Job ID = 6453352 SRX = SRX111821 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:23:17 prefetch.2.10.7: 1) Downloading 'SRR390288'... 2020-06-21T08:23:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:28:01 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:28:01 prefetch.2.10.7: 'SRR390288' is valid 2020-06-21T08:28:01 prefetch.2.10.7: 1) 'SRR390288' was downloaded successfully Read 20417854 spots for SRR390288/SRR390288.sra Written 20417854 spots for SRR390288/SRR390288.sra 2020-06-21T08:29:07 prefetch.2.10.7: 1) Downloading 'SRR390289'... 2020-06-21T08:29:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:31:55 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:31:56 prefetch.2.10.7: 'SRR390289' is valid 2020-06-21T08:31:56 prefetch.2.10.7: 1) 'SRR390289' was downloaded successfully Read 16684498 spots for SRR390289/SRR390289.sra Written 16684498 spots for SRR390289/SRR390289.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:25 37102352 reads; of these: 37102352 (100.00%) were unpaired; of these: 15304690 (41.25%) aligned 0 times 18614051 (50.17%) aligned exactly 1 time 3183611 (8.58%) aligned >1 times 58.75% overall alignment rate Time searching: 00:05:25 Overall time: 00:05:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5139296 / 21797662 = 0.2358 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:43:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:43:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:43:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:43:26: 1000000 INFO @ Sun, 21 Jun 2020 17:43:31: 2000000 INFO @ Sun, 21 Jun 2020 17:43:36: 3000000 INFO @ Sun, 21 Jun 2020 17:43:41: 4000000 INFO @ Sun, 21 Jun 2020 17:43:46: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:43:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:43:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:43:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:43:51: 6000000 INFO @ Sun, 21 Jun 2020 17:43:55: 1000000 INFO @ Sun, 21 Jun 2020 17:43:56: 7000000 INFO @ Sun, 21 Jun 2020 17:44:01: 2000000 INFO @ Sun, 21 Jun 2020 17:44:01: 8000000 INFO @ Sun, 21 Jun 2020 17:44:06: 3000000 INFO @ Sun, 21 Jun 2020 17:44:06: 9000000 INFO @ Sun, 21 Jun 2020 17:44:11: 4000000 INFO @ Sun, 21 Jun 2020 17:44:12: 10000000 INFO @ Sun, 21 Jun 2020 17:44:16: 5000000 INFO @ Sun, 21 Jun 2020 17:44:17: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:44:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:44:20: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:44:20: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:44:21: 6000000 INFO @ Sun, 21 Jun 2020 17:44:22: 12000000 INFO @ Sun, 21 Jun 2020 17:44:25: 1000000 INFO @ Sun, 21 Jun 2020 17:44:27: 7000000 INFO @ Sun, 21 Jun 2020 17:44:27: 13000000 INFO @ Sun, 21 Jun 2020 17:44:31: 2000000 INFO @ Sun, 21 Jun 2020 17:44:32: 8000000 INFO @ Sun, 21 Jun 2020 17:44:33: 14000000 INFO @ Sun, 21 Jun 2020 17:44:36: 3000000 INFO @ Sun, 21 Jun 2020 17:44:37: 9000000 INFO @ Sun, 21 Jun 2020 17:44:38: 15000000 INFO @ Sun, 21 Jun 2020 17:44:41: 4000000 INFO @ Sun, 21 Jun 2020 17:44:43: 10000000 INFO @ Sun, 21 Jun 2020 17:44:44: 16000000 INFO @ Sun, 21 Jun 2020 17:44:46: 5000000 INFO @ Sun, 21 Jun 2020 17:44:47: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:44:47: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:44:47: #1 total tags in treatment: 16658366 INFO @ Sun, 21 Jun 2020 17:44:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:44:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:44:48: #1 tags after filtering in treatment: 16658361 INFO @ Sun, 21 Jun 2020 17:44:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:44:48: #1 finished! INFO @ Sun, 21 Jun 2020 17:44:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:44:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:44:48: 11000000 INFO @ Sun, 21 Jun 2020 17:44:49: #2 number of paired peaks: 42 WARNING @ Sun, 21 Jun 2020 17:44:49: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:44:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:44:52: 6000000 INFO @ Sun, 21 Jun 2020 17:44:53: 12000000 INFO @ Sun, 21 Jun 2020 17:44:57: 7000000 INFO @ Sun, 21 Jun 2020 17:44:58: 13000000 INFO @ Sun, 21 Jun 2020 17:45:02: 8000000 INFO @ Sun, 21 Jun 2020 17:45:04: 14000000 INFO @ Sun, 21 Jun 2020 17:45:07: 9000000 INFO @ Sun, 21 Jun 2020 17:45:09: 15000000 INFO @ Sun, 21 Jun 2020 17:45:12: 10000000 INFO @ Sun, 21 Jun 2020 17:45:14: 16000000 INFO @ Sun, 21 Jun 2020 17:45:18: 11000000 INFO @ Sun, 21 Jun 2020 17:45:18: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:45:18: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:45:18: #1 total tags in treatment: 16658366 INFO @ Sun, 21 Jun 2020 17:45:18: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:45:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:45:18: #1 tags after filtering in treatment: 16658361 INFO @ Sun, 21 Jun 2020 17:45:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:45:18: #1 finished! INFO @ Sun, 21 Jun 2020 17:45:18: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:45:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:45:19: #2 number of paired peaks: 42 WARNING @ Sun, 21 Jun 2020 17:45:19: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:45:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:45:23: 12000000 INFO @ Sun, 21 Jun 2020 17:45:28: 13000000 INFO @ Sun, 21 Jun 2020 17:45:33: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:45:38: 15000000 INFO @ Sun, 21 Jun 2020 17:45:43: 16000000 INFO @ Sun, 21 Jun 2020 17:45:46: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:45:46: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:45:46: #1 total tags in treatment: 16658366 INFO @ Sun, 21 Jun 2020 17:45:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:45:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:45:47: #1 tags after filtering in treatment: 16658361 INFO @ Sun, 21 Jun 2020 17:45:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:45:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:45:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:45:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:45:48: #2 number of paired peaks: 42 WARNING @ Sun, 21 Jun 2020 17:45:48: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:45:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111821/SRX111821.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。