Job ID = 6453657 SRX = SRX135520 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:34:09 prefetch.2.10.7: 1) Downloading 'SRR453245'... 2020-06-21T08:34:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:46:07 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:46:07 prefetch.2.10.7: 1) 'SRR453245' was downloaded successfully Read 81232080 spots for SRR453245/SRR453245.sra Written 81232080 spots for SRR453245/SRR453245.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:47 81232080 reads; of these: 81232080 (100.00%) were unpaired; of these: 5321884 (6.55%) aligned 0 times 69376482 (85.41%) aligned exactly 1 time 6533714 (8.04%) aligned >1 times 93.45% overall alignment rate Time searching: 00:20:47 Overall time: 00:20:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 38045583 / 75910196 = 0.5012 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:26:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:26:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:26:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:26:06: 1000000 INFO @ Sun, 21 Jun 2020 18:26:12: 2000000 INFO @ Sun, 21 Jun 2020 18:26:18: 3000000 INFO @ Sun, 21 Jun 2020 18:26:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:26:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:26:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:26:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:26:31: 5000000 INFO @ Sun, 21 Jun 2020 18:26:38: 1000000 INFO @ Sun, 21 Jun 2020 18:26:39: 6000000 INFO @ Sun, 21 Jun 2020 18:26:46: 2000000 INFO @ Sun, 21 Jun 2020 18:26:46: 7000000 INFO @ Sun, 21 Jun 2020 18:26:54: 3000000 INFO @ Sun, 21 Jun 2020 18:26:54: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:27:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:27:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:27:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:27:02: 4000000 INFO @ Sun, 21 Jun 2020 18:27:02: 9000000 INFO @ Sun, 21 Jun 2020 18:27:09: 1000000 INFO @ Sun, 21 Jun 2020 18:27:10: 5000000 INFO @ Sun, 21 Jun 2020 18:27:11: 10000000 INFO @ Sun, 21 Jun 2020 18:27:17: 2000000 INFO @ Sun, 21 Jun 2020 18:27:18: 6000000 INFO @ Sun, 21 Jun 2020 18:27:19: 11000000 INFO @ Sun, 21 Jun 2020 18:27:25: 3000000 INFO @ Sun, 21 Jun 2020 18:27:27: 7000000 INFO @ Sun, 21 Jun 2020 18:27:27: 12000000 INFO @ Sun, 21 Jun 2020 18:27:33: 4000000 INFO @ Sun, 21 Jun 2020 18:27:36: 13000000 INFO @ Sun, 21 Jun 2020 18:27:36: 8000000 INFO @ Sun, 21 Jun 2020 18:27:42: 5000000 INFO @ Sun, 21 Jun 2020 18:27:44: 14000000 INFO @ Sun, 21 Jun 2020 18:27:44: 9000000 INFO @ Sun, 21 Jun 2020 18:27:50: 6000000 INFO @ Sun, 21 Jun 2020 18:27:52: 15000000 INFO @ Sun, 21 Jun 2020 18:27:52: 10000000 INFO @ Sun, 21 Jun 2020 18:27:58: 7000000 INFO @ Sun, 21 Jun 2020 18:28:00: 16000000 INFO @ Sun, 21 Jun 2020 18:28:01: 11000000 INFO @ Sun, 21 Jun 2020 18:28:07: 8000000 INFO @ Sun, 21 Jun 2020 18:28:09: 17000000 INFO @ Sun, 21 Jun 2020 18:28:09: 12000000 INFO @ Sun, 21 Jun 2020 18:28:15: 9000000 INFO @ Sun, 21 Jun 2020 18:28:17: 18000000 INFO @ Sun, 21 Jun 2020 18:28:18: 13000000 INFO @ Sun, 21 Jun 2020 18:28:23: 10000000 INFO @ Sun, 21 Jun 2020 18:28:25: 19000000 INFO @ Sun, 21 Jun 2020 18:28:26: 14000000 INFO @ Sun, 21 Jun 2020 18:28:31: 11000000 INFO @ Sun, 21 Jun 2020 18:28:33: 20000000 INFO @ Sun, 21 Jun 2020 18:28:34: 15000000 INFO @ Sun, 21 Jun 2020 18:28:39: 12000000 INFO @ Sun, 21 Jun 2020 18:28:41: 21000000 INFO @ Sun, 21 Jun 2020 18:28:43: 16000000 INFO @ Sun, 21 Jun 2020 18:28:47: 13000000 INFO @ Sun, 21 Jun 2020 18:28:49: 22000000 INFO @ Sun, 21 Jun 2020 18:28:51: 17000000 INFO @ Sun, 21 Jun 2020 18:28:55: 14000000 INFO @ Sun, 21 Jun 2020 18:28:57: 23000000 INFO @ Sun, 21 Jun 2020 18:28:59: 18000000 INFO @ Sun, 21 Jun 2020 18:29:03: 15000000 INFO @ Sun, 21 Jun 2020 18:29:05: 24000000 INFO @ Sun, 21 Jun 2020 18:29:07: 19000000 INFO @ Sun, 21 Jun 2020 18:29:11: 16000000 INFO @ Sun, 21 Jun 2020 18:29:13: 25000000 INFO @ Sun, 21 Jun 2020 18:29:16: 20000000 INFO @ Sun, 21 Jun 2020 18:29:19: 17000000 INFO @ Sun, 21 Jun 2020 18:29:21: 26000000 INFO @ Sun, 21 Jun 2020 18:29:24: 21000000 INFO @ Sun, 21 Jun 2020 18:29:27: 18000000 INFO @ Sun, 21 Jun 2020 18:29:29: 27000000 INFO @ Sun, 21 Jun 2020 18:29:33: 22000000 INFO @ Sun, 21 Jun 2020 18:29:35: 19000000 INFO @ Sun, 21 Jun 2020 18:29:37: 