Job ID = 6453349 SRX = SRX111818 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:15:33 prefetch.2.10.7: 1) Downloading 'SRR390284'... 2020-06-21T08:15:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:18:31 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:18:31 prefetch.2.10.7: 1) 'SRR390284' was downloaded successfully Read 23630901 spots for SRR390284/SRR390284.sra Written 23630901 spots for SRR390284/SRR390284.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:14 23630901 reads; of these: 23630901 (100.00%) were unpaired; of these: 634778 (2.69%) aligned 0 times 18561842 (78.55%) aligned exactly 1 time 4434281 (18.76%) aligned >1 times 97.31% overall alignment rate Time searching: 00:05:14 Overall time: 00:05:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5207384 / 22996123 = 0.2264 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:30:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:30:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:30:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:30:10: 1000000 INFO @ Sun, 21 Jun 2020 17:30:15: 2000000 INFO @ Sun, 21 Jun 2020 17:30:20: 3000000 INFO @ Sun, 21 Jun 2020 17:30:25: 4000000 INFO @ Sun, 21 Jun 2020 17:30:30: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:30:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:30:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:30:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:30:35: 6000000 INFO @ Sun, 21 Jun 2020 17:30:40: 1000000 INFO @ Sun, 21 Jun 2020 17:30:41: 7000000 INFO @ Sun, 21 Jun 2020 17:30:47: 2000000 INFO @ Sun, 21 Jun 2020 17:30:47: 8000000 INFO @ Sun, 21 Jun 2020 17:30:53: 9000000 INFO @ Sun, 21 Jun 2020 17:30:53: 3000000 INFO @ Sun, 21 Jun 2020 17:30:59: 10000000 INFO @ Sun, 21 Jun 2020 17:31:00: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:31:05: 11000000 INFO @ Sun, 21 Jun 2020 17:31:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:31:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:31:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:31:06: 5000000 INFO @ Sun, 21 Jun 2020 17:31:11: 12000000 INFO @ Sun, 21 Jun 2020 17:31:12: 1000000 INFO @ Sun, 21 Jun 2020 17:31:13: 6000000 INFO @ Sun, 21 Jun 2020 17:31:17: 13000000 INFO @ Sun, 21 Jun 2020 17:31:19: 2000000 INFO @ Sun, 21 Jun 2020 17:31:20: 7000000 INFO @ Sun, 21 Jun 2020 17:31:23: 14000000 INFO @ Sun, 21 Jun 2020 17:31:25: 3000000 INFO @ Sun, 21 Jun 2020 17:31:27: 8000000 INFO @ Sun, 21 Jun 2020 17:31:29: 15000000 INFO @ Sun, 21 Jun 2020 17:31:32: 4000000 INFO @ Sun, 21 Jun 2020 17:31:33: 9000000 INFO @ Sun, 21 Jun 2020 17:31:35: 16000000 INFO @ Sun, 21 Jun 2020 17:31:39: 5000000 INFO @ Sun, 21 Jun 2020 17:31:40: 10000000 INFO @ Sun, 21 Jun 2020 17:31:41: 17000000 INFO @ Sun, 21 Jun 2020 17:31:46: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:31:46: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:31:46: #1 total tags in treatment: 17788739 INFO @ Sun, 21 Jun 2020 17:31:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:31:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:31:46: 6000000 INFO @ Sun, 21 Jun 2020 17:31:46: #1 tags after filtering in treatment: 17788736 INFO @ Sun, 21 Jun 2020 17:31:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:31:46: #1 finished! INFO @ Sun, 21 Jun 2020 17:31:46: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:31:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:31:47: 11000000 INFO @ Sun, 21 Jun 2020 17:31:47: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 17:31:47: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:31:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:31:53: 7000000 INFO @ Sun, 21 Jun 2020 17:31:53: 12000000 INFO @ Sun, 21 Jun 2020 17:31:59: 8000000 INFO @ Sun, 21 Jun 2020 17:32:00: 13000000 INFO @ Sun, 21 Jun 2020 17:32:06: 9000000 INFO @ Sun, 21 Jun 2020 17:32:06: 14000000 INFO @ Sun, 21 Jun 2020 17:32:12: 10000000 INFO @ Sun, 21 Jun 2020 17:32:13: 15000000 INFO @ Sun, 21 Jun 2020 17:32:19: 11000000 INFO @ Sun, 21 Jun 2020 17:32:19: 16000000 INFO @ Sun, 21 Jun 2020 17:32:25: 12000000 INFO @ Sun, 21 Jun 2020 17:32:26: 17000000 INFO @ Sun, 21 Jun 2020 17:32:31: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:32:31: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:32:31: #1 total tags in treatment: 17788739 INFO @ Sun, 21 Jun 2020 17:32:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:32:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:32:32: #1 tags after filtering in treatment: 17788736 INFO @ Sun, 21 Jun 2020 17:32:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:32:32: #1 finished! INFO @ Sun, 21 Jun 2020 17:32:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:32:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:32:32: 13000000 INFO @ Sun, 21 Jun 2020 17:32:33: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 17:32:33: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:32:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:32:38: 14000000 INFO @ Sun, 21 Jun 2020 17:32:44: 15000000 INFO @ Sun, 21 Jun 2020 17:32:50: 16000000 INFO @ Sun, 21 Jun 2020 17:32:56: 17000000 INFO @ Sun, 21 Jun 2020 17:33:01: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:33:01: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:33:01: #1 total tags in treatment: 17788739 INFO @ Sun, 21 Jun 2020 17:33:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:33:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:33:02: #1 tags after filtering in treatment: 17788736 INFO @ Sun, 21 Jun 2020 17:33:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:33:02: #1 finished! INFO @ Sun, 21 Jun 2020 17:33:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:33:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:33:03: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 17:33:03: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:33:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111818/SRX111818.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。