Job ID = 6453351 SRX = SRX111820 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:26:56 prefetch.2.10.7: 1) Downloading 'SRR390287'... 2020-06-21T08:26:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:30:16 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:30:16 prefetch.2.10.7: 1) 'SRR390287' was downloaded successfully Read 25111745 spots for SRR390287/SRR390287.sra Written 25111745 spots for SRR390287/SRR390287.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:08 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 485129 (1.93%) aligned 0 times 20893369 (83.20%) aligned exactly 1 time 3733247 (14.87%) aligned >1 times 98.07% overall alignment rate Time searching: 00:05:08 Overall time: 00:05:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6038164 / 24626616 = 0.2452 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:42:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:42:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:42:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:42:07: 1000000 INFO @ Sun, 21 Jun 2020 17:42:13: 2000000 INFO @ Sun, 21 Jun 2020 17:42:19: 3000000 INFO @ Sun, 21 Jun 2020 17:42:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:42:30: 5000000 INFO @ Sun, 21 Jun 2020 17:42:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:42:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:42:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:42:36: 1000000 INFO @ Sun, 21 Jun 2020 17:42:37: 6000000 INFO @ Sun, 21 Jun 2020 17:42:42: 2000000 INFO @ Sun, 21 Jun 2020 17:42:43: 7000000 INFO @ Sun, 21 Jun 2020 17:42:48: 3000000 INFO @ Sun, 21 Jun 2020 17:42:50: 8000000 INFO @ Sun, 21 Jun 2020 17:42:54: 4000000 INFO @ Sun, 21 Jun 2020 17:42:56: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:43:00: 5000000 INFO @ Sun, 21 Jun 2020 17:43:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:43:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:43:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:43:03: 10000000 INFO @ Sun, 21 Jun 2020 17:43:05: 6000000 INFO @ Sun, 21 Jun 2020 17:43:07: 1000000 INFO @ Sun, 21 Jun 2020 17:43:09: 11000000 INFO @ Sun, 21 Jun 2020 17:43:11: 7000000 INFO @ Sun, 21 Jun 2020 17:43:12: 2000000 INFO @ Sun, 21 Jun 2020 17:43:16: 12000000 INFO @ Sun, 21 Jun 2020 17:43:17: 8000000 INFO @ Sun, 21 Jun 2020 17:43:18: 3000000 INFO @ Sun, 21 Jun 2020 17:43:22: 13000000 INFO @ Sun, 21 Jun 2020 17:43:23: 9000000 INFO @ Sun, 21 Jun 2020 17:43:24: 4000000 INFO @ Sun, 21 Jun 2020 17:43:29: 10000000 INFO @ Sun, 21 Jun 2020 17:43:29: 14000000 INFO @ Sun, 21 Jun 2020 17:43:30: 5000000 INFO @ Sun, 21 Jun 2020 17:43:34: 11000000 INFO @ Sun, 21 Jun 2020 17:43:35: 15000000 INFO @ Sun, 21 Jun 2020 17:43:36: 6000000 INFO @ Sun, 21 Jun 2020 17:43:40: 12000000 INFO @ Sun, 21 Jun 2020 17:43:41: 7000000 INFO @ Sun, 21 Jun 2020 17:43:42: 16000000 INFO @ Sun, 21 Jun 2020 17:43:46: 13000000 INFO @ Sun, 21 Jun 2020 17:43:47: 8000000 INFO @ Sun, 21 Jun 2020 17:43:49: 17000000 INFO @ Sun, 21 Jun 2020 17:43:52: 14000000 INFO @ Sun, 21 Jun 2020 17:43:53: 9000000 INFO @ Sun, 21 Jun 2020 17:43:56: 18000000 INFO @ Sun, 21 Jun 2020 17:43:58: 15000000 INFO @ Sun, 21 Jun 2020 17:43:59: 10000000 INFO @ Sun, 21 Jun 2020 17:44:00: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:44:00: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:44:00: #1 total tags in treatment: 18588452 INFO @ Sun, 21 Jun 2020 17:44:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:44:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:44:01: #1 tags after filtering in treatment: 18588448 INFO @ Sun, 21 Jun 2020 17:44:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:44:01: #1 finished! INFO @ Sun, 21 Jun 2020 17:44:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:44:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:44:02: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 17:44:02: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:44:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:44:04: 16000000 INFO @ Sun, 21 Jun 2020 17:44:04: 11000000 INFO @ Sun, 21 Jun 2020 17:44:10: 12000000 INFO @ Sun, 21 Jun 2020 17:44:10: 17000000 INFO @ Sun, 21 Jun 2020 17:44:16: 13000000 INFO @ Sun, 21 Jun 2020 17:44:16: 18000000 INFO @ Sun, 21 Jun 2020 17:44:20: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:44:20: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:44:20: #1 total tags in treatment: 18588452 INFO @ Sun, 21 Jun 2020 17:44:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:44:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:44:20: #1 tags after filtering in treatment: 18588448 INFO @ Sun, 21 Jun 2020 17:44:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:44:20: #1 finished! INFO @ Sun, 21 Jun 2020 17:44:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:44:20: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:44:21: 14000000 INFO @ Sun, 21 Jun 2020 17:44:21: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 17:44:21: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:44:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:44:27: 15000000 INFO @ Sun, 21 Jun 2020 17:44:32: 16000000 INFO @ Sun, 21 Jun 2020 17:44:38: 17000000 INFO @ Sun, 21 Jun 2020 17:44:43: 18000000 INFO @ Sun, 21 Jun 2020 17:44:46: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:44:46: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:44:46: #1 total tags in treatment: 18588452 INFO @ Sun, 21 Jun 2020 17:44:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:44:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:44:47: #1 tags after filtering in treatment: 18588448 INFO @ Sun, 21 Jun 2020 17:44:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:44:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:44:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:44:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:44:48: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 17:44:48: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:44:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111820/SRX111820.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。