Job ID = 6453406 SRX = SRX113334 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:29:26 prefetch.2.10.7: 1) Downloading 'SRR392957'... 2020-06-21T08:29:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:33:36 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:33:36 prefetch.2.10.7: 1) 'SRR392957' was downloaded successfully Read 22749432 spots for SRR392957/SRR392957.sra Written 22749432 spots for SRR392957/SRR392957.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:17 22749432 reads; of these: 22749432 (100.00%) were unpaired; of these: 1041381 (4.58%) aligned 0 times 17937096 (78.85%) aligned exactly 1 time 3770955 (16.58%) aligned >1 times 95.42% overall alignment rate Time searching: 00:05:18 Overall time: 00:05:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4104445 / 21708051 = 0.1891 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:46:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:46:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:46:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:47:06: 1000000 INFO @ Sun, 21 Jun 2020 17:47:12: 2000000 INFO @ Sun, 21 Jun 2020 17:47:19: 3000000 INFO @ Sun, 21 Jun 2020 17:47:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:47:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:47:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:47:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:47:33: 5000000 INFO @ Sun, 21 Jun 2020 17:47:37: 1000000 INFO @ Sun, 21 Jun 2020 17:47:40: 6000000 INFO @ Sun, 21 Jun 2020 17:47:44: 2000000 INFO @ Sun, 21 Jun 2020 17:47:47: 7000000 INFO @ Sun, 21 Jun 2020 17:47:51: 3000000 INFO @ Sun, 21 Jun 2020 17:47:55: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:47:59: 4000000 INFO @ Sun, 21 Jun 2020 17:47:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:47:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:47:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:48:02: 9000000 INFO @ Sun, 21 Jun 2020 17:48:07: 5000000 INFO @ Sun, 21 Jun 2020 17:48:07: 1000000 INFO @ Sun, 21 Jun 2020 17:48:10: 10000000 INFO @ Sun, 21 Jun 2020 17:48:15: 6000000 INFO @ Sun, 21 Jun 2020 17:48:15: 2000000 INFO @ Sun, 21 Jun 2020 17:48:18: 11000000 INFO @ Sun, 21 Jun 2020 17:48:23: 7000000 INFO @ Sun, 21 Jun 2020 17:48:23: 3000000 INFO @ Sun, 21 Jun 2020 17:48:26: 12000000 INFO @ Sun, 21 Jun 2020 17:48:31: 8000000 INFO @ Sun, 21 Jun 2020 17:48:31: 4000000 INFO @ Sun, 21 Jun 2020 17:48:34: 13000000 INFO @ Sun, 21 Jun 2020 17:48:39: 9000000 INFO @ Sun, 21 Jun 2020 17:48:39: 5000000 INFO @ Sun, 21 Jun 2020 17:48:42: 14000000 INFO @ Sun, 21 Jun 2020 17:48:47: 6000000 INFO @ Sun, 21 Jun 2020 17:48:47: 10000000 INFO @ Sun, 21 Jun 2020 17:48:50: 15000000 INFO @ Sun, 21 Jun 2020 17:48:54: 7000000 INFO @ Sun, 21 Jun 2020 17:48:55: 11000000 INFO @ Sun, 21 Jun 2020 17:48:58: 16000000 INFO @ Sun, 21 Jun 2020 17:49:02: 8000000 INFO @ Sun, 21 Jun 2020 17:49:03: 12000000 INFO @ Sun, 21 Jun 2020 17:49:06: 17000000 INFO @ Sun, 21 Jun 2020 17:49:10: 9000000 INFO @ Sun, 21 Jun 2020 17:49:10: 13000000 INFO @ Sun, 21 Jun 2020 17:49:11: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:49:11: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:49:11: #1 total tags in treatment: 17603606 INFO @ Sun, 21 Jun 2020 17:49:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:49:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:49:12: #1 tags after filtering in treatment: 17603604 INFO @ Sun, 21 Jun 2020 17:49:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:49:12: #1 finished! INFO @ Sun, 21 Jun 2020 17:49:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:49:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:49:13: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 17:49:13: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:49:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:49:18: 10000000 INFO @ Sun, 21 Jun 2020 17:49:18: 14000000 INFO @ Sun, 21 Jun 2020 17:49:25: 11000000 INFO @ Sun, 21 Jun 2020 17:49:26: 15000000 INFO @ Sun, 21 Jun 2020 17:49:33: 12000000 INFO @ Sun, 21 Jun 2020 17:49:34: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:49:40: 13000000 INFO @ Sun, 21 Jun 2020 17:49:42: 17000000 INFO @ Sun, 21 Jun 2020 17:49:47: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:49:47: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:49:47: #1 total tags in treatment: 17603606 INFO @ Sun, 21 Jun 2020 17:49:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:49:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:49:47: 14000000 INFO @ Sun, 21 Jun 2020 17:49:47: #1 tags after filtering in treatment: 17603604 INFO @ Sun, 21 Jun 2020 17:49:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:49:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:49:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:49:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:49:49: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 17:49:49: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:49:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:49:55: 15000000 INFO @ Sun, 21 Jun 2020 17:50:02: 16000000 INFO @ Sun, 21 Jun 2020 17:50:09: 17000000 INFO @ Sun, 21 Jun 2020 17:50:13: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:50:13: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:50:13: #1 total tags in treatment: 17603606 INFO @ Sun, 21 Jun 2020 17:50:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:50:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:50:14: #1 tags after filtering in treatment: 17603604 INFO @ Sun, 21 Jun 2020 17:50:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:50:14: #1 finished! INFO @ Sun, 21 Jun 2020 17:50:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:50:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:50:15: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 17:50:15: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:50:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113334/SRX113334.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。