28000000 INFO @ Sun, 21 Jun 2020 18:29:41: 23000000 INFO @ Sun, 21 Jun 2020 18:29:43: 20000000 INFO @ Sun, 21 Jun 2020 18:29:45: 29000000 INFO @ Sun, 21 Jun 2020 18:29:50: 24000000 INFO @ Sun, 21 Jun 2020 18:29:51: 21000000 INFO @ Sun, 21 Jun 2020 18:29:53: 30000000 INFO @ Sun, 21 Jun 2020 18:29:58: 25000000 INFO @ Sun, 21 Jun 2020 18:30:00: 22000000 INFO @ Sun, 21 Jun 2020 18:30:01: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:30:07: 26000000 INFO @ Sun, 21 Jun 2020 18:30:08: 23000000 INFO @ Sun, 21 Jun 2020 18:30:10: 32000000 INFO @ Sun, 21 Jun 2020 18:30:15: 27000000 INFO @ Sun, 21 Jun 2020 18:30:16: 24000000 INFO @ Sun, 21 Jun 2020 18:30:18: 33000000 INFO @ Sun, 21 Jun 2020 18:30:23: 28000000 INFO @ Sun, 21 Jun 2020 18:30:24: 25000000 INFO @ Sun, 21 Jun 2020 18:30:26: 34000000 INFO @ Sun, 21 Jun 2020 18:30:31: 29000000 INFO @ Sun, 21 Jun 2020 18:30:32: 26000000 INFO @ Sun, 21 Jun 2020 18:30:33: 35000000 INFO @ Sun, 21 Jun 2020 18:30:40: 30000000 INFO @ Sun, 21 Jun 2020 18:30:40: 27000000 INFO @ Sun, 21 Jun 2020 18:30:41: 36000000 INFO @ Sun, 21 Jun 2020 18:30:47: 28000000 INFO @ Sun, 21 Jun 2020 18:30:48: 31000000 INFO @ Sun, 21 Jun 2020 18:30:49: 37000000 INFO @ Sun, 21 Jun 2020 18:30:55: 29000000 INFO @ Sun, 21 Jun 2020 18:30:57: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:30:57: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:30:57: #1 total tags in treatment: 37864613 INFO @ Sun, 21 Jun 2020 18:30:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:30:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:30:57: 32000000 INFO @ Sun, 21 Jun 2020 18:30:58: #1 tags after filtering in treatment: 37864606 INFO @ Sun, 21 Jun 2020 18:30:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:30:58: #1 finished! INFO @ Sun, 21 Jun 2020 18:30:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:30:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:31:00: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:31:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:31:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:31:03: 30000000 INFO @ Sun, 21 Jun 2020 18:31:05: 33000000 INFO @ Sun, 21 Jun 2020 18:31:11: 31000000 INFO @ Sun, 21 Jun 2020 18:31:14: 34000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:31:19: 32000000 INFO @ Sun, 21 Jun 2020 18:31:22: 35000000 INFO @ Sun, 21 Jun 2020 18:31:27: 33000000 INFO @ Sun, 21 Jun 2020 18:31:30: 36000000 INFO @ Sun, 21 Jun 2020 18:31:35: 34000000 INFO @ Sun, 21 Jun 2020 18:31:39: 37000000 INFO @ Sun, 21 Jun 2020 18:31:43: 35000000 INFO @ Sun, 21 Jun 2020 18:31:46: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:31:46: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:31:46: #1 total tags in treatment: 37864613 INFO @ Sun, 21 Jun 2020 18:31:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:31:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:31:47: #1 tags after filtering in treatment: 37864606 INFO @ Sun, 21 Jun 2020 18:31:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:31:47: #1 finished! INFO @ Sun, 21 Jun 2020 18:31:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:31:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:31:50: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:31:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:31:50: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 18:31:50: 36000000 cut: /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:31:57: 37000000 INFO @ Sun, 21 Jun 2020 18:32:03: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:32:03: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:32:03: #1 total tags in treatment: 37864613 INFO @ Sun, 21 Jun 2020 18:32:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:32:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:32:04: #1 tags after filtering in treatment: 37864606 INFO @ Sun, 21 Jun 2020 18:32:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:32:04: #1 finished! INFO @ Sun, 21 Jun 2020 18:32:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:32:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:32:06: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:32:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:32:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135520/SRX135520.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling