Job ID = 6453656 SRX = SRX135519 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:37:09 prefetch.2.10.7: 1) Downloading 'SRR453244'... 2020-06-21T08:37:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:12:25 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:12:25 prefetch.2.10.7: 1) 'SRR453244' was downloaded successfully Read 152726695 spots for SRR453244/SRR453244.sra Written 152726695 spots for SRR453244/SRR453244.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:34:57 152726695 reads; of these: 152726695 (100.00%) were unpaired; of these: 22020296 (14.42%) aligned 0 times 119312501 (78.12%) aligned exactly 1 time 11393898 (7.46%) aligned >1 times 85.58% overall alignment rate Time searching: 00:34:57 Overall time: 00:34:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 56 files... [bam_rmdupse_core] 78189923 / 130706399 = 0.5982 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:22:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:22:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:22:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:22:55: 1000000 INFO @ Sun, 21 Jun 2020 19:23:02: 2000000 INFO @ Sun, 21 Jun 2020 19:23:09: 3000000 INFO @ Sun, 21 Jun 2020 19:23:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:23:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:23:19: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:23:19: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:23:23: 5000000 INFO @ Sun, 21 Jun 2020 19:23:26: 1000000 INFO @ Sun, 21 Jun 2020 19:23:30: 6000000 INFO @ Sun, 21 Jun 2020 19:23:34: 2000000 INFO @ Sun, 21 Jun 2020 19:23:37: 7000000 INFO @ Sun, 21 Jun 2020 19:23:40: 3000000 INFO @ Sun, 21 Jun 2020 19:23:43: 8000000 INFO @ Sun, 21 Jun 2020 19:23:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:23:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:23:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:23:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:23:49: 9000000 INFO @ Sun, 21 Jun 2020 19:23:53: 5000000 INFO @ Sun, 21 Jun 2020 19:23:55: 1000000 INFO @ Sun, 21 Jun 2020 19:23:55: 10000000 INFO @ Sun, 21 Jun 2020 19:23:59: 6000000 INFO @ Sun, 21 Jun 2020 19:24:01: 2000000 INFO @ Sun, 21 Jun 2020 19:24:01: 11000000 INFO @ Sun, 21 Jun 2020 19:24:06: 7000000 INFO @ Sun, 21 Jun 2020 19:24:07: 3000000 INFO @ Sun, 21 Jun 2020 19:24:07: 12000000 INFO @ Sun, 21 Jun 2020 19:24:12: 8000000 INFO @ Sun, 21 Jun 2020 19:24:13: 4000000 INFO @ Sun, 21 Jun 2020 19:24:13: 13000000 INFO @ Sun, 21 Jun 2020 19:24:18: 9000000 INFO @ Sun, 21 Jun 2020 19:24:19: 5000000 INFO @ Sun, 21 Jun 2020 19:24:20: 14000000 INFO @ Sun, 21 Jun 2020 19:24:25: 10000000 INFO @ Sun, 21 Jun 2020 19:24:25: 6000000 INFO @ Sun, 21 Jun 2020 19:24:27: 15000000 INFO @ Sun, 21 Jun 2020 19:24:31: 7000000 INFO @ Sun, 21 Jun 2020 19:24:32: 11000000 INFO @ Sun, 21 Jun 2020 19:24:34: 16000000 INFO @ Sun, 21 Jun 2020 19:24:38: 8000000 INFO @ Sun, 21 Jun 2020 19:24:40: 12000000 INFO @ Sun, 21 Jun 2020 19:24:41: 17000000 INFO @ Sun, 21 Jun 2020 19:24:44: 9000000 INFO @ Sun, 21 Jun 2020 19:24:48: 13000000 INFO @ Sun, 21 Jun 2020 19:24:49: 18000000 INFO @ Sun, 21 Jun 2020 19:24:50: 10000000 INFO @ Sun, 21 Jun 2020 19:24:55: 14000000 INFO @ Sun, 21 Jun 2020 19:24:56: 19000000 INFO @ Sun, 21 Jun 2020 19:24:56: 11000000 INFO @ Sun, 21 Jun 2020 19:25:02: 15000000 INFO @ Sun, 21 Jun 2020 19:25:02: 20000000 INFO @ Sun, 21 Jun 2020 19:25:02: 12000000 INFO @ Sun, 21 Jun 2020 19:25:09: 13000000 INFO @ Sun, 21 Jun 2020 19:25:09: 16000000 INFO @ Sun, 21 Jun 2020 19:25:09: 