Job ID = 6453576 SRX = SRX127436 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:32:26 prefetch.2.10.7: 1) Downloading 'SRR438683'... 2020-06-21T08:32:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:39:38 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:39:38 prefetch.2.10.7: 1) 'SRR438683' was downloaded successfully Read 38326589 spots for SRR438683/SRR438683.sra Written 38326589 spots for SRR438683/SRR438683.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:41 38326589 reads; of these: 38326589 (100.00%) were unpaired; of these: 5506377 (14.37%) aligned 0 times 24492792 (63.91%) aligned exactly 1 time 8327420 (21.73%) aligned >1 times 85.63% overall alignment rate Time searching: 00:07:41 Overall time: 00:07:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5333817 / 32820212 = 0.1625 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:56:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:56:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:56:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:56:16: 1000000 INFO @ Sun, 21 Jun 2020 17:56:21: 2000000 INFO @ Sun, 21 Jun 2020 17:56:26: 3000000 INFO @ Sun, 21 Jun 2020 17:56:32: 4000000 INFO @ Sun, 21 Jun 2020 17:56:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:56:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:56:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:56:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:56:42: 6000000 INFO @ Sun, 21 Jun 2020 17:56:46: 1000000 INFO @ Sun, 21 Jun 2020 17:56:48: 7000000 INFO @ Sun, 21 Jun 2020 17:56:52: 2000000 INFO @ Sun, 21 Jun 2020 17:56:53: 8000000 INFO @ Sun, 21 Jun 2020 17:56:57: 3000000 INFO @ Sun, 21 Jun 2020 17:56:59: 9000000 INFO @ Sun, 21 Jun 2020 17:57:03: 4000000 INFO @ Sun, 21 Jun 2020 17:57:04: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:57:09: 5000000 INFO @ Sun, 21 Jun 2020 17:57:10: 11000000 INFO @ Sun, 21 Jun 2020 17:57:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:57:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:57:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:57:15: 6000000 INFO @ Sun, 21 Jun 2020 17:57:16: 12000000 INFO @ Sun, 21 Jun 2020 17:57:17: 1000000 INFO @ Sun, 21 Jun 2020 17:57:21: 7000000 INFO @ Sun, 21 Jun 2020 17:57:22: 13000000 INFO @ Sun, 21 Jun 2020 17:57:23: 2000000 INFO @ Sun, 21 Jun 2020 17:57:27: 8000000 INFO @ Sun, 21 Jun 2020 17:57:28: 14000000 INFO @ Sun, 21 Jun 2020 17:57:29: 3000000 INFO @ Sun, 21 Jun 2020 17:57:33: 9000000 INFO @ Sun, 21 Jun 2020 17:57:34: 15000000 INFO @ Sun, 21 Jun 2020 17:57:34: 4000000 INFO @ Sun, 21 Jun 2020 17:57:39: 10000000 INFO @ Sun, 21 Jun 2020 17:57:40: 16000000 INFO @ Sun, 21 Jun 2020 17:57:40: 5000000 INFO @ Sun, 21 Jun 2020 17:57:44: 11000000 INFO @ Sun, 21 Jun 2020 17:57:46: 17000000 INFO @ Sun, 21 Jun 2020 17:57:46: 6000000 INFO @ Sun, 21 Jun 2020 17:57:50: 12000000 INFO @ Sun, 21 Jun 2020 17:57:51: 18000000 INFO @ Sun, 21 Jun 2020 17:57:52: 7000000 INFO @ Sun, 21 Jun 2020 17:57:56: 13000000 INFO @ Sun, 21 Jun 2020 17:57:57: 19000000 INFO @ Sun, 21 Jun 2020 17:57:58: 8000000 INFO @ Sun, 21 Jun 2020 17:58:02: 14000000 INFO @ Sun, 21 Jun 2020 17:58:03: 20000000 INFO @ Sun, 21 Jun 2020 17:58:03: 9000000 INFO @ Sun, 21 Jun 2020 17:58:08: 15000000 INFO @ Sun, 21 Jun 2020 17:58:09: 21000000 INFO @ Sun, 21 Jun 2020 17:58:09: 10000000 INFO @ Sun, 21 Jun 2020 17:58:13: 16000000 INFO @ Sun, 21 Jun 2020 17:58:14: 22000000 INFO @ Sun, 21 Jun 2020 17:58:15: 11000000 INFO @ Sun, 21 Jun 2020 17:58:19: 17000000 INFO @ Sun, 21 Jun 2020 17:58:20: 23000000 INFO @ Sun, 21 Jun 2020 17:58:20: 12000000 INFO @ Sun, 21 Jun 2020 17:58:25: 18000000 INFO @ Sun, 21 Jun 2020 17:58:26: 24000000 INFO @ Sun, 21 Jun 2020 