Job ID = 6453379 SRX = SRX113305 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:16:48 prefetch.2.10.7: 1) Downloading 'SRR392910'... 2020-06-21T08:16:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:19:52 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:19:52 prefetch.2.10.7: 1) 'SRR392910' was downloaded successfully Read 17982328 spots for SRR392910/SRR392910.sra Written 17982328 spots for SRR392910/SRR392910.sra 2020-06-21T08:20:54 prefetch.2.10.7: 1) Downloading 'SRR392911'... 2020-06-21T08:20:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:21:50 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:21:50 prefetch.2.10.7: 'SRR392911' is valid 2020-06-21T08:21:50 prefetch.2.10.7: 1) 'SRR392911' was downloaded successfully Read 5177634 spots for SRR392911/SRR392911.sra Written 5177634 spots for SRR392911/SRR392911.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:35 23159962 reads; of these: 23159962 (100.00%) were unpaired; of these: 1228458 (5.30%) aligned 0 times 18392489 (79.42%) aligned exactly 1 time 3539015 (15.28%) aligned >1 times 94.70% overall alignment rate Time searching: 00:04:35 Overall time: 00:04:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5040572 / 21931504 = 0.2298 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:31:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:31:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:31:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:32:05: 1000000 INFO @ Sun, 21 Jun 2020 17:32:12: 2000000 INFO @ Sun, 21 Jun 2020 17:32:19: 3000000 INFO @ Sun, 21 Jun 2020 17:32:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:32:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:32:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:32:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:32:33: 5000000 INFO @ Sun, 21 Jun 2020 17:32:36: 1000000 INFO @ Sun, 21 Jun 2020 17:32:40: 6000000 INFO @ Sun, 21 Jun 2020 17:32:43: 2000000 INFO @ Sun, 21 Jun 2020 17:32:47: 7000000 INFO @ Sun, 21 Jun 2020 17:32:50: 3000000 INFO @ Sun, 21 Jun 2020 17:32:54: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:32:56: 4000000 INFO @ Sun, 21 Jun 2020 17:32:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:32:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:32:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:33:01: 9000000 INFO @ Sun, 21 Jun 2020 17:33:03: 5000000 INFO @ Sun, 21 Jun 2020 17:33:05: 1000000 INFO @ Sun, 21 Jun 2020 17:33:08: 10000000 INFO @ Sun, 21 Jun 2020 17:33:10: 6000000 INFO @ Sun, 21 Jun 2020 17:33:12: 2000000 INFO @ Sun, 21 Jun 2020 17:33:16: 11000000 INFO @ Sun, 21 Jun 2020 17:33:17: 7000000 INFO @ Sun, 21 Jun 2020 17:33:19: 3000000 INFO @ Sun, 21 Jun 2020 17:33:23: 12000000 INFO @ Sun, 21 Jun 2020 17:33:24: 8000000 INFO @ Sun, 21 Jun 2020 17:33:26: 4000000 INFO @ Sun, 21 Jun 2020 17:33:30: 13000000 INFO @ Sun, 21 Jun 2020 17:33:31: 9000000 INFO @ Sun, 21 Jun 2020 17:33:33: 5000000 INFO @ Sun, 21 Jun 2020 17:33:38: 14000000 INFO @ Sun, 21 Jun 2020 17:33:38: 10000000 INFO @ Sun, 21 Jun 2020 17:33:40: 6000000 INFO @ Sun, 21 Jun 2020 17:33:45: 11000000 INFO @ Sun, 21 Jun 2020 17:33:45: 15000000 INFO @ Sun, 21 Jun 2020 17:33:47: 7000000 INFO @ Sun, 21 Jun 2020 17:33:52: 12000000 INFO @ Sun, 21 Jun 2020 17:33:52: 16000000 INFO @ Sun, 21 Jun 2020 17:33:54: 8000000 INFO @ Sun, 21 Jun 2020 17:33:59: 13000000 INFO @ Sun, 21 Jun 2020 17:33:59: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:33:59: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:33:59: #1 total tags in treatment: 16890932 INFO @ Sun, 21 Jun 2020 17:33:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:33:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:34:00: #1 tags after filtering in treatment: 16890932 INFO @ Sun, 21 Jun 2020 17:34:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:34:00: #1 finished! INFO @ Sun, 21 Jun 2020 17:34:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:34:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:34:01: #2 number of paired peaks: 46 WARNING @ Sun, 21 Jun 2020 17:34:01: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:34:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:34:01: 9000000 INFO @ Sun, 21 Jun 2020 17:34:06: 14000000 INFO @ Sun, 21 Jun 2020 17:34:08: 10000000 INFO @ Sun, 21 Jun 2020 17:34:12: 15000000 INFO @ Sun, 21 Jun 2020 17:34:14: 11000000 INFO @ Sun, 21 Jun 2020 17:34:18: 16000000 INFO @ Sun, 21 Jun 2020 17:34:21: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:34:25: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:34:25: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:34:25: #1 total tags in treatment: 16890932 INFO @ Sun, 21 Jun 2020 17:34:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:34:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:34:25: #1 tags after filtering in treatment: 16890932 INFO @ Sun, 21 Jun 2020 17:34:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:34:25: #1 finished! INFO @ Sun, 21 Jun 2020 17:34:25: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:34:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:34:26: #2 number of paired peaks: 46 WARNING @ Sun, 21 Jun 2020 17:34:26: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:34:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:34:27: 13000000 INFO @ Sun, 21 Jun 2020 17:34:32: 14000000 INFO @ Sun, 21 Jun 2020 17:34:38: 15000000 INFO @ Sun, 21 Jun 2020 17:34:43: 16000000 INFO @ Sun, 21 Jun 2020 17:34:48: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:34:48: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:34:48: #1 total tags in treatment: 16890932 INFO @ Sun, 21 Jun 2020 17:34:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:34:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:34:49: #1 tags after filtering in treatment: 16890932 INFO @ Sun, 21 Jun 2020 17:34:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:34:49: #1 finished! INFO @ Sun, 21 Jun 2020 17:34:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:34:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:34:50: #2 number of paired peaks: 46 WARNING @ Sun, 21 Jun 2020 17:34:50: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:34:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113305/SRX113305.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。