3000000 INFO @ Sun, 21 Jun 2020 18:46:41: 9000000 INFO @ Sun, 21 Jun 2020 18:46:44: 4000000 INFO @ Sun, 21 Jun 2020 18:46:48: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:46:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:46:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:46:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:46:51: 5000000 INFO @ Sun, 21 Jun 2020 18:46:54: 11000000 INFO @ Sun, 21 Jun 2020 18:46:57: 1000000 INFO @ Sun, 21 Jun 2020 18:46:57: 6000000 INFO @ Sun, 21 Jun 2020 18:47:01: 12000000 INFO @ Sun, 21 Jun 2020 18:47:04: 7000000 INFO @ Sun, 21 Jun 2020 18:47:04: 2000000 INFO @ Sun, 21 Jun 2020 18:47:07: 13000000 INFO @ Sun, 21 Jun 2020 18:47:11: 8000000 INFO @ Sun, 21 Jun 2020 18:47:11: 3000000 INFO @ Sun, 21 Jun 2020 18:47:14: 14000000 INFO @ Sun, 21 Jun 2020 18:47:17: 9000000 INFO @ Sun, 21 Jun 2020 18:47:18: 4000000 INFO @ Sun, 21 Jun 2020 18:47:21: 15000000 INFO @ Sun, 21 Jun 2020 18:47:24: 10000000 INFO @ Sun, 21 Jun 2020 18:47:25: 5000000 INFO @ Sun, 21 Jun 2020 18:47:28: 16000000 INFO @ Sun, 21 Jun 2020 18:47:31: 11000000 INFO @ Sun, 21 Jun 2020 18:47:32: 6000000 INFO @ Sun, 21 Jun 2020 18:47:35: 17000000 INFO @ Sun, 21 Jun 2020 18:47:38: 12000000 INFO @ Sun, 21 Jun 2020 18:47:40: 7000000 INFO @ Sun, 21 Jun 2020 18:47:42: 18000000 INFO @ Sun, 21 Jun 2020 18:47:45: 13000000 INFO @ Sun, 21 Jun 2020 18:47:48: 8000000 INFO @ Sun, 21 Jun 2020 18:47:49: 19000000 INFO @ Sun, 21 Jun 2020 18:47:51: 14000000 INFO @ Sun, 21 Jun 2020 18:47:55: 9000000 INFO @ Sun, 21 Jun 2020 18:47:56: 20000000 INFO @ Sun, 21 Jun 2020 18:47:58: 15000000 INFO @ Sun, 21 Jun 2020 18:48:03: 21000000 INFO @ Sun, 21 Jun 2020 18:48:03: 10000000 INFO @ Sun, 21 Jun 2020 18:48:05: 16000000 INFO @ Sun, 21 Jun 2020 18:48:10: 22000000 INFO @ Sun, 21 Jun 2020 18:48:11: 11000000 INFO @ Sun, 21 Jun 2020 18:48:12: 17000000 INFO @ Sun, 21 Jun 2020 18:48:17: 23000000 INFO @ Sun, 21 Jun 2020 18:48:18: 18000000 INFO @ Sun, 21 Jun 2020 18:48:18: 12000000 INFO @ Sun, 21 Jun 2020 18:48:24: 24000000 INFO @ Sun, 21 Jun 2020 18:48:25: 19000000 INFO @ Sun, 21 Jun 2020 18:48:26: 13000000 INFO @ Sun, 21 Jun 2020 18:48:31: 25000000 INFO @ Sun, 21 Jun 2020 18:48:32: 20000000 INFO @ Sun, 21 Jun 2020 18:48:33: 14000000 INFO @ Sun, 21 Jun 2020 18:48:37: 26000000 INFO @ Sun, 21 Jun 2020 18:48:39: 21000000 INFO @ Sun, 21 Jun 2020 18:48:41: 15000000 INFO @ Sun, 21 Jun 2020 18:48:44: 27000000 INFO @ Sun, 21 Jun 2020 18:48:46: 22000000 INFO @ Sun, 21 Jun 2020 18:48:48: 16000000 INFO @ Sun, 21 Jun 2020 18:48:51: 28000000 INFO @ Sun, 21 Jun 2020 18:48:53: 23000000 INFO @ Sun, 21 Jun 2020 18:48:55: 17000000 INFO @ Sun, 21 Jun 2020 18:48:58: 29000000 INFO @ Sun, 21 Jun 2020 18:49:00: 24000000 INFO @ Sun, 21 Jun 2020 18:49:02: 18000000 INFO @ Sun, 21 Jun 2020 18:49:05: 30000000 INFO @ Sun, 21 Jun 2020 18:49:06: 25000000 INFO @ Sun, 21 Jun 2020 18:49:09: 19000000 INFO @ Sun, 21 Jun 2020 18:49:12: 31000000 INFO @ Sun, 21 Jun 2020 18:49:13: 26000000 INFO @ Sun, 21 Jun 2020 18:49:16: 20000000 INFO @ Sun, 21 Jun 2020 18:49:18: 32000000 INFO @ Sun, 21 Jun 2020 18:49:20: 27000000 INFO @ Sun, 21 Jun 2020 18:49:23: 21000000 INFO @ Sun, 21 Jun 2020 18:49:25: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:49:27: 28000000 INFO @ Sun, 21 Jun 2020 18:49:30: 