Job ID = 6453651 SRX = SRX1342248 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:50:19 prefetch.2.10.7: 1) Downloading 'SRR2672987'... 2020-06-21T08:50:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:03:40 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:03:40 prefetch.2.10.7: 1) 'SRR2672987' was downloaded successfully Read 79019649 spots for SRR2672987/SRR2672987.sra Written 79019649 spots for SRR2672987/SRR2672987.sra 2020-06-21T09:08:04 prefetch.2.10.7: 1) Downloading 'SRR2672988'... 2020-06-21T09:08:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:20:36 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:20:36 prefetch.2.10.7: 1) 'SRR2672988' was downloaded successfully Read 91027136 spots for SRR2672988/SRR2672988.sra Written 91027136 spots for SRR2672988/SRR2672988.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:39 170046785 reads; of these: 170046785 (100.00%) were unpaired; of these: 11448717 (6.73%) aligned 0 times 130424761 (76.70%) aligned exactly 1 time 28173307 (16.57%) aligned >1 times 93.27% overall alignment rate Time searching: 00:32:39 Overall time: 00:32:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 104691482 / 158598068 = 0.6601 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:27:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:27:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:27:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:27:22: 1000000 INFO @ Sun, 21 Jun 2020 19:27:26: 2000000 INFO @ Sun, 21 Jun 2020 19:27:31: 3000000 INFO @ Sun, 21 Jun 2020 19:27:36: 4000000 INFO @ Sun, 21 Jun 2020 19:27:40: 5000000 INFO @ Sun, 21 Jun 2020 19:27:45: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:27:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:27:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:27:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:27:49: 7000000 INFO @ Sun, 21 Jun 2020 19:27:52: 1000000 INFO @ Sun, 21 Jun 2020 19:27:54: 8000000 INFO @ Sun, 21 Jun 2020 19:27:57: 2000000 INFO @ Sun, 21 Jun 2020 19:27:58: 9000000 INFO @ Sun, 21 Jun 2020 19:28:01: 3000000 INFO @ Sun, 21 Jun 2020 19:28:03: 10000000 INFO @ Sun, 21 Jun 2020 19:28:06: 4000000 INFO @ Sun, 21 Jun 2020 19:28:08: 11000000 INFO @ Sun, 21 Jun 2020 19:28:11: 5000000 INFO @ Sun, 21 Jun 2020 19:28:12: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:28:15: 6000000 INFO @ Sun, 21 Jun 2020 19:28:17: 13000000 INFO @ Sun, 21 Jun 2020 19:28:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:28:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:28:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:28:20: 7000000 INFO @ Sun, 21 Jun 2020 19:28:22: 14000000 INFO @ Sun, 21 Jun 2020 19:28:22: 1000000 INFO @ Sun, 21 Jun 2020 19:28:26: 8000000 INFO @ Sun, 21 Jun 2020 19:28:28: 15000000 INFO @ Sun, 21 Jun 2020 19:28:28: 2000000 INFO @ Sun, 21 Jun 2020 19:28:31: 9000000 INFO @ Sun, 21 Jun 2020 19:28:33: 16000000 INFO @ Sun, 21 Jun 2020 19:28:33: 3000000 INFO @ Sun, 21 Jun 2020 19:28:36: 10000000 INFO @ Sun, 21 Jun 2020 19:28:38: 17000000 INFO @ Sun, 21 Jun 2020 19:28:38: 4000000 INFO @ Sun, 21 Jun 2020 19:28:41: 11000000 INFO @ Sun, 21 Jun 2020 19:28:43: 18000000 INFO @ Sun, 21 Jun 2020 19:28:43: 5000000 INFO @ Sun, 21 Jun 2020 19:28:47: 12000000 INFO @ Sun, 21 Jun 2020 19:28:49: 19000000 INFO @ Sun, 21 Jun 2020 19:28:49: 6000000 INFO @ Sun, 21 Jun 2020 19:28:52: 13000000 INFO @ Sun, 21 Jun 2020 19:28:54: 20000000 INFO @ Sun, 21 Jun 2020 19:28:54: 7000000 INFO @ Sun, 21 Jun 2020 19:28:57: 14000000 INFO @ Sun, 21 Jun 2020 19:28:59: 21000000 INFO @ Sun, 21 Jun 2020 19:28:59: 8000000 INFO @ Sun, 21 Jun 2020 19:29:03: 15000000 INFO @ Sun, 21 Jun 2020 19:29:04: 