Job ID = 6453392 SRX = SRX113319 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:15:33 prefetch.2.10.7: 1) Downloading 'SRR392931'... 2020-06-21T08:15:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:18:23 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:18:23 prefetch.2.10.7: 1) 'SRR392931' was downloaded successfully Read 19804394 spots for SRR392931/SRR392931.sra Written 19804394 spots for SRR392931/SRR392931.sra 2020-06-21T08:19:27 prefetch.2.10.7: 1) Downloading 'SRR392932'... 2020-06-21T08:19:27 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:21:56 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:21:57 prefetch.2.10.7: 'SRR392932' is valid 2020-06-21T08:21:57 prefetch.2.10.7: 1) 'SRR392932' was downloaded successfully Read 17014202 spots for SRR392932/SRR392932.sra Written 17014202 spots for SRR392932/SRR392932.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:45 36818596 reads; of these: 36818596 (100.00%) were unpaired; of these: 701466 (1.91%) aligned 0 times 26345645 (71.56%) aligned exactly 1 time 9771485 (26.54%) aligned >1 times 98.09% overall alignment rate Time searching: 00:08:45 Overall time: 00:08:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13819804 / 36117130 = 0.3826 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:39:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:39:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:39:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:39:18: 1000000 INFO @ Sun, 21 Jun 2020 17:39:24: 2000000 INFO @ Sun, 21 Jun 2020 17:39:30: 3000000 INFO @ Sun, 21 Jun 2020 17:39:36: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:39:41: 5000000 INFO @ Sun, 21 Jun 2020 17:39:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:39:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:39:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:39:47: 1000000 INFO @ Sun, 21 Jun 2020 17:39:47: 6000000 INFO @ Sun, 21 Jun 2020 17:39:53: 2000000 INFO @ Sun, 21 Jun 2020 17:39:54: 7000000 INFO @ Sun, 21 Jun 2020 17:39:59: 3000000 INFO @ Sun, 21 Jun 2020 17:40:00: 8000000 INFO @ Sun, 21 Jun 2020 17:40:04: 4000000 INFO @ Sun, 21 Jun 2020 17:40:06: 9000000 INFO @ Sun, 21 Jun 2020 17:40:10: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:40:12: 10000000 INFO @ Sun, 21 Jun 2020 17:40:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:40:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:40:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:40:15: 6000000 INFO @ Sun, 21 Jun 2020 17:40:18: 1000000 INFO @ Sun, 21 Jun 2020 17:40:18: 11000000 INFO @ Sun, 21 Jun 2020 17:40:21: 7000000 INFO @ Sun, 21 Jun 2020 17:40:23: 2000000 INFO @ Sun, 21 Jun 2020 17:40:24: 12000000 INFO @ Sun, 21 Jun 2020 17:40:26: 8000000 INFO @ Sun, 21 Jun 2020 17:40:29: 3000000 INFO @ Sun, 21 Jun 2020 17:40:30: 13000000 INFO @ Sun, 21 Jun 2020 17:40:32: 9000000 INFO @ Sun, 21 Jun 2020 17:40:34: 4000000 INFO @ Sun, 21 Jun 2020 17:40:36: 14000000 INFO @ Sun, 21 Jun 2020 17:40:37: 10000000 INFO @ Sun, 21 Jun 2020 17:40:40: 5000000 INFO @ Sun, 21 Jun 2020 17:40:42: 15000000 INFO @ Sun, 21 Jun 2020 17:40:43: 11000000 INFO @ Sun, 21 Jun 2020 17:40:45: 6000000 INFO @ Sun, 21 Jun 2020 17:40:48: 16000000 INFO @ Sun, 21 Jun 2020 17:40:48: 12000000 INFO @ Sun, 21 Jun 2020 17:40:51: 7000000 INFO @ Sun, 21 Jun 2020 17:40:54: 17000000 INFO @ Sun, 21 Jun 2020 17:40:54: 13000000 INFO @ Sun, 21 Jun 2020 17:40:56: 8000000 INFO @ Sun, 21 Jun 2020 17:40:59: 14000000 INFO @ Sun, 21 Jun 2020 17:41:00: 18000000 INFO @ Sun, 21 Jun 2020 17:41:02: 9000000 INFO @ Sun, 21 Jun 2020 17:41:05: 15000000 INFO @ Sun, 21 Jun 2020 17:41:06: 19000000 INFO @ Sun, 21 Jun 2020 17:41:07: 10000000 INFO @ Sun, 21 Jun 2020 17:41:10: 16000000 INFO @ Sun, 21 Jun 2020 17:41:12: 20000000 INFO @ Sun, 21 Jun 2020 17:41:13: 11000000 INFO @ Sun, 21 Jun 2020 17:41:16: 17000000 INFO @ Sun, 21 Jun 2020 17:41:18: 12000000 INFO @ Sun, 21 Jun 2020 17:41:18: 21000000 INFO @ Sun, 21 Jun 2020 17:41:21: 18000000 INFO @ Sun, 21 Jun 2020 17:41:24: 13000000 INFO @ Sun, 21 Jun 2020 17:41:24: 22000000 INFO @ Sun, 21 Jun 2020 17:41:27: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:41:27: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:41:27: #1 total tags in treatment: 22297326 INFO @ Sun, 21 Jun 2020 17:41:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:41:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:41:27: 19000000 INFO @ Sun, 21 Jun 2020 17:41:27: #1 tags after filtering in treatment: 22297326 INFO @ Sun, 21 Jun 2020 17:41:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:41:27: #1 finished! INFO @ Sun, 21 Jun 2020 17:41:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:41:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:41:28: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 17:41:28: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:41:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:41:29: 14000000 INFO @ Sun, 21 Jun 2020 17:41:32: 20000000 INFO @ Sun, 21 Jun 2020 17:41:34: 15000000 INFO @ Sun, 21 Jun 2020 17:41:38: 21000000 INFO @ Sun, 21 Jun 2020 17:41:39: 16000000 INFO @ Sun, 21 Jun 2020 17:41:43: 22000000 INFO @ Sun, 21 Jun 2020 17:41:45: 17000000 INFO @ Sun, 21 Jun 2020 17:41:45: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:41:45: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:41:45: #1 total tags in treatment: 22297326 INFO @ Sun, 21 Jun 2020 17:41:45: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:41:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:41:46: #1 tags after filtering in treatment: 22297326 INFO @ Sun, 21 Jun 2020 17:41:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:41:46: #1 finished! INFO @ Sun, 21 Jun 2020 17:41:46: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:41:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:41:47: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 17:41:47: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:41:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:41:50: 18000000 INFO @ Sun, 21 Jun 2020 17:41:55: 19000000 INFO @ Sun, 21 Jun 2020 17:42:00: 20000000 INFO @ Sun, 21 Jun 2020 17:42:05: 21000000 INFO @ Sun, 21 Jun 2020 17:42:11: 22000000 INFO @ Sun, 21 Jun 2020 17:42:12: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:42:12: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:42:12: #1 total tags in treatment: 22297326 INFO @ Sun, 21 Jun 2020 17:42:12: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:42:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:42:13: #1 tags after filtering in treatment: 22297326 INFO @ Sun, 21 Jun 2020 17:42:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:42:13: #1 finished! INFO @ Sun, 21 Jun 2020 17:42:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:42:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:42:14: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 17:42:14: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:42:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113319/SRX113319.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。