21000000 INFO @ Sun, 21 Jun 2020 19:25:15: 14000000 INFO @ Sun, 21 Jun 2020 19:25:16: 17000000 INFO @ Sun, 21 Jun 2020 19:25:17: 22000000 INFO @ Sun, 21 Jun 2020 19:25:21: 15000000 INFO @ Sun, 21 Jun 2020 19:25:23: 18000000 INFO @ Sun, 21 Jun 2020 19:25:24: 23000000 INFO @ Sun, 21 Jun 2020 19:25:27: 16000000 INFO @ Sun, 21 Jun 2020 19:25:30: 19000000 INFO @ Sun, 21 Jun 2020 19:25:31: 24000000 INFO @ Sun, 21 Jun 2020 19:25:33: 17000000 INFO @ Sun, 21 Jun 2020 19:25:37: 20000000 INFO @ Sun, 21 Jun 2020 19:25:38: 25000000 INFO @ Sun, 21 Jun 2020 19:25:39: 18000000 INFO @ Sun, 21 Jun 2020 19:25:45: 26000000 INFO @ Sun, 21 Jun 2020 19:25:45: 21000000 INFO @ Sun, 21 Jun 2020 19:25:45: 19000000 INFO @ Sun, 21 Jun 2020 19:25:51: 20000000 INFO @ Sun, 21 Jun 2020 19:25:51: 27000000 INFO @ Sun, 21 Jun 2020 19:25:52: 22000000 INFO @ Sun, 21 Jun 2020 19:25:57: 21000000 INFO @ Sun, 21 Jun 2020 19:25:58: 28000000 INFO @ Sun, 21 Jun 2020 19:25:59: 23000000 INFO @ Sun, 21 Jun 2020 19:26:03: 22000000 INFO @ Sun, 21 Jun 2020 19:26:05: 29000000 INFO @ Sun, 21 Jun 2020 19:26:05: 24000000 INFO @ Sun, 21 Jun 2020 19:26:09: 23000000 INFO @ Sun, 21 Jun 2020 19:26:11: 30000000 INFO @ Sun, 21 Jun 2020 19:26:12: 25000000 INFO @ Sun, 21 Jun 2020 19:26:15: 24000000 INFO @ Sun, 21 Jun 2020 19:26:18: 31000000 INFO @ Sun, 21 Jun 2020 19:26:19: 26000000 INFO @ Sun, 21 Jun 2020 19:26:21: 25000000 INFO @ Sun, 21 Jun 2020 19:26:25: 32000000 INFO @ Sun, 21 Jun 2020 19:26:26: 27000000 INFO @ Sun, 21 Jun 2020 19:26:27: 26000000 INFO @ Sun, 21 Jun 2020 19:26:31: 33000000 INFO @ Sun, 21 Jun 2020 19:26:33: 28000000 INFO @ Sun, 21 Jun 2020 19:26:33: 27000000 INFO @ Sun, 21 Jun 2020 19:26:37: 34000000 INFO @ Sun, 21 Jun 2020 19:26:39: 28000000 INFO @ Sun, 21 Jun 2020 19:26:40: 29000000 INFO @ Sun, 21 Jun 2020 19:26:43: 35000000 INFO @ Sun, 21 Jun 2020 19:26:46: 29000000 INFO @ Sun, 21 Jun 2020 19:26:46: 30000000 INFO @ Sun, 21 Jun 2020 19:26:50: 36000000 INFO @ Sun, 21 Jun 2020 19:26:52: 30000000 INFO @ Sun, 21 Jun 2020 19:26:54: 31000000 INFO @ Sun, 21 Jun 2020 19:26:57: 37000000 INFO @ Sun, 21 Jun 2020 19:26:58: 31000000 INFO @ Sun, 21 Jun 2020 19:27:01: 32000000 INFO @ Sun, 21 Jun 2020 19:27:03: 38000000 INFO @ Sun, 21 Jun 2020 19:27:04: 32000000 INFO @ Sun, 21 Jun 2020 19:27:08: 33000000 INFO @ Sun, 21 Jun 2020 19:27:10: 33000000 INFO @ Sun, 21 Jun 2020 19:27:10: 39000000 INFO @ Sun, 21 Jun 2020 19:27:14: 34000000 INFO @ Sun, 21 Jun 2020 19:27:16: 34000000 INFO @ Sun, 21 Jun 2020 19:27:17: 40000000 INFO @ Sun, 21 Jun 2020 19:27:21: 35000000 INFO @ Sun, 21 Jun 2020 19:27:22: 35000000 INFO @ Sun, 21 Jun 2020 19:27:24: 41000000 INFO @ Sun, 21 Jun 2020 19:27:28: 36000000 INFO @ Sun, 21 Jun 2020 19:27:28: 36000000 INFO @ Sun, 21 Jun 2020 19:27:31: 42000000 INFO @ Sun, 21 Jun 2020 19:27:35: 37000000 INFO @ Sun, 21 Jun 2020 19:27:36: 37000000 INFO @ Sun, 21 Jun 2020 19:27:37: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:27:42: 38000000 INFO @ Sun, 21 Jun 2020 19:27:43: 38000000 INFO @ Sun, 21 Jun 2020 19:27:44: 44000000 INFO @ Sun, 21 Jun 2020 19:27:49: 39000000 INFO @ Sun, 21 Jun 2020 19:27:50: 39000000 INFO @ Sun, 21 Jun 2020 19:27:51: 45000000 INFO @ Sun, 21 Jun 2020 19:27:57: 40000000 INFO @ Sun, 21 Jun 2020 19:27:57: 40000000 INFO @ Sun, 21 Jun 2020 19:27:58: 46000000 INFO @ Sun, 21 Jun 2020 19:28:03: 41000000 INFO @ Sun, 21 Jun 2020 19:28:04: 41000000 INFO @ Sun, 21 Jun 2020 19:28:05: 47000000 INFO @ Sun, 21 Jun 2020 19:28:10: 42000000 INFO @ Sun, 21 Jun 2020 19:28:11: 42000000 INFO @ Sun, 21 Jun 2020 19:28:12: 48000000 INFO @ Sun, 