22000000 INFO @ Sun, 21 Jun 2020 19:29:04: 9000000 INFO @ Sun, 21 Jun 2020 19:29:08: 16000000 INFO @ Sun, 21 Jun 2020 19:29:09: 23000000 INFO @ Sun, 21 Jun 2020 19:29:09: 10000000 INFO @ Sun, 21 Jun 2020 19:29:13: 17000000 INFO @ Sun, 21 Jun 2020 19:29:14: 24000000 INFO @ Sun, 21 Jun 2020 19:29:15: 11000000 INFO @ Sun, 21 Jun 2020 19:29:19: 18000000 INFO @ Sun, 21 Jun 2020 19:29:20: 25000000 INFO @ Sun, 21 Jun 2020 19:29:20: 12000000 INFO @ Sun, 21 Jun 2020 19:29:24: 19000000 INFO @ Sun, 21 Jun 2020 19:29:25: 26000000 INFO @ Sun, 21 Jun 2020 19:29:25: 13000000 INFO @ Sun, 21 Jun 2020 19:29:29: 20000000 INFO @ Sun, 21 Jun 2020 19:29:30: 27000000 INFO @ Sun, 21 Jun 2020 19:29:30: 14000000 INFO @ Sun, 21 Jun 2020 19:29:34: 21000000 INFO @ Sun, 21 Jun 2020 19:29:35: 28000000 INFO @ Sun, 21 Jun 2020 19:29:35: 15000000 INFO @ Sun, 21 Jun 2020 19:29:40: 22000000 INFO @ Sun, 21 Jun 2020 19:29:40: 29000000 INFO @ Sun, 21 Jun 2020 19:29:41: 16000000 INFO @ Sun, 21 Jun 2020 19:29:45: 23000000 INFO @ Sun, 21 Jun 2020 19:29:46: 30000000 INFO @ Sun, 21 Jun 2020 19:29:46: 17000000 INFO @ Sun, 21 Jun 2020 19:29:50: 24000000 INFO @ Sun, 21 Jun 2020 19:29:51: 31000000 INFO @ Sun, 21 Jun 2020 19:29:51: 18000000 INFO @ Sun, 21 Jun 2020 19:29:55: 25000000 INFO @ Sun, 21 Jun 2020 19:29:56: 32000000 INFO @ Sun, 21 Jun 2020 19:29:56: 19000000 INFO @ Sun, 21 Jun 2020 19:30:01: 26000000 INFO @ Sun, 21 Jun 2020 19:30:01: 33000000 INFO @ Sun, 21 Jun 2020 19:30:01: 20000000 INFO @ Sun, 21 Jun 2020 19:30:06: 27000000 INFO @ Sun, 21 Jun 2020 19:30:06: 34000000 INFO @ Sun, 21 Jun 2020 19:30:06: 21000000 INFO @ Sun, 21 Jun 2020 19:30:11: 28000000 INFO @ Sun, 21 Jun 2020 19:30:11: 35000000 INFO @ Sun, 21 Jun 2020 19:30:12: 22000000 INFO @ Sun, 21 Jun 2020 19:30:16: 29000000 INFO @ Sun, 21 Jun 2020 19:30:17: 36000000 INFO @ Sun, 21 Jun 2020 19:30:17: 23000000 INFO @ Sun, 21 Jun 2020 19:30:22: 30000000 INFO @ Sun, 21 Jun 2020 19:30:22: 24000000 INFO @ Sun, 21 Jun 2020 19:30:22: 37000000 INFO @ Sun, 21 Jun 2020 19:30:27: 31000000 INFO @ Sun, 21 Jun 2020 19:30:27: 38000000 INFO @ Sun, 21 Jun 2020 19:30:27: 25000000 INFO @ Sun, 21 Jun 2020 19:30:32: 32000000 INFO @ Sun, 21 Jun 2020 19:30:32: 39000000 INFO @ Sun, 21 Jun 2020 19:30:33: 26000000 INFO @ Sun, 21 Jun 2020 19:30:38: 40000000 INFO @ Sun, 21 Jun 2020 19:30:38: 33000000 INFO @ Sun, 21 Jun 2020 19:30:38: 27000000 INFO @ Sun, 21 Jun 2020 19:30:43: 41000000 INFO @ Sun, 21 Jun 2020 19:30:43: 34000000 INFO @ Sun, 21 Jun 2020 19:30:44: 28000000 INFO @ Sun, 21 Jun 2020 19:30:49: 42000000 INFO @ Sun, 21 Jun 2020 19:30:49: 35000000 INFO @ Sun, 21 Jun 2020 19:30:49: 29000000 INFO @ Sun, 21 Jun 2020 19:30:55: 30000000 INFO @ Sun, 21 Jun 2020 19:30:55: 36000000 INFO @ Sun, 21 Jun 2020 19:30:55: 43000000 INFO @ Sun, 21 Jun 2020 19:31:00: 31000000 INFO @ Sun, 21 Jun 2020 19:31:00: 37000000 INFO @ Sun, 21 Jun 2020 19:31:01: 44000000 INFO @ Sun, 21 Jun 2020 19:31:06: 32000000 INFO @ Sun, 21 Jun 2020 19:31:06: 38000000 INFO @ Sun, 21 Jun 2020 19:31:06: 45000000 INFO @ Sun, 21 Jun 2020 19:31:11: 33000000 INFO @ Sun, 21 Jun 2020 19:31:12: 39000000 INFO @ Sun, 21 Jun 2020 19:31:12: 46000000 INFO @ Sun, 21 Jun 2020 19:31:17: 34000000 INFO @ Sun, 21 Jun 2020 19:31:18: 40000000 INFO @ Sun, 21 Jun 2020 19:31:18: 47000000 INFO @ Sun, 21 Jun 2020 19:31:23: 35000000 INFO @ Sun, 21 Jun 2020 19:31:23: 41000000 INFO @ Sun, 21 Jun 2020 19:31:23: 48000000 INFO @ Sun, 21 Jun 2020 19:31:29: 36000000 INFO @ Sun, 21 Jun 2020 19:31:29: 42000000 INFO @ Sun, 21 Jun 2020 19:31:29: 49000000 INFO @ Sun, 21 Jun 2020 19:31:35: 37000000 INFO @ Sun, 21 Jun 2020 19:31:35: 50000000 INFO @ Sun, 21 Jun 2020 19:31:35: 43000000 INFO @ Sun, 21 Jun 