Job ID = 6453378 SRX = SRX113304 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:24:32 prefetch.2.10.7: 1) Downloading 'SRR392909'... 2020-06-21T08:24:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:28:09 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:28:09 prefetch.2.10.7: 1) 'SRR392909' was downloaded successfully Read 19875673 spots for SRR392909/SRR392909.sra Written 19875673 spots for SRR392909/SRR392909.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:10 19875673 reads; of these: 19875673 (100.00%) were unpaired; of these: 861742 (4.34%) aligned 0 times 16274865 (81.88%) aligned exactly 1 time 2739066 (13.78%) aligned >1 times 95.66% overall alignment rate Time searching: 00:05:10 Overall time: 00:05:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3602058 / 19013931 = 0.1894 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:40:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:40:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:40:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:40:53: 1000000 INFO @ Sun, 21 Jun 2020 17:40:59: 2000000 INFO @ Sun, 21 Jun 2020 17:41:05: 3000000 INFO @ Sun, 21 Jun 2020 17:41:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:41:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:41:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:41:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:41:17: 5000000 INFO @ Sun, 21 Jun 2020 17:41:24: 1000000 INFO @ Sun, 21 Jun 2020 17:41:24: 6000000 INFO @ Sun, 21 Jun 2020 17:41:31: 7000000 INFO @ Sun, 21 Jun 2020 17:41:31: 2000000 INFO @ Sun, 21 Jun 2020 17:41:37: 8000000 INFO @ Sun, 21 Jun 2020 17:41:37: 3000000 INFO @ Sun, 21 Jun 2020 17:41:44: 9000000 INFO @ Sun, 21 Jun 2020 17:41:44: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:41:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:41:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:41:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:41:51: 10000000 INFO @ Sun, 21 Jun 2020 17:41:51: 5000000 INFO @ Sun, 21 Jun 2020 17:41:54: 1000000 INFO @ Sun, 21 Jun 2020 17:41:58: 11000000 INFO @ Sun, 21 Jun 2020 17:41:59: 6000000 INFO @ Sun, 21 Jun 2020 17:42:02: 2000000 INFO @ Sun, 21 Jun 2020 17:42:06: 12000000 INFO @ Sun, 21 Jun 2020 17:42:06: 7000000 INFO @ Sun, 21 Jun 2020 17:42:09: 3000000 INFO @ Sun, 21 Jun 2020 17:42:13: 8000000 INFO @ Sun, 21 Jun 2020 17:42:14: 13000000 INFO @ Sun, 21 Jun 2020 17:42:16: 4000000 INFO @ Sun, 21 Jun 2020 17:42:20: 9000000 INFO @ Sun, 21 Jun 2020 17:42:21: 14000000 INFO @ Sun, 21 Jun 2020 17:42:24: 5000000 INFO @ Sun, 21 Jun 2020 17:42:28: 10000000 INFO @ Sun, 21 Jun 2020 17:42:29: 15000000 INFO @ Sun, 21 Jun 2020 17:42:31: 6000000 INFO @ Sun, 21 Jun 2020 17:42:32: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:42:32: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:42:32: #1 total tags in treatment: 15411873 INFO @ Sun, 21 Jun 2020 17:42:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:42:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:42:33: #1 tags after filtering in treatment: 15411857 INFO @ Sun, 21 Jun 2020 17:42:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:42:33: #1 finished! INFO @ Sun, 21 Jun 2020 17:42:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:42:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:42:34: #2 number of paired peaks: 35 WARNING @ Sun, 21 Jun 2020 17:42:34: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:42:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:42:35: 11000000 INFO @ Sun, 21 Jun 2020 17:42:38: 7000000 INFO @ Sun, 21 Jun 2020 17:42:42: 12000000 INFO @ Sun, 21 Jun 2020 17:42:45: 8000000 INFO @ Sun, 21 Jun 2020 17:42:49: 13000000 INFO @ Sun, 21 Jun 2020 17:42:52: 9000000 INFO @ Sun, 21 Jun 2020 17:42:56: 14000000 INFO @ Sun, 21 Jun 2020 17:42:59: 10000000 INFO @ Sun, 21 Jun 2020 17:43:03: 15000000 INFO @ Sun, 21 Jun 2020 17:43:05: 11000000 INFO @ Sun, 21 Jun 2020 17:43:06: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:43:06: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:43:06: #1 total tags in treatment: 15411873 INFO @ Sun, 21 Jun 2020 17:43:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:43:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:43:07: #1 tags after filtering in treatment: 15411857 INFO @ Sun, 21 Jun 2020 17:43:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:43:07: #1 finished! INFO @ Sun, 21 Jun 2020 17:43:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:43:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:43:08: #2 number of paired peaks: 35 WARNING @ Sun, 21 Jun 2020 17:43:08: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:43:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:43:12: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:43:20: 13000000 INFO @ Sun, 21 Jun 2020 17:43:27: 14000000 INFO @ Sun, 21 Jun 2020 17:43:34: 15000000 INFO @ Sun, 21 Jun 2020 17:43:38: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:43:38: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:43:38: #1 total tags in treatment: 15411873 INFO @ Sun, 21 Jun 2020 17:43:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:43:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:43:39: #1 tags after filtering in treatment: 15411857 INFO @ Sun, 21 Jun 2020 17:43:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:43:39: #1 finished! INFO @ Sun, 21 Jun 2020 17:43:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:43:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:43:40: #2 number of paired peaks: 35 WARNING @ Sun, 21 Jun 2020 17:43:40: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:43:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113304/SRX113304.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。