Job ID = 6453393 SRX = SRX113323 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:15:48 prefetch.2.10.7: 1) Downloading 'SRR392938'... 2020-06-21T08:15:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:18:35 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:18:35 prefetch.2.10.7: 1) 'SRR392938' was downloaded successfully Read 20069041 spots for SRR392938/SRR392938.sra Written 20069041 spots for SRR392938/SRR392938.sra 2020-06-21T08:19:41 prefetch.2.10.7: 1) Downloading 'SRR392939'... 2020-06-21T08:19:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:22:54 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:22:54 prefetch.2.10.7: 1) 'SRR392939' was downloaded successfully Read 21266162 spots for SRR392939/SRR392939.sra Written 21266162 spots for SRR392939/SRR392939.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:07 41335203 reads; of these: 41335203 (100.00%) were unpaired; of these: 2880847 (6.97%) aligned 0 times 28188303 (68.19%) aligned exactly 1 time 10266053 (24.84%) aligned >1 times 93.03% overall alignment rate Time searching: 00:09:07 Overall time: 00:09:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14694860 / 38454356 = 0.3821 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:41:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:41:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:41:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:41:16: 1000000 INFO @ Sun, 21 Jun 2020 17:41:21: 2000000 INFO @ Sun, 21 Jun 2020 17:41:27: 3000000 INFO @ Sun, 21 Jun 2020 17:41:32: 4000000 INFO @ Sun, 21 Jun 2020 17:41:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:41:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:41:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:41:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:41:42: 6000000 INFO @ Sun, 21 Jun 2020 17:41:47: 1000000 INFO @ Sun, 21 Jun 2020 17:41:48: 7000000 INFO @ Sun, 21 Jun 2020 17:41:53: 2000000 INFO @ Sun, 21 Jun 2020 17:41:54: 8000000 INFO @ Sun, 21 Jun 2020 17:41:59: 3000000 INFO @ Sun, 21 Jun 2020 17:42:00: 9000000 INFO @ Sun, 21 Jun 2020 17:42:04: 4000000 INFO @ Sun, 21 Jun 2020 17:42:06: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:42:10: 5000000 INFO @ Sun, 21 Jun 2020 17:42:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:42:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:42:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:42:11: 11000000 INFO @ Sun, 21 Jun 2020 17:42:16: 6000000 INFO @ Sun, 21 Jun 2020 17:42:17: 1000000 INFO @ Sun, 21 Jun 2020 17:42:17: 12000000 INFO @ Sun, 21 Jun 2020 17:42:22: 7000000 INFO @ Sun, 21 Jun 2020 17:42:23: 2000000 INFO @ Sun, 21 Jun 2020 17:42:23: 13000000 INFO @ Sun, 21 Jun 2020 17:42:28: 8000000 INFO @ Sun, 21 Jun 2020 17:42:29: 3000000 INFO @ Sun, 21 Jun 2020 17:42:29: 14000000 INFO @ Sun, 21 Jun 2020 17:42:34: 9000000 INFO @ Sun, 21 Jun 2020 17:42:35: 4000000 INFO @ Sun, 21 Jun 2020 17:42:35: 15000000 INFO @ Sun, 21 Jun 2020 17:42:39: 10000000 INFO @ Sun, 21 Jun 2020 17:42:41: 5000000 INFO @ Sun, 21 Jun 2020 17:42:41: 16000000 INFO @ Sun, 21 Jun 2020 17:42:45: 11000000 INFO @ Sun, 21 Jun 2020 17:42:47: 6000000 INFO @ Sun, 21 Jun 2020 17:42:47: 17000000 INFO @ Sun, 21 Jun 2020 17:42:51: 12000000 INFO @ Sun, 21 Jun 2020 17:42:53: 7000000 INFO @ Sun, 21 Jun 2020 17:42:53: 18000000 INFO @ Sun, 21 Jun 2020 17:42:57: 13000000 INFO @ Sun, 21 Jun 2020 17:42:59: 8000000 INFO @ Sun, 21 Jun 2020 17:42:59: 19000000 INFO @ Sun, 21 Jun 2020 17:43:03: 14000000 INFO @ Sun, 21 Jun 2020 17:43:05: 9000000 INFO @ Sun, 21 Jun 2020 17:43:05: 20000000 INFO @ Sun, 21 Jun 2020 17:43:09: 15000000 INFO @ Sun, 21 Jun 2020 17:43:11: 10000000 INFO @ Sun, 21 Jun 2020 17:43:11: 21000000 INFO @ Sun, 21 Jun 2020 17:43:14: 16000000 INFO @ Sun, 21 Jun 2020 17:43:17: 22000000 INFO @ Sun, 21 Jun 2020 17:43:17: 11000000 INFO @ Sun, 21 Jun 2020 17:43:20: 17000000 INFO @ Sun, 21 Jun 2020 17:43:23: 23000000 INFO @ Sun, 21 Jun 2020 17:43:23: 12000000 INFO @ Sun, 21 Jun 2020 17:43:26: 18000000 INFO @ Sun, 21 Jun 2020 17:43:27: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:43:27: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:43:27: #1 total tags in treatment: 23759496 INFO @ Sun, 21 Jun 2020 17:43:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:43:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:43:28: #1 tags after filtering in treatment: 23759446 INFO @ Sun, 21 Jun 2020 17:43:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:43:28: #1 finished! INFO @ Sun, 21 Jun 2020 17:43:28: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:43:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:43:29: 13000000 INFO @ Sun, 21 Jun 2020 17:43:29: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 17:43:29: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:43:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:43:32: 19000000 INFO @ Sun, 21 Jun 2020 17:43:34: 14000000 INFO @ Sun, 21 Jun 2020 17:43:38: 20000000 INFO @ Sun, 21 Jun 2020 17:43:40: 15000000 INFO @ Sun, 21 Jun 2020 17:43:44: 21000000 INFO @ Sun, 21 Jun 2020 17:43:46: 16000000 INFO @ Sun, 21 Jun 2020 17:43:50: 22000000 INFO @ Sun, 21 Jun 2020 17:43:52: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:43:55: 23000000 INFO @ Sun, 21 Jun 2020 17:43:58: 18000000 INFO @ Sun, 21 Jun 2020 17:44:00: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:44:00: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:44:00: #1 total tags in treatment: 23759496 INFO @ Sun, 21 Jun 2020 17:44:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:44:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:44:01: #1 tags after filtering in treatment: 23759446 INFO @ Sun, 21 Jun 2020 17:44:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:44:01: #1 finished! INFO @ Sun, 21 Jun 2020 17:44:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:44:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:44:02: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 17:44:02: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:44:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:44:04: 19000000 INFO @ Sun, 21 Jun 2020 17:44:09: 20000000 INFO @ Sun, 21 Jun 2020 17:44:14: 21000000 INFO @ Sun, 21 Jun 2020 17:44:20: 22000000 INFO @ Sun, 21 Jun 2020 17:44:25: 23000000 INFO @ Sun, 21 Jun 2020 17:44:29: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:44:29: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:44:29: #1 total tags in treatment: 23759496 INFO @ Sun, 21 Jun 2020 17:44:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:44:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:44:30: #1 tags after filtering in treatment: 23759446 INFO @ Sun, 21 Jun 2020 17:44:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:44:30: #1 finished! INFO @ Sun, 21 Jun 2020 17:44:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:44:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:44:31: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 17:44:31: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:44:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113323/SRX113323.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。