Job ID = 6453654 SRX = SRX135517 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:34:54 prefetch.2.10.7: 1) Downloading 'SRR453242'... 2020-06-21T08:34:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:53:52 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:53:52 prefetch.2.10.7: 1) 'SRR453242' was downloaded successfully Read 49215113 spots for SRR453242/SRR453242.sra Written 49215113 spots for SRR453242/SRR453242.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:22 49215113 reads; of these: 49215113 (100.00%) were unpaired; of these: 5567619 (11.31%) aligned 0 times 36688207 (74.55%) aligned exactly 1 time 6959287 (14.14%) aligned >1 times 88.69% overall alignment rate Time searching: 00:12:22 Overall time: 00:12:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 18905179 / 43647494 = 0.4331 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:44:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:44:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:44:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:45:03: 1000000 INFO @ Sun, 21 Jun 2020 18:45:08: 2000000 INFO @ Sun, 21 Jun 2020 18:45:13: 3000000 INFO @ Sun, 21 Jun 2020 18:45:18: 4000000 INFO @ Sun, 21 Jun 2020 18:45:24: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:45:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:45:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:45:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:45:29: 6000000 INFO @ Sun, 21 Jun 2020 18:45:34: 7000000 INFO @ Sun, 21 Jun 2020 18:45:34: 1000000 INFO @ Sun, 21 Jun 2020 18:45:40: 8000000 INFO @ Sun, 21 Jun 2020 18:45:40: 2000000 INFO @ Sun, 21 Jun 2020 18:45:45: 9000000 INFO @ Sun, 21 Jun 2020 18:45:46: 3000000 INFO @ Sun, 21 Jun 2020 18:45:50: 10000000 INFO @ Sun, 21 Jun 2020 18:45:51: 4000000 INFO @ Sun, 21 Jun 2020 18:45:56: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:45:57: 5000000 INFO @ Sun, 21 Jun 2020 18:46:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:46:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:46:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:46:01: 12000000 INFO @ Sun, 21 Jun 2020 18:46:03: 6000000 INFO @ Sun, 21 Jun 2020 18:46:06: 1000000 INFO @ Sun, 21 Jun 2020 18:46:07: 13000000 INFO @ Sun, 21 Jun 2020 18:46:09: 7000000 INFO @ Sun, 21 Jun 2020 18:46:12: 2000000 INFO @ Sun, 21 Jun 2020 18:46:12: 14000000 INFO @ Sun, 21 Jun 2020 18:46:15: 8000000 INFO @ Sun, 21 Jun 2020 18:46:17: 3000000 INFO @ Sun, 21 Jun 2020 18:46:18: 15000000 INFO @ Sun, 21 Jun 2020 18:46:20: 9000000 INFO @ Sun, 21 Jun 2020 18:46:22: 4000000 INFO @ Sun, 21 Jun 2020 18:46:23: 16000000 INFO @ Sun, 21 Jun 2020 18:46:26: 10000000 INFO @ Sun, 21 Jun 2020 18:46:28: 5000000 INFO @ Sun, 21 Jun 2020 18:46:28: 17000000 INFO @ Sun, 21 Jun 2020 18:46:32: 11000000 INFO @ Sun, 21 Jun 2020 18:46:33: 6000000 INFO @ Sun, 21 Jun 2020 18:46:34: 18000000 INFO @ Sun, 21 Jun 2020 18:46:38: 12000000 INFO @ Sun, 21 Jun 2020 18:46:39: 7000000 INFO @ Sun, 21 Jun 2020 18:46:39: 19000000 INFO @ Sun, 21 Jun 2020 18:46:44: 13000000 INFO @ Sun, 21 Jun 2020 18:46:44: 8000000 INFO @ Sun, 21 Jun 2020 18:46:44: 20000000 INFO @ Sun, 21 Jun 2020 18:46:49: 9000000 INFO @ Sun, 21 Jun 2020 18:46:50: 14000000 INFO @ Sun, 21 Jun 2020 18:46:50: 21000000 INFO @ Sun, 21 Jun 2020 18:46:55: 10000000 INFO @ Sun, 21 Jun 2020 18:46:55: 22000000 INFO @ Sun, 21 Jun 2020 18:46:56: 15000000 INFO @ Sun, 21 Jun 2020 18:47:00: 11000000 INFO @ Sun, 21 Jun 2020 18:47:01: 23000000 INFO @ Sun, 21 Jun 2020 18:47:01: 16000000 INFO @ Sun, 21 Jun 2020 18:47:06: 12000000 INFO @ Sun, 21 Jun 2020 18:47:06: 24000000 INFO @ Sun, 21 Jun 2020 18:47:07: 17000000 INFO @ Sun, 21 Jun 2020 18:47:10: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:47:10: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:47:10: #1 total tags in treatment: 24742315 INFO @ Sun, 21 Jun 2020 18:47:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:47:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:47:11: #1 tags after filtering in treatment: 24742315 INFO @ Sun, 21 Jun 2020 18:47:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:47:11: #1 finished! INFO @ Sun, 21 Jun 2020 18:47:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:47:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:47:11: 13000000 INFO @ Sun, 21 Jun 2020 18:47:13: #2 number of paired peaks: 11 WARNING @ Sun, 21 Jun 2020 18:47:13: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:47:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:47:13: 18000000 INFO @ Sun, 21 Jun 2020 18:47:16: 14000000 INFO @ Sun, 21 Jun 2020 18:47:19: 19000000 INFO @ Sun, 21 Jun 2020 18:47:22: 15000000 INFO @ Sun, 21 Jun 2020 18:47:24: 20000000 INFO @ Sun, 21 Jun 2020 18:47:27: 16000000 INFO @ Sun, 21 Jun 2020 18:47:31: 21000000 INFO @ Sun, 21 Jun 2020 18:47:32: 17000000 INFO @ Sun, 21 Jun 2020 18:47:37: 22000000 INFO @ Sun, 21 Jun 2020 18:47:38: 18000000 INFO @ Sun, 21 Jun 2020 18:47:42: 23000000 INFO @ Sun, 21 Jun 2020 18:47:43: 19000000 INFO @ Sun, 21 Jun 2020 18:47:48: 24000000 INFO @ Sun, 21 Jun 2020 18:47:49: 20000000 INFO @ Sun, 21 Jun 2020 18:47:53: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:47:53: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:47:53: #1 total tags in treatment: 24742315 INFO @ Sun, 21 Jun 2020 18:47:53: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:47:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:47:54: #1 tags after filtering in treatment: 24742315 INFO @ Sun, 21 Jun 2020 18:47:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:47:54: #1 finished! INFO @ Sun, 21 Jun 2020 18:47:54: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:47:54: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:47:54: 21000000 INFO @ Sun, 21 Jun 2020 18:47:55: #2 number of paired peaks: 11 WARNING @ Sun, 21 Jun 2020 18:47:55: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:47:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:48:00: 22000000 INFO @ Sun, 21 Jun 2020 18:48:05: 23000000 INFO @ Sun, 21 Jun 2020 18:48:09: 24000000 INFO @ Sun, 21 Jun 2020 18:48:14: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:48:14: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:48:14: #1 total tags in treatment: 24742315 INFO @ Sun, 21 Jun 2020 18:48:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:48:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:48:14: #1 tags after filtering in treatment: 24742315 INFO @ Sun, 21 Jun 2020 18:48:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:48:14: #1 finished! INFO @ Sun, 21 Jun 2020 18:48:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:48:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:48:16: #2 number of paired peaks: 11 WARNING @ Sun, 21 Jun 2020 18:48:16: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:48:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX135517/SRX135517.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。