21 Jun 2020 19:28:17: 43000000 INFO @ Sun, 21 Jun 2020 19:28:17: 43000000 INFO @ Sun, 21 Jun 2020 19:28:19: 49000000 INFO @ Sun, 21 Jun 2020 19:28:24: 44000000 INFO @ Sun, 21 Jun 2020 19:28:24: 44000000 INFO @ Sun, 21 Jun 2020 19:28:26: 50000000 INFO @ Sun, 21 Jun 2020 19:28:30: 45000000 INFO @ Sun, 21 Jun 2020 19:28:31: 45000000 INFO @ Sun, 21 Jun 2020 19:28:33: 51000000 INFO @ Sun, 21 Jun 2020 19:28:37: 46000000 INFO @ Sun, 21 Jun 2020 19:28:37: 46000000 INFO @ Sun, 21 Jun 2020 19:28:40: 52000000 INFO @ Sun, 21 Jun 2020 19:28:43: 47000000 INFO @ Sun, 21 Jun 2020 19:28:44: 47000000 INFO @ Sun, 21 Jun 2020 19:28:44: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:28:44: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:28:44: #1 total tags in treatment: 52516476 INFO @ Sun, 21 Jun 2020 19:28:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:28:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:28:46: #1 tags after filtering in treatment: 52516474 INFO @ Sun, 21 Jun 2020 19:28:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:28:46: #1 finished! INFO @ Sun, 21 Jun 2020 19:28:46: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:28:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:28:49: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:28:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:28:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:28:50: 48000000 INFO @ Sun, 21 Jun 2020 19:28:50: 48000000 INFO @ Sun, 21 Jun 2020 19:28:57: 49000000 INFO @ Sun, 21 Jun 2020 19:28:57: 49000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:29:04: 50000000 INFO @ Sun, 21 Jun 2020 19:29:04: 50000000 INFO @ Sun, 21 Jun 2020 19:29:11: 51000000 INFO @ Sun, 21 Jun 2020 19:29:11: 51000000 INFO @ Sun, 21 Jun 2020 19:29:18: 52000000 INFO @ Sun, 21 Jun 2020 19:29:18: 52000000 INFO @ Sun, 21 Jun 2020 19:29:22: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:29:22: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:29:22: #1 total tags in treatment: 52516476 INFO @ Sun, 21 Jun 2020 19:29:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:29:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:29:22: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:29:22: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:29:22: #1 total tags in treatment: 52516476 INFO @ Sun, 21 Jun 2020 19:29:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:29:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:29:23: #1 tags after filtering in treatment: 52516474 INFO @ Sun, 21 Jun 2020 19:29:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:29:23: #1 finished! INFO @ Sun, 21 Jun 2020 19:29:23: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:29:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:29:23: #1 tags after filtering in treatment: 52516474 INFO @ Sun, 21 Jun 2020 19:29:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:29:23: #1 finished! INFO @ Sun, 21 Jun 2020 19:29:23: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:29:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:29:26: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:29:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:29:26: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 19:29:26: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:29:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:29:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling cut: /home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135519/SRX135519.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling