Job ID = 6453658 SRX = SRX135521 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:35:54 prefetch.2.10.7: 1) Downloading 'SRR453246'... 2020-06-21T08:35:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:48:00 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:48:00 prefetch.2.10.7: 1) 'SRR453246' was downloaded successfully Read 103392036 spots for SRR453246/SRR453246.sra Written 103392036 spots for SRR453246/SRR453246.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:03 103392036 reads; of these: 103392036 (100.00%) were unpaired; of these: 8760516 (8.47%) aligned 0 times 74281128 (71.84%) aligned exactly 1 time 20350392 (19.68%) aligned >1 times 91.53% overall alignment rate Time searching: 00:26:03 Overall time: 00:26:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 53180511 / 94631520 = 0.5620 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:41:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:41:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:41:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:41:56: 1000000 INFO @ Sun, 21 Jun 2020 18:42:02: 2000000 INFO @ Sun, 21 Jun 2020 18:42:07: 3000000 INFO @ Sun, 21 Jun 2020 18:42:12: 4000000 INFO @ Sun, 21 Jun 2020 18:42:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:42:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:42:20: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:42:20: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:42:23: 6000000 INFO @ Sun, 21 Jun 2020 18:42:26: 1000000 INFO @ Sun, 21 Jun 2020 18:42:29: 7000000 INFO @ Sun, 21 Jun 2020 18:42:32: 2000000 INFO @ Sun, 21 Jun 2020 18:42:35: 8000000 INFO @ Sun, 21 Jun 2020 18:42:38: 3000000 INFO @ Sun, 21 Jun 2020 18:42:41: 9000000 INFO @ Sun, 21 Jun 2020 18:42:45: 4000000 INFO @ Sun, 21 Jun 2020 18:42:46: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:42:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:42:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:42:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:42:51: 5000000 INFO @ Sun, 21 Jun 2020 18:42:52: 11000000 INFO @ Sun, 21 Jun 2020 18:42:56: 1000000 INFO @ Sun, 21 Jun 2020 18:42:58: 6000000 INFO @ Sun, 21 Jun 2020 18:42:59: 12000000 INFO @ Sun, 21 Jun 2020 18:43:02: 2000000 INFO @ Sun, 21 Jun 2020 18:43:04: 7000000 INFO @ Sun, 21 Jun 2020 18:43:05: 13000000 INFO @ Sun, 21 Jun 2020 18:43:08: 3000000 INFO @ Sun, 21 Jun 2020 18:43:11: 14000000 INFO @ Sun, 21 Jun 2020 18:43:11: 8000000 INFO @ Sun, 21 Jun 2020 18:43:14: 4000000 INFO @ Sun, 21 Jun 2020 18:43:16: 15000000 INFO @ Sun, 21 Jun 2020 18:43:18: 9000000 INFO @ Sun, 21 Jun 2020 18:43:20: 5000000 INFO @ Sun, 21 Jun 2020 18:43:22: 16000000 INFO @ Sun, 21 Jun 2020 18:43:25: 10000000 INFO @ Sun, 21 Jun 2020 18:43:26: 6000000 INFO @ Sun, 21 Jun 2020 18:43:28: 17000000 INFO @ Sun, 21 Jun 2020 18:43:31: 11000000 INFO @ Sun, 21 Jun 2020 18:43:32: 7000000 INFO @ Sun, 21 Jun 2020 18:43:34: 18000000 INFO @ Sun, 21 Jun 2020 18:43:38: 8000000 INFO @ Sun, 21 Jun 2020 18:43:38: 12000000 INFO @ Sun, 21 Jun 2020 18:43:40: 19000000 INFO @ Sun, 21 Jun 2020 18:43:44: 9000000 INFO @ Sun, 21 Jun 2020 18:43:44: 13000000 INFO @ Sun, 21 Jun 2020 18:43:46: 20000000 INFO @ Sun, 21 Jun 2020 18:43:50: 10000000 INFO @ Sun, 21 Jun 2020 18:43:51: 14000000 INFO @ Sun, 21 Jun 2020 18:43:52: 21000000 INFO @ Sun, 21 Jun 2020 18:43:56: 11000000 INFO @ Sun, 21 Jun 2020 18:43:58: 15000000 INFO @ Sun, 21 Jun 2020 18:43:58: 22000000 INFO @ Sun, 21 Jun 2020 18:44:02: 12000000 INFO @ Sun, 21 Jun 2020 18:44:04: 23000000 INFO @ Sun, 21 Jun 2020 18:44:04: 16000000 INFO @ Sun, 21 Jun 2020 18:44:07: 13000000 INFO @ Sun, 21 Jun 2020 18:44:09: 24000000 INFO @ Sun, 21 Jun 2020 18:44:11: 17000000 INFO @ Sun, 21 Jun 2020 18:44:13: 14000000 INFO @ Sun, 21 Jun 2020 18:44:15: 25000000 INFO @ Sun, 21 Jun 2020 18:44:18: 18000000 INFO @ Sun, 21 Jun 2020 18:44:19: 15000000 INFO @ Sun, 21 Jun 2020 18:44:21: 26000000 INFO @ Sun, 21 Jun 2020 18:44:25: 19000000 INFO @ Sun, 21 Jun 2020 18:44:25: 16000000 INFO @ Sun, 21 Jun 2020 18:44:27: 27000000 INFO @ Sun, 21 Jun 2020 18:44:31: 17000000 INFO @ Sun, 21 Jun 2020 18:44:32: 20000000 INFO @ Sun, 21 Jun 2020 18:44:33: 28000000 INFO @ Sun, 21 Jun 2020 18:44:37: 18000000 INFO @ Sun, 21 Jun 2020 18:44:39: 29000000 INFO @ Sun, 21 Jun 2020 18:44:40: 21000000 INFO @ Sun, 21 Jun 2020 18:44:43: 19000000 INFO @ Sun, 21 Jun 2020 18:44:45: 30000000 INFO @ Sun, 21 Jun 2020 18:44:47: 22000000 INFO @ Sun, 21 Jun 2020 18:44:49: 20000000 INFO @ Sun, 21 Jun 2020 18:44:51: 31000000 INFO @ Sun, 21 Jun 2020 18:44:55: 23000000 INFO @ Sun, 21 Jun 2020 18:44:55: 21000000 INFO @ Sun, 21 Jun 2020 18:44:56: 32000000 INFO @ Sun, 21 Jun 2020 18:45:01: 22000000 INFO @ Sun, 21 Jun 2020 18:45:01: 24000000 INFO @ Sun, 21 Jun 2020 18:45:02: 33000000 INFO @ Sun, 21 Jun 2020 18:45:07: 23000000 INFO @ Sun, 21 Jun 2020 18:45:08: 25000000 INFO @ Sun, 21 Jun 2020 18:45:09: 34000000 INFO @ Sun, 21 Jun 2020 18:45:13: 24000000 INFO @ Sun, 21 Jun 2020 18:45:15: 35000000 INFO @ Sun, 21 Jun 2020 18:45:15: 26000000 INFO @ Sun, 21 Jun 2020 18:45:19: 25000000 INFO @ Sun, 21 Jun 2020 18:45:21: 36000000 INFO @ Sun, 21 Jun 2020 18:45:22: 27000000 INFO @ Sun, 21 Jun 2020 18:45:25: 26000000 INFO @ Sun, 21 Jun 2020 18:45:27: 37000000 INFO @ Sun, 21 Jun 2020 18:45:29: 28000000 INFO @ Sun, 21 Jun 2020 18:45:31: 27000000 INFO @ Sun, 21 Jun 2020 18:45:33: 38000000 INFO @ Sun, 21 Jun 2020 18:45:35: 29000000 INFO @ Sun, 21 Jun 2020 18:45:37: 28000000 INFO @ Sun, 21 Jun 2020 18:45:39: 39000000 INFO @ Sun, 21 Jun 2020 18:45:42: 30000000 INFO @ Sun, 21 Jun 2020 18:45:43: 29000000 INFO @ Sun, 21 Jun 2020 18:45:45: 40000000 INFO @ Sun, 21 Jun 2020 18:45:48: 31000000 INFO @ Sun, 21 Jun 2020 18:45:49: 30000000 INFO @ Sun, 21 Jun 2020 18:45:51: 41000000 INFO @ Sun, 21 Jun 2020 18:45:54: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:45:54: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:45:54: #1 total tags in treatment: 41451009 INFO @ Sun, 21 Jun 2020 18:45:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:45:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:45:55: 32000000 INFO @ Sun, 21 Jun 2020 18:45:55: 31000000 INFO @ Sun, 21 Jun 2020 18:45:55: #1 tags after filtering in treatment: 41451009 INFO @ Sun, 21 Jun 2020 18:45:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:45:55: #1 finished! INFO @ Sun, 21 Jun 2020 18:45:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:45:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:45:58: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 18:45:58: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:45:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:46:01: 32000000 INFO @ Sun, 21 Jun 2020 18:46:02: 33000000 INFO @ Sun, 21 Jun 2020 18:46:08: 33000000 INFO @ Sun, 21 Jun 2020 18:46:09: 34000000 INFO @ Sun, 21 Jun 2020 18:46:14: 34000000 INFO @ Sun, 21 Jun 2020 18:46:15: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:46:20: 35000000 INFO @ Sun, 21 Jun 2020 18:46:22: 36000000 INFO @ Sun, 21 Jun 2020 18:46:26: 36000000 INFO @ Sun, 21 Jun 2020 18:46:28: 37000000 INFO @ Sun, 21 Jun 2020 18:46:32: 37000000 INFO @ Sun, 21 Jun 2020 18:46:35: 38000000 INFO @ Sun, 21 Jun 2020 18:46:38: 38000000 INFO @ Sun, 21 Jun 2020 18:46:42: 39000000 INFO @ Sun, 21 Jun 2020 18:46:45: 39000000 INFO @ Sun, 21 Jun 2020 18:46:48: 40000000 INFO @ Sun, 21 Jun 2020 18:46:51: 40000000 INFO @ Sun, 21 Jun 2020 18:46:55: 41000000 INFO @ Sun, 21 Jun 2020 18:46:57: 41000000 INFO @ Sun, 21 Jun 2020 18:46:58: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:46:58: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:46:58: #1 total tags in treatment: 41451009 INFO @ Sun, 21 Jun 2020 18:46:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:46:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:46:59: #1 tags after filtering in treatment: 41451009 INFO @ Sun, 21 Jun 2020 18:46:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:46:59: #1 finished! INFO @ Sun, 21 Jun 2020 18:46:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:46:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:47:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:47:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:47:00: #1 total tags in treatment: 41451009 INFO @ Sun, 21 Jun 2020 18:47:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:47:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:47:01: #1 tags after filtering in treatment: 41451009 INFO @ Sun, 21 Jun 2020 18:47:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:47:01: #1 finished! INFO @ Sun, 21 Jun 2020 18:47:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:47:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:47:02: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 18:47:02: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:47:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:47:04: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 18:47:04: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:47:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135521/SRX135521.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。