22000000 INFO @ Sun, 21 Jun 2020 18:49:32: 34000000 INFO @ Sun, 21 Jun 2020 18:49:34: 29000000 INFO @ Sun, 21 Jun 2020 18:49:37: 23000000 INFO @ Sun, 21 Jun 2020 18:49:38: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:49:38: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:49:38: #1 total tags in treatment: 34860438 INFO @ Sun, 21 Jun 2020 18:49:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:49:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:49:39: #1 tags after filtering in treatment: 34860438 INFO @ Sun, 21 Jun 2020 18:49:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:49:39: #1 finished! INFO @ Sun, 21 Jun 2020 18:49:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:49:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:49:40: 30000000 INFO @ Sun, 21 Jun 2020 18:49:41: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:49:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:49:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:49:44: 24000000 INFO @ Sun, 21 Jun 2020 18:49:47: 31000000 INFO @ Sun, 21 Jun 2020 18:49:52: 25000000 INFO @ Sun, 21 Jun 2020 18:49:53: 32000000 INFO @ Sun, 21 Jun 2020 18:49:58: 26000000 INFO @ Sun, 21 Jun 2020 18:50:00: 33000000 INFO @ Sun, 21 Jun 2020 18:50:05: 27000000 INFO @ Sun, 21 Jun 2020 18:50:07: 34000000 INFO @ Sun, 21 Jun 2020 18:50:12: 28000000 INFO @ Sun, 21 Jun 2020 18:50:13: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:50:13: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:50:13: #1 total tags in treatment: 34860438 INFO @ Sun, 21 Jun 2020 18:50:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:50:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:50:14: #1 tags after filtering in treatment: 34860438 INFO @ Sun, 21 Jun 2020 18:50:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:50:14: #1 finished! INFO @ Sun, 21 Jun 2020 18:50:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:50:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:50:16: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:50:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:50:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:50:19: 29000000 INFO @ Sun, 21 Jun 2020 18:50:26: 30000000 INFO @ Sun, 21 Jun 2020 18:50:32: 31000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:50:39: 32000000 INFO @ Sun, 21 Jun 2020 18:50:45: 33000000 INFO @ Sun, 21 Jun 2020 18:50:52: 34000000 INFO @ Sun, 21 Jun 2020 18:50:58: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:50:58: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:50:58: #1 total tags in treatment: 34860438 INFO @ Sun, 21 Jun 2020 18:50:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:50:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:50:59: #1 tags after filtering in treatment: 34860438 INFO @ Sun, 21 Jun 2020 18:50:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:50:59: #1 finished! INFO @ Sun, 21 Jun 2020 18:50:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:50:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:51:01: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:51:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:51:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135522/SRX135522.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling