Job ID = 6453345 SRX = SRX111814 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:25:41 prefetch.2.10.7: 1) Downloading 'SRR390277'... 2020-06-21T08:25:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:31:37 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:31:37 prefetch.2.10.7: 1) 'SRR390277' was downloaded successfully Read 23604057 spots for SRR390277/SRR390277.sra Written 23604057 spots for SRR390277/SRR390277.sra 2020-06-21T08:32:51 prefetch.2.10.7: 1) Downloading 'SRR390278'... 2020-06-21T08:32:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:37:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:37:26 prefetch.2.10.7: 1) 'SRR390278' was downloaded successfully Read 17978113 spots for SRR390278/SRR390278.sra Written 17978113 spots for SRR390278/SRR390278.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:58 41582170 reads; of these: 41582170 (100.00%) were unpaired; of these: 2847043 (6.85%) aligned 0 times 27000976 (64.93%) aligned exactly 1 time 11734151 (28.22%) aligned >1 times 93.15% overall alignment rate Time searching: 00:09:58 Overall time: 00:09:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14838370 / 38735127 = 0.3831 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:56:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:56:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:56:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:56:35: 1000000 INFO @ Sun, 21 Jun 2020 17:56:41: 2000000 INFO @ Sun, 21 Jun 2020 17:56:46: 3000000 INFO @ Sun, 21 Jun 2020 17:56:51: 4000000 INFO @ Sun, 21 Jun 2020 17:56:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:57:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:57:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:57:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:57:01: 6000000 INFO @ Sun, 21 Jun 2020 17:57:06: 1000000 INFO @ Sun, 21 Jun 2020 17:57:06: 7000000 INFO @ Sun, 21 Jun 2020 17:57:12: 2000000 INFO @ Sun, 21 Jun 2020 17:57:12: 8000000 INFO @ Sun, 21 Jun 2020 17:57:17: 3000000 INFO @ Sun, 21 Jun 2020 17:57:17: 9000000 INFO @ Sun, 21 Jun 2020 17:57:22: 4000000 INFO @ Sun, 21 Jun 2020 17:57:23: 10000000 INFO @ Sun, 21 Jun 2020 17:57:28: 5000000 INFO @ Sun, 21 Jun 2020 17:57:28: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:57:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:57:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:57:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:57:33: 6000000 INFO @ Sun, 21 Jun 2020 17:57:34: 12000000 INFO @ Sun, 21 Jun 2020 17:57:36: 1000000 INFO @ Sun, 21 Jun 2020 17:57:39: 7000000 INFO @ Sun, 21 Jun 2020 17:57:39: 13000000 INFO @ Sun, 21 Jun 2020 17:57:41: 2000000 INFO @ Sun, 21 Jun 2020 17:57:44: 8000000 INFO @ Sun, 21 Jun 2020 17:57:44: 14000000 INFO @ Sun, 21 Jun 2020 17:57:47: 3000000 INFO @ Sun, 21 Jun 2020 17:57:49: 9000000 INFO @ Sun, 21 Jun 2020 17:57:50: 15000000 INFO @ Sun, 21 Jun 2020 17:57:52: 4000000 INFO @ Sun, 21 Jun 2020 17:57:55: 10000000 INFO @ Sun, 21 Jun 2020 17:57:55: 16000000 INFO @ Sun, 21 Jun 2020 17:57:57: 5000000 INFO @ Sun, 21 Jun 2020 17:58:00: 11000000 INFO @ Sun, 21 Jun 2020 17:58:01: 17000000 INFO @ Sun, 21 Jun 2020 17:58:03: 6000000 INFO @ Sun, 21 Jun 2020 17:58:05: 12000000 INFO @ Sun, 21 Jun 2020 17:58:06: 18000000 INFO @ Sun, 21 Jun 2020 17:58:08: 7000000 INFO @ Sun, 21 Jun 2020 17:58:11: 13000000 INFO @ Sun, 21 Jun 2020 17:58:12: 19000000 INFO @ Sun, 21 Jun 2020 17:58:13: 8000000 INFO @ Sun, 21 Jun 2020 17:58:16: 14000000 INFO @ Sun, 21 Jun 2020 17:58:17: 20000000 INFO @ Sun, 21 Jun 2020 17:58:19: 9000000 INFO @ Sun, 21 Jun 2020 17:58:21: 15000000 INFO @ Sun, 21 Jun 2020 17:58:22: 21000000 INFO @ Sun, 21 Jun 2020 17:58:24: 10000000 INFO @ Sun, 21 Jun 2020 17:58:27: 16000000 INFO @ Sun, 21 Jun 2020 17:58:28: 22000000 INFO @ Sun, 21 Jun 2020 17:58:29: 11000000 INFO @ Sun, 21 Jun 2020 17:58:32: 17000000 INFO @ Sun, 21 Jun 2020 17:58:33: 23000000 INFO @ Sun, 21 Jun 2020 17:58:35: 12000000 INFO @ Sun, 21 Jun 2020 17:58:37: 18000000 INFO @ Sun, 21 Jun 2020 17:58:38: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:58:38: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:58:38: #1 total tags in treatment: 23896757 INFO @ Sun, 21 Jun 2020 17:58:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:58:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:58:39: #1 tags after filtering in treatment: 23896717 INFO @ Sun, 21 Jun 2020 17:58:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:58:39: #1 finished! INFO @ Sun, 21 Jun 2020 17:58:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:58:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:58:40: 13000000 INFO @ Sun, 21 Jun 2020 17:58:40: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 17:58:40: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:58:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:58:43: 19000000 INFO @ Sun, 21 Jun 2020 17:58:45: 14000000 INFO @ Sun, 21 Jun 2020 17:58:48: 20000000 INFO @ Sun, 21 Jun 2020 17:58:50: 15000000 INFO @ Sun, 21 Jun 2020 17:58:53: 21000000 INFO @ Sun, 21 Jun 2020 17:58:56: 16000000 INFO @ Sun, 21 Jun 2020 17:58:59: 22000000 INFO @ Sun, 21 Jun 2020 17:59:01: 17000000 INFO @ Sun, 21 Jun 2020 17:59:04: 23000000 INFO @ Sun, 21 Jun 2020 17:59:06: 18000000 INFO @ Sun, 21 Jun 2020 17:59:09: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:59:09: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:59:09: #1 total tags in treatment: 23896757 INFO @ Sun, 21 Jun 2020 17:59:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:59:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:59:10: #1 tags after filtering in treatment: 23896717 INFO @ Sun, 21 Jun 2020 17:59:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:59:10: #1 finished! INFO @ Sun, 21 Jun 2020 17:59:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:59:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:59:11: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 17:59:11: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:59:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:59:12: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:59:17: 20000000 INFO @ Sun, 21 Jun 2020 17:59:22: 21000000 INFO @ Sun, 21 Jun 2020 17:59:27: 22000000 INFO @ Sun, 21 Jun 2020 17:59:32: 23000000 INFO @ Sun, 21 Jun 2020 17:59:36: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:59:36: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:59:36: #1 total tags in treatment: 23896757 INFO @ Sun, 21 Jun 2020 17:59:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:59:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:59:37: #1 tags after filtering in treatment: 23896717 INFO @ Sun, 21 Jun 2020 17:59:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:59:37: #1 finished! INFO @ Sun, 21 Jun 2020 17:59:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:59:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:59:38: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 17:59:38: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:59:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111814/SRX111814.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。