Job ID = 6453323 SRX = SRX111798 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:20:17 prefetch.2.10.7: 1) Downloading 'SRR390253'... 2020-06-21T08:20:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:23:22 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:23:23 prefetch.2.10.7: 'SRR390253' is valid 2020-06-21T08:23:23 prefetch.2.10.7: 1) 'SRR390253' was downloaded successfully Read 17174148 spots for SRR390253/SRR390253.sra Written 17174148 spots for SRR390253/SRR390253.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:41 17174148 reads; of these: 17174148 (100.00%) were unpaired; of these: 283238 (1.65%) aligned 0 times 14347916 (83.54%) aligned exactly 1 time 2542994 (14.81%) aligned >1 times 98.35% overall alignment rate Time searching: 00:03:41 Overall time: 00:03:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3684159 / 16890910 = 0.2181 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:31:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:31:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:31:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:31:51: 1000000 INFO @ Sun, 21 Jun 2020 17:31:57: 2000000 INFO @ Sun, 21 Jun 2020 17:32:02: 3000000 INFO @ Sun, 21 Jun 2020 17:32:08: 4000000 INFO @ Sun, 21 Jun 2020 17:32:13: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:32:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:32:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:32:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:32:19: 6000000 INFO @ Sun, 21 Jun 2020 17:32:22: 1000000 INFO @ Sun, 21 Jun 2020 17:32:25: 7000000 INFO @ Sun, 21 Jun 2020 17:32:28: 2000000 INFO @ Sun, 21 Jun 2020 17:32:32: 8000000 INFO @ Sun, 21 Jun 2020 17:32:35: 3000000 INFO @ Sun, 21 Jun 2020 17:32:39: 9000000 INFO @ Sun, 21 Jun 2020 17:32:42: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:32:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:32:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:32:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:32:46: 10000000 INFO @ Sun, 21 Jun 2020 17:32:49: 5000000 INFO @ Sun, 21 Jun 2020 17:32:53: 1000000 INFO @ Sun, 21 Jun 2020 17:32:54: 11000000 INFO @ Sun, 21 Jun 2020 17:32:56: 6000000 INFO @ Sun, 21 Jun 2020 17:33:00: 2000000 INFO @ Sun, 21 Jun 2020 17:33:01: 12000000 INFO @ Sun, 21 Jun 2020 17:33:03: 7000000 INFO @ Sun, 21 Jun 2020 17:33:08: 3000000 INFO @ Sun, 21 Jun 2020 17:33:08: 13000000 INFO @ Sun, 21 Jun 2020 17:33:09: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:33:09: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:33:09: #1 total tags in treatment: 13206751 INFO @ Sun, 21 Jun 2020 17:33:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:33:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:33:10: 8000000 INFO @ Sun, 21 Jun 2020 17:33:10: #1 tags after filtering in treatment: 13206740 INFO @ Sun, 21 Jun 2020 17:33:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:33:10: #1 finished! INFO @ Sun, 21 Jun 2020 17:33:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:33:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:33:11: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 17:33:11: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:33:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:33:15: 4000000 INFO @ Sun, 21 Jun 2020 17:33:17: 9000000 INFO @ Sun, 21 Jun 2020 17:33:23: 5000000 INFO @ Sun, 21 Jun 2020 17:33:24: 10000000 INFO @ Sun, 21 Jun 2020 17:33:30: 6000000 INFO @ Sun, 21 Jun 2020 17:33:31: 11000000 INFO @ Sun, 21 Jun 2020 17:33:38: 7000000 INFO @ Sun, 21 Jun 2020 17:33:38: 12000000 INFO @ Sun, 21 Jun 2020 17:33:45: 8000000 INFO @ Sun, 21 Jun 2020 17:33:46: 13000000 INFO @ Sun, 21 Jun 2020 17:33:47: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:33:47: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:33:47: #1 total tags in treatment: 13206751 INFO @ Sun, 21 Jun 2020 17:33:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:33:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:33:48: #1 tags after filtering in treatment: 13206740 INFO @ Sun, 21 Jun 2020 17:33:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:33:48: #1 finished! INFO @ Sun, 21 Jun 2020 17:33:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:33:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:33:49: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 17:33:49: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:33:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:33:52: 9000000 INFO @ Sun, 21 Jun 2020 17:33:59: 10000000 INFO @ Sun, 21 Jun 2020 17:34:06: 11000000 INFO @ Sun, 21 Jun 2020 17:34:13: 12000000 INFO @ Sun, 21 Jun 2020 17:34:19: 13000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 17:34:21: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:34:21: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:34:21: #1 total tags in treatment: 13206751 INFO @ Sun, 21 Jun 2020 17:34:21: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:34:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:34:21: #1 tags after filtering in treatment: 13206740 INFO @ Sun, 21 Jun 2020 17:34:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:34:21: #1 finished! INFO @ Sun, 21 Jun 2020 17:34:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:34:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:34:22: #2 number of paired peaks: 54 WARNING @ Sun, 21 Jun 2020 17:34:22: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:34:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111798/SRX111798.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling