Job ID = 6453336 SRX = SRX111808 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:19:03 prefetch.2.10.7: 1) Downloading 'SRR390268'... 2020-06-21T08:19:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:24:02 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:24:02 prefetch.2.10.7: 1) 'SRR390268' was downloaded successfully Read 20354253 spots for SRR390268/SRR390268.sra Written 20354253 spots for SRR390268/SRR390268.sra 2020-06-21T08:25:08 prefetch.2.10.7: 1) Downloading 'SRR390269'... 2020-06-21T08:25:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:28:50 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:28:50 prefetch.2.10.7: 'SRR390269' is valid 2020-06-21T08:28:50 prefetch.2.10.7: 1) 'SRR390269' was downloaded successfully Read 16715588 spots for SRR390269/SRR390269.sra Written 16715588 spots for SRR390269/SRR390269.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:43 37069841 reads; of these: 37069841 (100.00%) were unpaired; of these: 2262716 (6.10%) aligned 0 times 25065649 (67.62%) aligned exactly 1 time 9741476 (26.28%) aligned >1 times 93.90% overall alignment rate Time searching: 00:08:43 Overall time: 00:08:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12498228 / 34807125 = 0.3591 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:46:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:46:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:46:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:46:07: 1000000 INFO @ Sun, 21 Jun 2020 17:46:13: 2000000 INFO @ Sun, 21 Jun 2020 17:46:18: 3000000 INFO @ Sun, 21 Jun 2020 17:46:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:46:30: 5000000 INFO @ Sun, 21 Jun 2020 17:46:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:46:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:46:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:46:36: 6000000 INFO @ Sun, 21 Jun 2020 17:46:36: 1000000 INFO @ Sun, 21 Jun 2020 17:46:42: 7000000 INFO @ Sun, 21 Jun 2020 17:46:42: 2000000 INFO @ Sun, 21 Jun 2020 17:46:47: 3000000 INFO @ Sun, 21 Jun 2020 17:46:48: 8000000 INFO @ Sun, 21 Jun 2020 17:46:52: 4000000 INFO @ Sun, 21 Jun 2020 17:46:54: 9000000 INFO @ Sun, 21 Jun 2020 17:46:58: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:47:00: 10000000 INFO @ Sun, 21 Jun 2020 17:47:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:47:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:47:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:47:03: 6000000 INFO @ Sun, 21 Jun 2020 17:47:06: 11000000 INFO @ Sun, 21 Jun 2020 17:47:07: 1000000 INFO @ Sun, 21 Jun 2020 17:47:09: 7000000 INFO @ Sun, 21 Jun 2020 17:47:12: 12000000 INFO @ Sun, 21 Jun 2020 17:47:12: 2000000 INFO @ Sun, 21 Jun 2020 17:47:14: 8000000 INFO @ Sun, 21 Jun 2020 17:47:17: 3000000 INFO @ Sun, 21 Jun 2020 17:47:18: 13000000 INFO @ Sun, 21 Jun 2020 17:47:19: 9000000 INFO @ Sun, 21 Jun 2020 17:47:23: 4000000 INFO @ Sun, 21 Jun 2020 17:47:24: 14000000 INFO @ Sun, 21 Jun 2020 17:47:25: 10000000 INFO @ Sun, 21 Jun 2020 17:47:28: 5000000 INFO @ Sun, 21 Jun 2020 17:47:29: 15000000 INFO @ Sun, 21 Jun 2020 17:47:30: 11000000 INFO @ Sun, 21 Jun 2020 17:47:33: 6000000 INFO @ Sun, 21 Jun 2020 17:47:35: 12000000 INFO @ Sun, 21 Jun 2020 17:47:35: 16000000 INFO @ Sun, 21 Jun 2020 17:47:39: 7000000 INFO @ Sun, 21 Jun 2020 17:47:41: 13000000 INFO @ Sun, 21 Jun 2020 17:47:41: 17000000 INFO @ Sun, 21 Jun 2020 17:47:44: 8000000 INFO @ Sun, 21 Jun 2020 17:47:46: 14000000 INFO @ Sun, 21 Jun 2020 17:47:48: 18000000 INFO @ Sun, 21 Jun 2020 17:47:49: 9000000 INFO @ Sun, 21 Jun 2020 17:47:51: 15000000 INFO @ Sun, 21 Jun 2020 17:47:54: 19000000 INFO @ Sun, 21 Jun 2020 17:47:55: 10000000 INFO @ Sun, 21 Jun 2020 17:47:57: 16000000 INFO @ Sun, 21 Jun 2020 17:48:00: 20000000 INFO @ Sun, 21 Jun 2020 17:48:00: 11000000 INFO @ Sun, 21 Jun 2020 17:48:02: 17000000 INFO @ Sun, 21 Jun 2020 17:48:06: 12000000 INFO @ Sun, 21 Jun 2020 17:48:06: 21000000 INFO @ Sun, 21 Jun 2020 17:48:08: 18000000 INFO @ Sun, 21 Jun 2020 17:48:11: 13000000 INFO @ Sun, 21 Jun 2020 17:48:12: 22000000 INFO @ Sun, 21 Jun 2020 17:48:13: 19000000 INFO @ Sun, 21 Jun 2020 17:48:15: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:48:15: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:48:15: #1 total tags in treatment: 22308897 INFO @ Sun, 21 Jun 2020 17:48:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:48:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:48:15: #1 tags after filtering in treatment: 22308849 INFO @ Sun, 21 Jun 2020 17:48:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:48:15: #1 finished! INFO @ Sun, 21 Jun 2020 17:48:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:48:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:48:16: 14000000 INFO @ Sun, 21 Jun 2020 17:48:16: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 17:48:16: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:48:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:48:18: 20000000 INFO @ Sun, 21 Jun 2020 17:48:21: 15000000 INFO @ Sun, 21 Jun 2020 17:48:24: 21000000 INFO @ Sun, 21 Jun 2020 17:48:27: 16000000 INFO @ Sun, 21 Jun 2020 17:48:29: 22000000 INFO @ Sun, 21 Jun 2020 17:48:31: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:48:31: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:48:31: #1 total tags in treatment: 22308897 INFO @ Sun, 21 Jun 2020 17:48:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:48:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:48:32: 17000000 INFO @ Sun, 21 Jun 2020 17:48:32: #1 tags after filtering in treatment: 22308849 INFO @ Sun, 21 Jun 2020 17:48:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:48:32: #1 finished! INFO @ Sun, 21 Jun 2020 17:48:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:48:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:48:33: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 17:48:33: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:48:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:48:37: 18000000 INFO @ Sun, 21 Jun 2020 17:48:42: 19000000 INFO @ Sun, 21 Jun 2020 17:48:47: 20000000 INFO @ Sun, 21 Jun 2020 17:48:52: 21000000 INFO @ Sun, 21 Jun 2020 17:48:57: 22000000 INFO @ Sun, 21 Jun 2020 17:48:59: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:48:59: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:48:59: #1 total tags in treatment: 22308897 INFO @ Sun, 21 Jun 2020 17:48:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:48:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:49:00: #1 tags after filtering in treatment: 22308849 INFO @ Sun, 21 Jun 2020 17:49:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:49:00: #1 finished! INFO @ Sun, 21 Jun 2020 17:49:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:49:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:49:01: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 17:49:01: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:49:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111808/SRX111808.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。