Job ID = 6453382 SRX = SRX113308 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:00:34 prefetch.2.10.7: 1) Downloading 'SRR392915'... 2020-06-21T09:00:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:02:40 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:02:40 prefetch.2.10.7: 1) 'SRR392915' was downloaded successfully Read 21802283 spots for SRR392915/SRR392915.sra Written 21802283 spots for SRR392915/SRR392915.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:04 21802283 reads; of these: 21802283 (100.00%) were unpaired; of these: 597228 (2.74%) aligned 0 times 17456776 (80.07%) aligned exactly 1 time 3748279 (17.19%) aligned >1 times 97.26% overall alignment rate Time searching: 00:05:04 Overall time: 00:05:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4815596 / 21205055 = 0.2271 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:15:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:15:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:15:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:15:20: 1000000 INFO @ Sun, 21 Jun 2020 18:15:25: 2000000 INFO @ Sun, 21 Jun 2020 18:15:31: 3000000 INFO @ Sun, 21 Jun 2020 18:15:37: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:15:43: 5000000 INFO @ Sun, 21 Jun 2020 18:15:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:15:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:15:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:15:50: 6000000 INFO @ Sun, 21 Jun 2020 18:15:52: 1000000 INFO @ Sun, 21 Jun 2020 18:15:56: 7000000 INFO @ Sun, 21 Jun 2020 18:15:59: 2000000 INFO @ Sun, 21 Jun 2020 18:16:03: 8000000 INFO @ Sun, 21 Jun 2020 18:16:06: 3000000 INFO @ Sun, 21 Jun 2020 18:16:10: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:16:13: 4000000 INFO @ Sun, 21 Jun 2020 18:16:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:16:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:16:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:16:17: 10000000 INFO @ Sun, 21 Jun 2020 18:16:20: 5000000 INFO @ Sun, 21 Jun 2020 18:16:22: 1000000 INFO @ Sun, 21 Jun 2020 18:16:25: 11000000 INFO @ Sun, 21 Jun 2020 18:16:27: 6000000 INFO @ Sun, 21 Jun 2020 18:16:29: 2000000 INFO @ Sun, 21 Jun 2020 18:16:32: 12000000 INFO @ Sun, 21 Jun 2020 18:16:34: 7000000 INFO @ Sun, 21 Jun 2020 18:16:36: 3000000 INFO @ Sun, 21 Jun 2020 18:16:39: 13000000 INFO @ Sun, 21 Jun 2020 18:16:41: 8000000 INFO @ Sun, 21 Jun 2020 18:16:43: 4000000 INFO @ Sun, 21 Jun 2020 18:16:47: 14000000 INFO @ Sun, 21 Jun 2020 18:16:49: 9000000 INFO @ Sun, 21 Jun 2020 18:16:51: 5000000 INFO @ Sun, 21 Jun 2020 18:16:55: 15000000 INFO @ Sun, 21 Jun 2020 18:16:56: 10000000 INFO @ Sun, 21 Jun 2020 18:16:58: 6000000 INFO @ Sun, 21 Jun 2020 18:17:02: 16000000 INFO @ Sun, 21 Jun 2020 18:17:03: 11000000 INFO @ Sun, 21 Jun 2020 18:17:05: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 18:17:05: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 18:17:05: #1 total tags in treatment: 16389459 INFO @ Sun, 21 Jun 2020 18:17:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:17:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:17:05: 7000000 INFO @ Sun, 21 Jun 2020 18:17:06: #1 tags after filtering in treatment: 16389458 INFO @ Sun, 21 Jun 2020 18:17:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:17:06: #1 finished! INFO @ Sun, 21 Jun 2020 18:17:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:17:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:17:07: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 18:17:07: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:17:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:17:10: 12000000 INFO @ Sun, 21 Jun 2020 18:17:13: 8000000 INFO @ Sun, 21 Jun 2020 18:17:18: 13000000 INFO @ Sun, 21 Jun 2020 18:17:20: 9000000 INFO @ Sun, 21 Jun 2020 18:17:26: 14000000 INFO @ Sun, 21 Jun 2020 18:17:27: 10000000 INFO @ Sun, 21 Jun 2020 18:17:33: 15000000 INFO @ Sun, 21 Jun 2020 18:17:35: 11000000 INFO @ Sun, 21 Jun 2020 18:17:41: 16000000 INFO @ Sun, 21 Jun 2020 18:17:42: 12000000 INFO @ Sun, 21 Jun 2020 18:17:44: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 18:17:44: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 18:17:44: #1 total tags in treatment: 16389459 INFO @ Sun, 21 Jun 2020 18:17:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:17:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:17:45: #1 tags after filtering in treatment: 16389458 INFO @ Sun, 21 Jun 2020 18:17:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:17:45: #1 finished! INFO @ Sun, 21 Jun 2020 18:17:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:17:45: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:17:46: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 18:17:46: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:17:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:17:50: 13000000 INFO @ Sun, 21 Jun 2020 18:17:58: 14000000 INFO @ Sun, 21 Jun 2020 18:18:05: 15000000 INFO @ Sun, 21 Jun 2020 18:18:13: 16000000 INFO @ Sun, 21 Jun 2020 18:18:16: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 18:18:16: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 18:18:16: #1 total tags in treatment: 16389459 INFO @ Sun, 21 Jun 2020 18:18:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:18:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:18:17: #1 tags after filtering in treatment: 16389458 INFO @ Sun, 21 Jun 2020 18:18:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:18:17: #1 finished! INFO @ Sun, 21 Jun 2020 18:18:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:18:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:18:18: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 18:18:18: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:18:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113308/SRX113308.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。