Job ID = 6453407 SRX = SRX113335 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:24:02 prefetch.2.10.7: 1) Downloading 'SRR392958'... 2020-06-21T08:24:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:25:55 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:25:55 prefetch.2.10.7: 1) 'SRR392958' was downloaded successfully Read 13655555 spots for SRR392958/SRR392958.sra Written 13655555 spots for SRR392958/SRR392958.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 13655555 reads; of these: 13655555 (100.00%) were unpaired; of these: 1388207 (10.17%) aligned 0 times 10555232 (77.30%) aligned exactly 1 time 1712116 (12.54%) aligned >1 times 89.83% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2647699 / 12267348 = 0.2158 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:33:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:33:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:33:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:33:11: 1000000 INFO @ Sun, 21 Jun 2020 17:33:18: 2000000 INFO @ Sun, 21 Jun 2020 17:33:25: 3000000 INFO @ Sun, 21 Jun 2020 17:33:32: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:33:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:33:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:33:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:33:40: 5000000 INFO @ Sun, 21 Jun 2020 17:33:42: 1000000 INFO @ Sun, 21 Jun 2020 17:33:47: 6000000 INFO @ Sun, 21 Jun 2020 17:33:49: 2000000 INFO @ Sun, 21 Jun 2020 17:33:55: 7000000 INFO @ Sun, 21 Jun 2020 17:33:57: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:34:03: 8000000 INFO @ Sun, 21 Jun 2020 17:34:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:34:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:34:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:34:04: 4000000 INFO @ Sun, 21 Jun 2020 17:34:11: 9000000 INFO @ Sun, 21 Jun 2020 17:34:12: 1000000 INFO @ Sun, 21 Jun 2020 17:34:12: 5000000 INFO @ Sun, 21 Jun 2020 17:34:17: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:34:17: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:34:17: #1 total tags in treatment: 9619649 INFO @ Sun, 21 Jun 2020 17:34:17: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:34:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:34:17: #1 tags after filtering in treatment: 9619636 INFO @ Sun, 21 Jun 2020 17:34:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:34:17: #1 finished! INFO @ Sun, 21 Jun 2020 17:34:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:34:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:34:18: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 17:34:18: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:34:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:34:19: 2000000 INFO @ Sun, 21 Jun 2020 17:34:20: 6000000 INFO @ Sun, 21 Jun 2020 17:34:27: 3000000 INFO @ Sun, 21 Jun 2020 17:34:28: 7000000 INFO @ Sun, 21 Jun 2020 17:34:34: 4000000 INFO @ Sun, 21 Jun 2020 17:34:36: 8000000 INFO @ Sun, 21 Jun 2020 17:34:41: 5000000 INFO @ Sun, 21 Jun 2020 17:34:45: 9000000 INFO @ Sun, 21 Jun 2020 17:34:49: 6000000 INFO @ Sun, 21 Jun 2020 17:34:50: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:34:50: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:34:50: #1 total tags in treatment: 9619649 INFO @ Sun, 21 Jun 2020 17:34:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:34:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:34:51: #1 tags after filtering in treatment: 9619636 INFO @ Sun, 21 Jun 2020 17:34:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:34:51: #1 finished! INFO @ Sun, 21 Jun 2020 17:34:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:34:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:34:51: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 17:34:51: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:34:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:34:56: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:35:02: 8000000 INFO @ Sun, 21 Jun 2020 17:35:09: 9000000 INFO @ Sun, 21 Jun 2020 17:35:13: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:35:13: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:35:13: #1 total tags in treatment: 9619649 INFO @ Sun, 21 Jun 2020 17:35:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:35:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:35:13: #1 tags after filtering in treatment: 9619636 INFO @ Sun, 21 Jun 2020 17:35:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:35:13: #1 finished! INFO @ Sun, 21 Jun 2020 17:35:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:35:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:35:14: #2 number of paired peaks: 39 WARNING @ Sun, 21 Jun 2020 17:35:14: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:35:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113335/SRX113335.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。