Job ID = 6453411 SRX = SRX113339 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:32:11 prefetch.2.10.7: 1) Downloading 'SRR392964'... 2020-06-21T08:32:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:34:27 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:34:28 prefetch.2.10.7: 'SRR392964' is valid 2020-06-21T08:34:28 prefetch.2.10.7: 1) 'SRR392964' was downloaded successfully Read 17086468 spots for SRR392964/SRR392964.sra Written 17086468 spots for SRR392964/SRR392964.sra 2020-06-21T08:35:37 prefetch.2.10.7: 1) Downloading 'SRR392965'... 2020-06-21T08:35:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:38:42 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:38:42 prefetch.2.10.7: 1) 'SRR392965' was downloaded successfully Read 21583839 spots for SRR392965/SRR392965.sra Written 21583839 spots for SRR392965/SRR392965.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:07:38 38670307 reads; of these: 38670307 (100.00%) were unpaired; of these: 7035868 (18.19%) aligned 0 times 26027633 (67.31%) aligned exactly 1 time 5606806 (14.50%) aligned >1 times 81.81% overall alignment rate Time searching: 00:07:39 Overall time: 00:07:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9470070 / 31634439 = 0.2994 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:56:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:56:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:56:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:56:47: 1000000 INFO @ Sun, 21 Jun 2020 17:56:53: 2000000 INFO @ Sun, 21 Jun 2020 17:56:59: 3000000 INFO @ Sun, 21 Jun 2020 17:57:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:57:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:57:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:57:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:57:12: 5000000 INFO @ Sun, 21 Jun 2020 17:57:18: 6000000 INFO @ Sun, 21 Jun 2020 17:57:18: 1000000 INFO @ Sun, 21 Jun 2020 17:57:25: 7000000 INFO @ Sun, 21 Jun 2020 17:57:26: 2000000 INFO @ Sun, 21 Jun 2020 17:57:31: 8000000 INFO @ Sun, 21 Jun 2020 17:57:33: 3000000 INFO @ Sun, 21 Jun 2020 17:57:37: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:57:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:57:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:57:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:57:41: 4000000 INFO @ Sun, 21 Jun 2020 17:57:44: 10000000 INFO @ Sun, 21 Jun 2020 17:57:48: 1000000 INFO @ Sun, 21 Jun 2020 17:57:49: 5000000 INFO @ Sun, 21 Jun 2020 17:57:51: 11000000 INFO @ Sun, 21 Jun 2020 17:57:55: 2000000 INFO @ Sun, 21 Jun 2020 17:57:56: 6000000 INFO @ Sun, 21 Jun 2020 17:57:58: 12000000 INFO @ Sun, 21 Jun 2020 17:58:02: 3000000 INFO @ Sun, 21 Jun 2020 17:58:04: 7000000 INFO @ Sun, 21 Jun 2020 17:58:05: 13000000 INFO @ Sun, 21 Jun 2020 17:58:09: 4000000 INFO @ Sun, 21 Jun 2020 17:58:12: 14000000 INFO @ Sun, 21 Jun 2020 17:58:12: 8000000 INFO @ Sun, 21 Jun 2020 17:58:16: 5000000 INFO @ Sun, 21 Jun 2020 17:58:19: 15000000 INFO @ Sun, 21 Jun 2020 17:58:20: 9000000 INFO @ Sun, 21 Jun 2020 17:58:23: 6000000 INFO @ Sun, 21 Jun 2020 17:58:26: 16000000 INFO @ Sun, 21 Jun 2020 17:58:27: 10000000 INFO @ Sun, 21 Jun 2020 17:58:30: 7000000 INFO @ Sun, 21 Jun 2020 17:58:33: 17000000 INFO @ Sun, 21 Jun 2020 17:58:35: 11000000 INFO @ Sun, 21 Jun 2020 17:58:37: 8000000 INFO @ Sun, 21 Jun 2020 17:58:41: 18000000 INFO @ Sun, 21 Jun 2020 17:58:42: 12000000 INFO @ Sun, 21 Jun 2020 17:58:44: 9000000 INFO @ Sun, 21 Jun 2020 17:58:48: 19000000 INFO @ Sun, 21 Jun 2020 17:58:50: 13000000 INFO @ Sun, 21 Jun 2020 17:58:51: 10000000 INFO @ Sun, 21 Jun 2020 17:58:55: 20000000 INFO @ Sun, 21 Jun 2020 17:58:57: 14000000 INFO @ Sun, 21 Jun 2020 17:58:58: 11000000 INFO @ Sun, 21 Jun 2020 17:59:02: 21000000 INFO @ Sun, 21 Jun 2020 17:59:04: 12000000 INFO @ Sun, 21 Jun 2020 17:59:04: 15000000 INFO @ Sun, 21 Jun 2020 17:59:09: 22000000 INFO @ Sun, 21 Jun 2020 17:59:10: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:59:10: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:59:10: #1 total tags in treatment: 22164369 INFO @ Sun, 21 Jun 2020 17:59:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:59:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:59:11: 13000000 INFO @ Sun, 21 Jun 2020 17:59:11: #1 tags after filtering in treatment: 22164367 INFO @ Sun, 21 Jun 2020 17:59:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:59:11: #1 finished! INFO @ Sun, 21 Jun 2020 17:59:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:59:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:59:11: 16000000 INFO @ Sun, 21 Jun 2020 17:59:12: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 17:59:12: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:59:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:59:17: 14000000 INFO @ Sun, 21 Jun 2020 17:59:19: 17000000 INFO @ Sun, 21 Jun 2020 17:59:22: 15000000 INFO @ Sun, 21 Jun 2020 17:59:26: 18000000 INFO @ Sun, 21 Jun 2020 17:59:28: 16000000 INFO @ Sun, 21 Jun 2020 17:59:34: 19000000 INFO @ Sun, 21 Jun 2020 17:59:34: 17000000 INFO @ Sun, 21 Jun 2020 17:59:40: 18000000 INFO @ Sun, 21 Jun 2020 17:59:41: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:59:47: 19000000 INFO @ Sun, 21 Jun 2020 17:59:47: 21000000 INFO @ Sun, 21 Jun 2020 17:59:52: 20000000 INFO @ Sun, 21 Jun 2020 17:59:55: 22000000 INFO @ Sun, 21 Jun 2020 17:59:56: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:59:56: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:59:56: #1 total tags in treatment: 22164369 INFO @ Sun, 21 Jun 2020 17:59:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:59:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:59:57: #1 tags after filtering in treatment: 22164367 INFO @ Sun, 21 Jun 2020 17:59:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:59:57: #1 finished! INFO @ Sun, 21 Jun 2020 17:59:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:59:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:59:58: 21000000 INFO @ Sun, 21 Jun 2020 17:59:58: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 17:59:58: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:59:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:00:04: 22000000 INFO @ Sun, 21 Jun 2020 18:00:06: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 18:00:06: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 18:00:06: #1 total tags in treatment: 22164369 INFO @ Sun, 21 Jun 2020 18:00:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:00:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:00:06: #1 tags after filtering in treatment: 22164367 INFO @ Sun, 21 Jun 2020 18:00:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:00:06: #1 finished! INFO @ Sun, 21 Jun 2020 18:00:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:00:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:00:08: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 18:00:08: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:00:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113339/SRX113339.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。