17:58:26: 13000000 INFO @ Sun, 21 Jun 2020 17:58:31: 19000000 INFO @ Sun, 21 Jun 2020 17:58:32: 25000000 INFO @ Sun, 21 Jun 2020 17:58:32: 14000000 INFO @ Sun, 21 Jun 2020 17:58:36: 20000000 INFO @ Sun, 21 Jun 2020 17:58:37: 26000000 INFO @ Sun, 21 Jun 2020 17:58:38: 15000000 INFO @ Sun, 21 Jun 2020 17:58:42: 21000000 INFO @ Sun, 21 Jun 2020 17:58:43: 27000000 INFO @ Sun, 21 Jun 2020 17:58:43: 16000000 INFO @ Sun, 21 Jun 2020 17:58:46: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 17:58:46: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 17:58:46: #1 total tags in treatment: 27486395 INFO @ Sun, 21 Jun 2020 17:58:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:58:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:58:47: #1 tags after filtering in treatment: 27486395 INFO @ Sun, 21 Jun 2020 17:58:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:58:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:58:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:58:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:58:48: 22000000 INFO @ Sun, 21 Jun 2020 17:58:49: #2 number of paired peaks: 91 WARNING @ Sun, 21 Jun 2020 17:58:49: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:58:49: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 17:58:49: 17000000 cut: /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:58:53: 23000000 INFO @ Sun, 21 Jun 2020 17:58:54: 18000000 INFO @ Sun, 21 Jun 2020 17:58:59: 24000000 INFO @ Sun, 21 Jun 2020 17:58:59: 19000000 BedGraph に変換しました。 INFO @ Sun, 21 Jun 2020 17:59:04: 25000000 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:59:05: 20000000 INFO @ Sun, 21 Jun 2020 17:59:10: 26000000 INFO @ Sun, 21 Jun 2020 17:59:10: 21000000 INFO @ Sun, 21 Jun 2020 17:59:15: 27000000 INFO @ Sun, 21 Jun 2020 17:59:16: 22000000 INFO @ Sun, 21 Jun 2020 17:59:18: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 17:59:18: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 17:59:18: #1 total tags in treatment: 27486395 INFO @ Sun, 21 Jun 2020 17:59:18: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:59:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:59:19: #1 tags after filtering in treatment: 27486395 INFO @ Sun, 21 Jun 2020 17:59:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:59:19: #1 finished! INFO @ Sun, 21 Jun 2020 17:59:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:59:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:59:21: #2 number of paired peaks: 91 WARNING @ Sun, 21 Jun 2020 17:59:21: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:59:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:59:21: 23000000 INFO @ Sun, 21 Jun 2020 17:59:27: 24000000 INFO @ Sun, 21 Jun 2020 17:59:32: 25000000 INFO @ Sun, 21 Jun 2020 17:59:38: 26000000 INFO @ Sun, 21 Jun 2020 17:59:43: 27000000 INFO @ Sun, 21 Jun 2020 17:59:46: #1 tag size is determined as 40 bps INFO @ Sun, 21 Jun 2020 17:59:46: #1 tag size = 40 INFO @ Sun, 21 Jun 2020 17:59:46: #1 total tags in treatment: 27486395 INFO @ Sun, 21 Jun 2020 17:59:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:59:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:59:47: #1 tags after filtering in treatment: 27486395 INFO @ Sun, 21 Jun 2020 17:59:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:59:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:59:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:59:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:59:49: #2 number of paired peaks: 91 WARNING @ Sun, 21 Jun 2020 17:59:49: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:59:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX127436/SRX127436.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。