Job ID = 6453401 SRX = SRX113329 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:12:03 prefetch.2.10.7: 1) Downloading 'SRR392949'... 2020-06-21T08:12:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:17:17 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:17:17 prefetch.2.10.7: 1) 'SRR392949' was downloaded successfully Read 30861048 spots for SRR392949/SRR392949.sra Written 30861048 spots for SRR392949/SRR392949.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:58 30861048 reads; of these: 30861048 (100.00%) were unpaired; of these: 3668155 (11.89%) aligned 0 times 22468091 (72.80%) aligned exactly 1 time 4724802 (15.31%) aligned >1 times 88.11% overall alignment rate Time searching: 00:07:58 Overall time: 00:07:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6007109 / 27192893 = 0.2209 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:33:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:33:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:33:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:34:03: 1000000 INFO @ Sun, 21 Jun 2020 17:34:08: 2000000 INFO @ Sun, 21 Jun 2020 17:34:13: 3000000 INFO @ Sun, 21 Jun 2020 17:34:18: 4000000 INFO @ Sun, 21 Jun 2020 17:34:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:34:28: 6000000 INFO @ Sun, 21 Jun 2020 17:34:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:34:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:34:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:34:33: 7000000 INFO @ Sun, 21 Jun 2020 17:34:34: 1000000 INFO @ Sun, 21 Jun 2020 17:34:39: 8000000 INFO @ Sun, 21 Jun 2020 17:34:39: 2000000 INFO @ Sun, 21 Jun 2020 17:34:44: 9000000 INFO @ Sun, 21 Jun 2020 17:34:45: 3000000 INFO @ Sun, 21 Jun 2020 17:34:49: 10000000 INFO @ Sun, 21 Jun 2020 17:34:50: 4000000 INFO @ Sun, 21 Jun 2020 17:34:54: 11000000 INFO @ Sun, 21 Jun 2020 17:34:55: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:34:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:34:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:34:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:35:00: 12000000 INFO @ Sun, 21 Jun 2020 17:35:01: 6000000 INFO @ Sun, 21 Jun 2020 17:35:03: 1000000 INFO @ Sun, 21 Jun 2020 17:35:05: 13000000 INFO @ Sun, 21 Jun 2020 17:35:06: 7000000 INFO @ Sun, 21 Jun 2020 17:35:09: 2000000 INFO @ Sun, 21 Jun 2020 17:35:10: 14000000 INFO @ Sun, 21 Jun 2020 17:35:11: 8000000 INFO @ Sun, 21 Jun 2020 17:35:14: 3000000 INFO @ Sun, 21 Jun 2020 17:35:16: 15000000 INFO @ Sun, 21 Jun 2020 17:35:17: 9000000 INFO @ Sun, 21 Jun 2020 17:35:19: 4000000 INFO @ Sun, 21 Jun 2020 17:35:21: 16000000 INFO @ Sun, 21 Jun 2020 17:35:22: 10000000 INFO @ Sun, 21 Jun 2020 17:35:25: 5000000 INFO @ Sun, 21 Jun 2020 17:35:26: 17000000 INFO @ Sun, 21 Jun 2020 17:35:27: 11000000 INFO @ Sun, 21 Jun 2020 17:35:30: 6000000 INFO @ Sun, 21 Jun 2020 17:35:32: 18000000 INFO @ Sun, 21 Jun 2020 17:35:33: 12000000 INFO @ Sun, 21 Jun 2020 17:35:35: 7000000 INFO @ Sun, 21 Jun 2020 17:35:37: 19000000 INFO @ Sun, 21 Jun 2020 17:35:38: 13000000 INFO @ Sun, 21 Jun 2020 17:35:41: 8000000 INFO @ Sun, 21 Jun 2020 17:35:43: 20000000 INFO @ Sun, 21 Jun 2020 17:35:43: 14000000 INFO @ Sun, 21 Jun 2020 17:35:46: 9000000 INFO @ Sun, 21 Jun 2020 17:35:48: 21000000 INFO @ Sun, 21 Jun 2020 17:35:49: 15000000 INFO @ Sun, 21 Jun 2020 17:35:49: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:35:49: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:35:49: #1 total tags in treatment: 21185784 INFO @ Sun, 21 Jun 2020 17:35:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:35:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:35:50: #1 tags after filtering in treatment: 21185781 INFO @ Sun, 21 Jun 2020 17:35:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:35:50: #1 finished! INFO @ Sun, 21 Jun 2020 17:35:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:35:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:35:51: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 17:35:51: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:35:51: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 17:35:52: 10000000 cut: /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:35:54: 16000000 INFO @ Sun, 21 Jun 2020 17:35:57: 11000000 INFO @ Sun, 21 Jun 2020 17:35:59: 17000000 INFO @ Sun, 21 Jun 2020 17:36:02: 12000000 INFO @ Sun, 21 Jun 2020 17:36:05: 18000000 INFO @ Sun, 21 Jun 2020 17:36:07: 13000000 INFO @ Sun, 21 Jun 2020 17:36:10: 19000000 INFO @ Sun, 21 Jun 2020 17:36:13: 14000000 INFO @ Sun, 21 Jun 2020 17:36:15: 20000000 INFO @ Sun, 21 Jun 2020 17:36:18: 15000000 INFO @ Sun, 21 Jun 2020 17:36:21: 21000000 INFO @ Sun, 21 Jun 2020 17:36:22: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:36:22: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:36:22: #1 total tags in treatment: 21185784 INFO @ Sun, 21 Jun 2020 17:36:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:36:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:36:23: #1 tags after filtering in treatment: 21185781 INFO @ Sun, 21 Jun 2020 17:36:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:36:23: #1 finished! INFO @ Sun, 21 Jun 2020 17:36:23: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:36:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:36:23: 16000000 INFO @ Sun, 21 Jun 2020 17:36:24: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 17:36:24: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:36:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:36:28: 17000000 INFO @ Sun, 21 Jun 2020 17:36:34: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:36:39: 19000000 INFO @ Sun, 21 Jun 2020 17:36:44: 20000000 INFO @ Sun, 21 Jun 2020 17:36:49: 21000000 INFO @ Sun, 21 Jun 2020 17:36:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:36:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:36:51: #1 total tags in treatment: 21185784 INFO @ Sun, 21 Jun 2020 17:36:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:36:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:36:51: #1 tags after filtering in treatment: 21185781 INFO @ Sun, 21 Jun 2020 17:36:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:36:51: #1 finished! INFO @ Sun, 21 Jun 2020 17:36:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:36:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:36:52: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 17:36:52: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:36:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113329/SRX113329.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。