Job ID = 6453350 SRX = SRX111819 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:13:03 prefetch.2.10.7: 1) Downloading 'SRR390285'... 2020-06-21T08:13:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:14:54 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:14:54 prefetch.2.10.7: 'SRR390285' is valid 2020-06-21T08:14:54 prefetch.2.10.7: 1) 'SRR390285' was downloaded successfully Read 15013137 spots for SRR390285/SRR390285.sra Written 15013137 spots for SRR390285/SRR390285.sra 2020-06-21T08:15:47 prefetch.2.10.7: 1) Downloading 'SRR390286'... 2020-06-21T08:15:47 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:16:41 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:16:41 prefetch.2.10.7: 'SRR390286' is valid 2020-06-21T08:16:41 prefetch.2.10.7: 1) 'SRR390286' was downloaded successfully Read 7215432 spots for SRR390286/SRR390286.sra Written 7215432 spots for SRR390286/SRR390286.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:41 22228569 reads; of these: 22228569 (100.00%) were unpaired; of these: 8832200 (39.73%) aligned 0 times 11672047 (52.51%) aligned exactly 1 time 1724322 (7.76%) aligned >1 times 60.27% overall alignment rate Time searching: 00:03:41 Overall time: 00:03:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1950722 / 13396369 = 0.1456 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:24:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:24:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:24:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:24:34: 1000000 INFO @ Sun, 21 Jun 2020 17:24:38: 2000000 INFO @ Sun, 21 Jun 2020 17:24:43: 3000000 INFO @ Sun, 21 Jun 2020 17:24:48: 4000000 INFO @ Sun, 21 Jun 2020 17:24:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:24:58: 6000000 INFO @ Sun, 21 Jun 2020 17:24:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:24:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:24:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:25:02: 7000000 INFO @ Sun, 21 Jun 2020 17:25:03: 1000000 INFO @ Sun, 21 Jun 2020 17:25:07: 8000000 INFO @ Sun, 21 Jun 2020 17:25:08: 2000000 INFO @ Sun, 21 Jun 2020 17:25:12: 9000000 INFO @ Sun, 21 Jun 2020 17:25:13: 3000000 INFO @ Sun, 21 Jun 2020 17:25:18: 10000000 INFO @ Sun, 21 Jun 2020 17:25:18: 4000000 INFO @ Sun, 21 Jun 2020 17:25:23: 11000000 INFO @ Sun, 21 Jun 2020 17:25:23: 5000000 INFO @ Sun, 21 Jun 2020 17:25:25: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:25:25: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:25:25: #1 total tags in treatment: 11445647 INFO @ Sun, 21 Jun 2020 17:25:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:25:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:25:26: #1 tags after filtering in treatment: 11445619 INFO @ Sun, 21 Jun 2020 17:25:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:25:26: #1 finished! INFO @ Sun, 21 Jun 2020 17:25:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:25:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:25:26: #2 number of paired peaks: 16 WARNING @ Sun, 21 Jun 2020 17:25:26: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:25:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.05_model.r’: No such file or directory BedGraph に変換中... rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:25:28: 6000000 INFO @ Sun, 21 Jun 2020 17:25:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:25:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:25:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:25:33: 7000000 INFO @ Sun, 21 Jun 2020 17:25:34: 1000000 INFO @ Sun, 21 Jun 2020 17:25:38: 8000000 INFO @ Sun, 21 Jun 2020 17:25:38: 2000000 INFO @ Sun, 21 Jun 2020 17:25:43: 9000000 INFO @ Sun, 21 Jun 2020 17:25:43: 3000000 INFO @ Sun, 21 Jun 2020 17:25:48: 10000000 INFO @ Sun, 21 Jun 2020 17:25:48: 4000000 INFO @ Sun, 21 Jun 2020 17:25:53: 11000000 INFO @ Sun, 21 Jun 2020 17:25:53: 5000000 INFO @ Sun, 21 Jun 2020 17:25:56: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:25:56: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:25:56: #1 total tags in treatment: 11445647 INFO @ Sun, 21 Jun 2020 17:25:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:25:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:25:56: #1 tags after filtering in treatment: 11445619 INFO @ Sun, 21 Jun 2020 17:25:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:25:56: #1 finished! INFO @ Sun, 21 Jun 2020 17:25:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:25:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:25:57: #2 number of paired peaks: 16 WARNING @ Sun, 21 Jun 2020 17:25:57: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:25:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:25:58: 6000000 INFO @ Sun, 21 Jun 2020 17:26:03: 7000000 INFO @ Sun, 21 Jun 2020 17:26:08: 8000000 INFO @ Sun, 21 Jun 2020 17:26:13: 9000000 INFO @ Sun, 21 Jun 2020 17:26:18: 10000000 INFO @ Sun, 21 Jun 2020 17:26:23: 11000000 INFO @ Sun, 21 Jun 2020 17:26:25: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:26:25: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:26:25: #1 total tags in treatment: 11445647 INFO @ Sun, 21 Jun 2020 17:26:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:26:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:26:26: #1 tags after filtering in treatment: 11445619 INFO @ Sun, 21 Jun 2020 17:26:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:26:26: #1 finished! INFO @ Sun, 21 Jun 2020 17:26:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:26:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:26:27: #2 number of paired peaks: 16 WARNING @ Sun, 21 Jun 2020 17:26:27: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:26:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111819/SRX111819.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。