Job ID = 6453386 SRX = SRX113314 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:11:03 prefetch.2.10.7: 1) Downloading 'SRR392924'... 2020-06-21T08:11:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:15:02 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:15:02 prefetch.2.10.7: 1) 'SRR392924' was downloaded successfully Read 21723799 spots for SRR392924/SRR392924.sra Written 21723799 spots for SRR392924/SRR392924.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:37 21723799 reads; of these: 21723799 (100.00%) were unpaired; of these: 132614 (0.61%) aligned 0 times 19044946 (87.67%) aligned exactly 1 time 2546239 (11.72%) aligned >1 times 99.39% overall alignment rate Time searching: 00:04:37 Overall time: 00:04:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4368294 / 21591185 = 0.2023 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:26:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:26:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:26:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:26:22: 1000000 INFO @ Sun, 21 Jun 2020 17:26:28: 2000000 INFO @ Sun, 21 Jun 2020 17:26:34: 3000000 INFO @ Sun, 21 Jun 2020 17:26:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:26:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:26:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:26:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:26:46: 5000000 INFO @ Sun, 21 Jun 2020 17:26:53: 6000000 INFO @ Sun, 21 Jun 2020 17:26:53: 1000000 INFO @ Sun, 21 Jun 2020 17:27:00: 7000000 INFO @ Sun, 21 Jun 2020 17:27:01: 2000000 INFO @ Sun, 21 Jun 2020 17:27:07: 8000000 INFO @ Sun, 21 Jun 2020 17:27:09: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:27:14: 9000000 INFO @ Sun, 21 Jun 2020 17:27:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:27:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:27:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:27:17: 4000000 INFO @ Sun, 21 Jun 2020 17:27:21: 10000000 INFO @ Sun, 21 Jun 2020 17:27:23: 1000000 INFO @ Sun, 21 Jun 2020 17:27:25: 5000000 INFO @ Sun, 21 Jun 2020 17:27:28: 11000000 INFO @ Sun, 21 Jun 2020 17:27:30: 2000000 INFO @ Sun, 21 Jun 2020 17:27:33: 6000000 INFO @ Sun, 21 Jun 2020 17:27:35: 12000000 INFO @ Sun, 21 Jun 2020 17:27:37: 3000000 INFO @ Sun, 21 Jun 2020 17:27:41: 7000000 INFO @ Sun, 21 Jun 2020 17:27:42: 13000000 INFO @ Sun, 21 Jun 2020 17:27:45: 4000000 INFO @ Sun, 21 Jun 2020 17:27:49: 8000000 INFO @ Sun, 21 Jun 2020 17:27:50: 14000000 INFO @ Sun, 21 Jun 2020 17:27:52: 5000000 INFO @ Sun, 21 Jun 2020 17:27:57: 15000000 INFO @ Sun, 21 Jun 2020 17:27:57: 9000000 INFO @ Sun, 21 Jun 2020 17:27:59: 6000000 INFO @ Sun, 21 Jun 2020 17:28:04: 16000000 INFO @ Sun, 21 Jun 2020 17:28:05: 10000000 INFO @ Sun, 21 Jun 2020 17:28:06: 7000000 INFO @ Sun, 21 Jun 2020 17:28:12: 17000000 INFO @ Sun, 21 Jun 2020 17:28:13: 8000000 INFO @ Sun, 21 Jun 2020 17:28:14: 11000000 INFO @ Sun, 21 Jun 2020 17:28:14: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:28:14: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:28:14: #1 total tags in treatment: 17222891 INFO @ Sun, 21 Jun 2020 17:28:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:28:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:28:14: #1 tags after filtering in treatment: 17222880 INFO @ Sun, 21 Jun 2020 17:28:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:28:14: #1 finished! INFO @ Sun, 21 Jun 2020 17:28:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:28:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:28:15: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 17:28:15: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:28:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:28:21: 9000000 INFO @ Sun, 21 Jun 2020 17:28:22: 12000000 INFO @ Sun, 21 Jun 2020 17:28:28: 10000000 INFO @ Sun, 21 Jun 2020 17:28:30: 13000000 INFO @ Sun, 21 Jun 2020 17:28:35: 11000000 INFO @ Sun, 21 Jun 2020 17:28:38: 14000000 INFO @ Sun, 21 Jun 2020 17:28:42: 12000000 INFO @ Sun, 21 Jun 2020 17:28:46: 15000000 INFO @ Sun, 21 Jun 2020 17:28:49: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:28:54: 16000000 INFO @ Sun, 21 Jun 2020 17:28:57: 14000000 INFO @ Sun, 21 Jun 2020 17:29:03: 17000000 INFO @ Sun, 21 Jun 2020 17:29:04: 15000000 INFO @ Sun, 21 Jun 2020 17:29:05: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:29:05: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:29:05: #1 total tags in treatment: 17222891 INFO @ Sun, 21 Jun 2020 17:29:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:29:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:29:05: #1 tags after filtering in treatment: 17222880 INFO @ Sun, 21 Jun 2020 17:29:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:29:05: #1 finished! INFO @ Sun, 21 Jun 2020 17:29:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:29:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:29:06: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 17:29:06: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:29:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:29:11: 16000000 INFO @ Sun, 21 Jun 2020 17:29:17: 17000000 INFO @ Sun, 21 Jun 2020 17:29:18: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:29:18: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:29:18: #1 total tags in treatment: 17222891 INFO @ Sun, 21 Jun 2020 17:29:18: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:29:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:29:19: #1 tags after filtering in treatment: 17222880 INFO @ Sun, 21 Jun 2020 17:29:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:29:19: #1 finished! INFO @ Sun, 21 Jun 2020 17:29:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:29:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:29:20: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 17:29:20: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:29:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113314/SRX113314.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。