Job ID = 6453348 SRX = SRX111817 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:22:32 prefetch.2.10.7: 1) Downloading 'SRR390282'... 2020-06-21T08:22:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:27:54 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:27:54 prefetch.2.10.7: 1) 'SRR390282' was downloaded successfully Read 20834747 spots for SRR390282/SRR390282.sra Written 20834747 spots for SRR390282/SRR390282.sra 2020-06-21T08:29:06 prefetch.2.10.7: 1) Downloading 'SRR390283'... 2020-06-21T08:29:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:30:55 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:30:56 prefetch.2.10.7: 'SRR390283' is valid 2020-06-21T08:30:56 prefetch.2.10.7: 1) 'SRR390283' was downloaded successfully Read 13623353 spots for SRR390283/SRR390283.sra Written 13623353 spots for SRR390283/SRR390283.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:59 34458100 reads; of these: 34458100 (100.00%) were unpaired; of these: 11988012 (34.79%) aligned 0 times 18386543 (53.36%) aligned exactly 1 time 4083545 (11.85%) aligned >1 times 65.21% overall alignment rate Time searching: 00:05:59 Overall time: 00:05:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5634859 / 22470088 = 0.2508 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:43:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:43:22: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:43:22: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:43:28: 1000000 INFO @ Sun, 21 Jun 2020 17:43:35: 2000000 INFO @ Sun, 21 Jun 2020 17:43:41: 3000000 INFO @ Sun, 21 Jun 2020 17:43:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:43:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:43:52: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:43:52: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:43:53: 5000000 INFO @ Sun, 21 Jun 2020 17:43:59: 1000000 INFO @ Sun, 21 Jun 2020 17:44:00: 6000000 INFO @ Sun, 21 Jun 2020 17:44:06: 2000000 INFO @ Sun, 21 Jun 2020 17:44:06: 7000000 INFO @ Sun, 21 Jun 2020 17:44:12: 3000000 INFO @ Sun, 21 Jun 2020 17:44:13: 8000000 INFO @ Sun, 21 Jun 2020 17:44:19: 4000000 INFO @ Sun, 21 Jun 2020 17:44:19: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:44:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:44:22: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:44:22: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:44:25: 5000000 INFO @ Sun, 21 Jun 2020 17:44:26: 10000000 INFO @ Sun, 21 Jun 2020 17:44:29: 1000000 INFO @ Sun, 21 Jun 2020 17:44:32: 6000000 INFO @ Sun, 21 Jun 2020 17:44:33: 11000000 INFO @ Sun, 21 Jun 2020 17:44:36: 2000000 INFO @ Sun, 21 Jun 2020 17:44:39: 7000000 INFO @ Sun, 21 Jun 2020 17:44:40: 12000000 INFO @ Sun, 21 Jun 2020 17:44:42: 3000000 INFO @ Sun, 21 Jun 2020 17:44:45: 8000000 INFO @ Sun, 21 Jun 2020 17:44:46: 13000000 INFO @ Sun, 21 Jun 2020 17:44:49: 4000000 INFO @ Sun, 21 Jun 2020 17:44:52: 9000000 INFO @ Sun, 21 Jun 2020 17:44:54: 14000000 INFO @ Sun, 21 Jun 2020 17:44:56: 5000000 INFO @ Sun, 21 Jun 2020 17:44:59: 10000000 INFO @ Sun, 21 Jun 2020 17:45:00: 15000000 INFO @ Sun, 21 Jun 2020 17:45:02: 6000000 INFO @ Sun, 21 Jun 2020 17:45:05: 11000000 INFO @ Sun, 21 Jun 2020 17:45:07: 16000000 INFO @ Sun, 21 Jun 2020 17:45:09: 7000000 INFO @ Sun, 21 Jun 2020 17:45:12: 12000000 INFO @ Sun, 21 Jun 2020 17:45:13: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:45:13: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:45:13: #1 total tags in treatment: 16835229 INFO @ Sun, 21 Jun 2020 17:45:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:45:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:45:13: #1 tags after filtering in treatment: 16835228 INFO @ Sun, 21 Jun 2020 17:45:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:45:13: #1 finished! INFO @ Sun, 21 Jun 2020 17:45:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:45:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:45:14: #2 number of paired peaks: 79 WARNING @ Sun, 21 Jun 2020 17:45:14: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:45:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:45:16: 8000000 INFO @ Sun, 21 Jun 2020 17:45:18: 13000000 INFO @ Sun, 21 Jun 2020 17:45:22: 9000000 INFO @ Sun, 21 Jun 2020 17:45:25: 14000000 INFO @ Sun, 21 Jun 2020 17:45:29: 10000000 INFO @ Sun, 21 Jun 2020 17:45:32: 15000000 INFO @ Sun, 21 Jun 2020 17:45:36: 11000000 INFO @ Sun, 21 Jun 2020 17:45:38: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:45:42: 12000000 INFO @ Sun, 21 Jun 2020 17:45:44: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:45:44: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:45:44: #1 total tags in treatment: 16835229 INFO @ Sun, 21 Jun 2020 17:45:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:45:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:45:44: #1 tags after filtering in treatment: 16835228 INFO @ Sun, 21 Jun 2020 17:45:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:45:44: #1 finished! INFO @ Sun, 21 Jun 2020 17:45:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:45:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:45:45: #2 number of paired peaks: 79 WARNING @ Sun, 21 Jun 2020 17:45:45: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:45:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:45:48: 13000000 INFO @ Sun, 21 Jun 2020 17:45:55: 14000000 INFO @ Sun, 21 Jun 2020 17:46:01: 15000000 INFO @ Sun, 21 Jun 2020 17:46:07: 16000000 INFO @ Sun, 21 Jun 2020 17:46:13: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:46:13: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:46:13: #1 total tags in treatment: 16835229 INFO @ Sun, 21 Jun 2020 17:46:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:46:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:46:13: #1 tags after filtering in treatment: 16835228 INFO @ Sun, 21 Jun 2020 17:46:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:46:13: #1 finished! INFO @ Sun, 21 Jun 2020 17:46:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:46:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:46:14: #2 number of paired peaks: 79 WARNING @ Sun, 21 Jun 2020 17:46:14: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:46:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111817/SRX111817.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。