Job ID = 6453408 SRX = SRX113336 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:15:03 prefetch.2.10.7: 1) Downloading 'SRR392959'... 2020-06-21T08:15:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:19:25 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:19:25 prefetch.2.10.7: 1) 'SRR392959' was downloaded successfully Read 20576294 spots for SRR392959/SRR392959.sra Written 20576294 spots for SRR392959/SRR392959.sra 2020-06-21T08:20:40 prefetch.2.10.7: 1) Downloading 'SRR392960'... 2020-06-21T08:20:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:23:33 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:23:34 prefetch.2.10.7: 'SRR392960' is valid 2020-06-21T08:23:34 prefetch.2.10.7: 1) 'SRR392960' was downloaded successfully Read 17170791 spots for SRR392960/SRR392960.sra Written 17170791 spots for SRR392960/SRR392960.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:17 37747085 reads; of these: 37747085 (100.00%) were unpaired; of these: 3180185 (8.42%) aligned 0 times 28783414 (76.25%) aligned exactly 1 time 5783486 (15.32%) aligned >1 times 91.58% overall alignment rate Time searching: 00:07:17 Overall time: 00:07:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12943011 / 34566900 = 0.3744 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:39:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:39:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:39:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:39:16: 1000000 INFO @ Sun, 21 Jun 2020 17:39:23: 2000000 INFO @ Sun, 21 Jun 2020 17:39:30: 3000000 INFO @ Sun, 21 Jun 2020 17:39:37: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:39:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:39:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:39:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:39:44: 5000000 INFO @ Sun, 21 Jun 2020 17:39:47: 1000000 INFO @ Sun, 21 Jun 2020 17:39:51: 6000000 INFO @ Sun, 21 Jun 2020 17:39:54: 2000000 INFO @ Sun, 21 Jun 2020 17:39:58: 7000000 INFO @ Sun, 21 Jun 2020 17:40:02: 3000000 INFO @ Sun, 21 Jun 2020 17:40:06: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:40:09: 4000000 INFO @ Sun, 21 Jun 2020 17:40:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:40:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:40:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:40:14: 9000000 INFO @ Sun, 21 Jun 2020 17:40:16: 1000000 INFO @ Sun, 21 Jun 2020 17:40:17: 5000000 INFO @ Sun, 21 Jun 2020 17:40:21: 10000000 INFO @ Sun, 21 Jun 2020 17:40:23: 2000000 INFO @ Sun, 21 Jun 2020 17:40:24: 6000000 INFO @ Sun, 21 Jun 2020 17:40:29: 11000000 INFO @ Sun, 21 Jun 2020 17:40:30: 3000000 INFO @ Sun, 21 Jun 2020 17:40:32: 7000000 INFO @ Sun, 21 Jun 2020 17:40:36: 4000000 INFO @ Sun, 21 Jun 2020 17:40:36: 12000000 INFO @ Sun, 21 Jun 2020 17:40:39: 8000000 INFO @ Sun, 21 Jun 2020 17:40:43: 5000000 INFO @ Sun, 21 Jun 2020 17:40:44: 13000000 INFO @ Sun, 21 Jun 2020 17:40:47: 9000000 INFO @ Sun, 21 Jun 2020 17:40:50: 6000000 INFO @ Sun, 21 Jun 2020 17:40:52: 14000000 INFO @ Sun, 21 Jun 2020 17:40:55: 10000000 INFO @ Sun, 21 Jun 2020 17:40:56: 7000000 INFO @ Sun, 21 Jun 2020 17:41:00: 15000000 INFO @ Sun, 21 Jun 2020 17:41:02: 11000000 INFO @ Sun, 21 Jun 2020 17:41:03: 8000000 INFO @ Sun, 21 Jun 2020 17:41:07: 16000000 INFO @ Sun, 21 Jun 2020 17:41:10: 9000000 INFO @ Sun, 21 Jun 2020 17:41:10: 12000000 INFO @ Sun, 21 Jun 2020 17:41:15: 17000000 INFO @ Sun, 21 Jun 2020 17:41:16: 10000000 INFO @ Sun, 21 Jun 2020 17:41:18: 13000000 INFO @ Sun, 21 Jun 2020 17:41:23: 18000000 INFO @ Sun, 21 Jun 2020 17:41:23: 11000000 INFO @ Sun, 21 Jun 2020 17:41:25: 14000000 INFO @ Sun, 21 Jun 2020 17:41:30: 12000000 INFO @ Sun, 21 Jun 2020 17:41:31: 19000000 INFO @ Sun, 21 Jun 2020 17:41:33: 15000000 INFO @ Sun, 21 Jun 2020 17:41:37: 13000000 INFO @ Sun, 21 Jun 2020 17:41:38: 20000000 INFO @ Sun, 21 Jun 2020 17:41:40: 16000000 INFO @ Sun, 21 Jun 2020 17:41:43: 14000000 INFO @ Sun, 21 Jun 2020 17:41:46: 21000000 INFO @ Sun, 21 Jun 2020 17:41:48: 17000000 INFO @ Sun, 21 Jun 2020 17:41:50: 15000000 INFO @ Sun, 21 Jun 2020 17:41:50: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:41:50: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:41:50: #1 total tags in treatment: 21623889 INFO @ Sun, 21 Jun 2020 17:41:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:41:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:41:51: #1 tags after filtering in treatment: 21623889 INFO @ Sun, 21 Jun 2020 17:41:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:41:51: #1 finished! INFO @ Sun, 21 Jun 2020 17:41:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:41:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:41:52: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 17:41:52: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:41:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:41:56: 18000000 INFO @ Sun, 21 Jun 2020 17:41:57: 16000000 INFO @ Sun, 21 Jun 2020 17:42:03: 17000000 INFO @ Sun, 21 Jun 2020 17:42:04: 19000000 INFO @ Sun, 21 Jun 2020 17:42:11: 18000000 INFO @ Sun, 21 Jun 2020 17:42:11: 20000000 INFO @ Sun, 21 Jun 2020 17:42:17: 19000000 INFO @ Sun, 21 Jun 2020 17:42:19: 21000000 INFO @ Sun, 21 Jun 2020 17:42:23: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:42:23: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:42:23: #1 total tags in treatment: 21623889 INFO @ Sun, 21 Jun 2020 17:42:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:42:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:42:24: 20000000 INFO @ Sun, 21 Jun 2020 17:42:24: #1 tags after filtering in treatment: 21623889 INFO @ Sun, 21 Jun 2020 17:42:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:42:24: #1 finished! INFO @ Sun, 21 Jun 2020 17:42:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:42:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:42:25: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 17:42:25: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:42:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:42:30: 21000000 INFO @ Sun, 21 Jun 2020 17:42:34: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:42:34: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:42:34: #1 total tags in treatment: 21623889 INFO @ Sun, 21 Jun 2020 17:42:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:42:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:42:35: #1 tags after filtering in treatment: 21623889 INFO @ Sun, 21 Jun 2020 17:42:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:42:35: #1 finished! INFO @ Sun, 21 Jun 2020 17:42:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:42:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:42:36: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 17:42:36: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:42:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113336/SRX113336.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。