Job ID = 6453377 SRX = SRX113303 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:26:56 prefetch.2.10.7: 1) Downloading 'SRR392907'... 2020-06-21T08:26:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:27:52 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:27:52 prefetch.2.10.7: 'SRR392907' is valid 2020-06-21T08:27:52 prefetch.2.10.7: 1) 'SRR392907' was downloaded successfully Read 2188064 spots for SRR392907/SRR392907.sra Written 2188064 spots for SRR392907/SRR392907.sra 2020-06-21T08:28:09 prefetch.2.10.7: 1) Downloading 'SRR392908'... 2020-06-21T08:28:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:31:38 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:31:38 prefetch.2.10.7: 1) 'SRR392908' was downloaded successfully Read 20889019 spots for SRR392908/SRR392908.sra Written 20889019 spots for SRR392908/SRR392908.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:10 23077083 reads; of these: 23077083 (100.00%) were unpaired; of these: 3286117 (14.24%) aligned 0 times 16446222 (71.27%) aligned exactly 1 time 3344744 (14.49%) aligned >1 times 85.76% overall alignment rate Time searching: 00:05:10 Overall time: 00:05:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4777729 / 19790966 = 0.2414 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:42:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:42:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:42:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:43:05: 1000000 INFO @ Sun, 21 Jun 2020 17:43:10: 2000000 INFO @ Sun, 21 Jun 2020 17:43:15: 3000000 INFO @ Sun, 21 Jun 2020 17:43:20: 4000000 INFO @ Sun, 21 Jun 2020 17:43:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:43:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:43:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:43:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:43:31: 6000000 INFO @ Sun, 21 Jun 2020 17:43:35: 1000000 INFO @ Sun, 21 Jun 2020 17:43:36: 7000000 INFO @ Sun, 21 Jun 2020 17:43:40: 2000000 INFO @ Sun, 21 Jun 2020 17:43:42: 8000000 INFO @ Sun, 21 Jun 2020 17:43:45: 3000000 INFO @ Sun, 21 Jun 2020 17:43:47: 9000000 INFO @ Sun, 21 Jun 2020 17:43:51: 4000000 INFO @ Sun, 21 Jun 2020 17:43:52: 10000000 INFO @ Sun, 21 Jun 2020 17:43:56: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:43:58: 11000000 INFO @ Sun, 21 Jun 2020 17:43:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:43:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:43:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:44:01: 6000000 INFO @ Sun, 21 Jun 2020 17:44:03: 12000000 INFO @ Sun, 21 Jun 2020 17:44:05: 1000000 INFO @ Sun, 21 Jun 2020 17:44:07: 7000000 INFO @ Sun, 21 Jun 2020 17:44:08: 13000000 INFO @ Sun, 21 Jun 2020 17:44:10: 2000000 INFO @ Sun, 21 Jun 2020 17:44:12: 8000000 INFO @ Sun, 21 Jun 2020 17:44:14: 14000000 INFO @ Sun, 21 Jun 2020 17:44:16: 3000000 INFO @ Sun, 21 Jun 2020 17:44:17: 9000000 INFO @ Sun, 21 Jun 2020 17:44:19: 15000000 INFO @ Sun, 21 Jun 2020 17:44:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:44:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:44:19: #1 total tags in treatment: 15013237 INFO @ Sun, 21 Jun 2020 17:44:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:44:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:44:20: #1 tags after filtering in treatment: 15013237 INFO @ Sun, 21 Jun 2020 17:44:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:44:20: #1 finished! INFO @ Sun, 21 Jun 2020 17:44:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:44:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:44:21: 4000000 INFO @ Sun, 21 Jun 2020 17:44:21: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 17:44:21: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:44:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:44:22: 10000000 INFO @ Sun, 21 Jun 2020 17:44:26: 5000000 INFO @ Sun, 21 Jun 2020 17:44:28: 11000000 INFO @ Sun, 21 Jun 2020 17:44:32: 6000000 INFO @ Sun, 21 Jun 2020 17:44:33: 12000000 INFO @ Sun, 21 Jun 2020 17:44:37: 7000000 INFO @ Sun, 21 Jun 2020 17:44:39: 13000000 INFO @ Sun, 21 Jun 2020 17:44:42: 8000000 INFO @ Sun, 21 Jun 2020 17:44:44: 14000000 INFO @ Sun, 21 Jun 2020 17:44:48: 9000000 INFO @ Sun, 21 Jun 2020 17:44:49: 15000000 INFO @ Sun, 21 Jun 2020 17:44:50: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:44:50: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:44:50: #1 total tags in treatment: 15013237 INFO @ Sun, 21 Jun 2020 17:44:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:44:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:44:50: #1 tags after filtering in treatment: 15013237 INFO @ Sun, 21 Jun 2020 17:44:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:44:50: #1 finished! INFO @ Sun, 21 Jun 2020 17:44:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:44:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:44:51: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 17:44:51: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:44:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:44:53: 10000000 INFO @ Sun, 21 Jun 2020 17:44:58: 11000000 INFO @ Sun, 21 Jun 2020 17:45:04: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:45:09: 13000000 INFO @ Sun, 21 Jun 2020 17:45:14: 14000000 INFO @ Sun, 21 Jun 2020 17:45:20: 15000000 INFO @ Sun, 21 Jun 2020 17:45:20: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 17:45:20: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 17:45:20: #1 total tags in treatment: 15013237 INFO @ Sun, 21 Jun 2020 17:45:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:45:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:45:21: #1 tags after filtering in treatment: 15013237 INFO @ Sun, 21 Jun 2020 17:45:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:45:21: #1 finished! INFO @ Sun, 21 Jun 2020 17:45:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:45:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:45:22: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 17:45:22: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:45:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX113303/SRX113303.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。