2020 19:31:40: 38000000 INFO @ Sun, 21 Jun 2020 19:31:41: 51000000 INFO @ Sun, 21 Jun 2020 19:31:42: 44000000 INFO @ Sun, 21 Jun 2020 19:31:46: 39000000 INFO @ Sun, 21 Jun 2020 19:31:47: 52000000 INFO @ Sun, 21 Jun 2020 19:31:47: 45000000 INFO @ Sun, 21 Jun 2020 19:31:52: 40000000 INFO @ Sun, 21 Jun 2020 19:31:52: 53000000 INFO @ Sun, 21 Jun 2020 19:31:53: 46000000 INFO @ Sun, 21 Jun 2020 19:31:58: 41000000 INFO @ Sun, 21 Jun 2020 19:31:59: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 19:31:59: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 19:31:59: #1 total tags in treatment: 53906586 INFO @ Sun, 21 Jun 2020 19:31:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:31:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:31:59: 47000000 INFO @ Sun, 21 Jun 2020 19:32:00: #1 tags after filtering in treatment: 53906586 INFO @ Sun, 21 Jun 2020 19:32:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:32:00: #1 finished! INFO @ Sun, 21 Jun 2020 19:32:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:32:00: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:32:03: 42000000 INFO @ Sun, 21 Jun 2020 19:32:03: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:32:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:32:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:32:05: 48000000 INFO @ Sun, 21 Jun 2020 19:32:09: 43000000 INFO @ Sun, 21 Jun 2020 19:32:10: 49000000 INFO @ Sun, 21 Jun 2020 19:32:16: 44000000 INFO @ Sun, 21 Jun 2020 19:32:16: 50000000 INFO @ Sun, 21 Jun 2020 19:32:21: 45000000 INFO @ Sun, 21 Jun 2020 19:32:22: 51000000 INFO @ Sun, 21 Jun 2020 19:32:27: 46000000 INFO @ Sun, 21 Jun 2020 19:32:28: 52000000 INFO @ Sun, 21 Jun 2020 19:32:32: 47000000 INFO @ Sun, 21 Jun 2020 19:32:33: 53000000 INFO @ Sun, 21 Jun 2020 19:32:38: 48000000 INFO @ Sun, 21 Jun 2020 19:32:39: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 19:32:39: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 19:32:39: #1 total tags in treatment: 53906586 INFO @ Sun, 21 Jun 2020 19:32:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:32:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:32:41: #1 tags after filtering in treatment: 53906586 INFO @ Sun, 21 Jun 2020 19:32:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:32:41: #1 finished! INFO @ Sun, 21 Jun 2020 19:32:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:32:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:32:43: 49000000 INFO @ Sun, 21 Jun 2020 19:32:44: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:32:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:32:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:32:48: 50000000 INFO @ Sun, 21 Jun 2020 19:32:53: 51000000 INFO @ Sun, 21 Jun 2020 19:32:58: 52000000 INFO @ Sun, 21 Jun 2020 19:33:03: 53000000 INFO @ Sun, 21 Jun 2020 19:33:09: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 19:33:09: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 19:33:09: #1 total tags in treatment: 53906586 INFO @ Sun, 21 Jun 2020 19:33:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:33:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:33:10: #1 tags after filtering in treatment: 53906586 INFO @ Sun, 21 Jun 2020 19:33:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:33:10: #1 finished! INFO @ Sun, 21 Jun 2020 19:33:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:33:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:33:13: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:33:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:33:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1342248/SRX1342248.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。