Job ID = 6453322 SRX = SRX111797 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:11:33 prefetch.2.10.7: 1) Downloading 'SRR390251'... 2020-06-21T08:11:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:14:12 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:14:12 prefetch.2.10.7: 'SRR390251' is valid 2020-06-21T08:14:13 prefetch.2.10.7: 1) 'SRR390251' was downloaded successfully Read 16509025 spots for SRR390251/SRR390251.sra Written 16509025 spots for SRR390251/SRR390251.sra 2020-06-21T08:15:08 prefetch.2.10.7: 1) Downloading 'SRR390252'... 2020-06-21T08:15:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:23:59 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:23:59 prefetch.2.10.7: 1) 'SRR390252' was downloaded successfully Read 32217114 spots for SRR390252/SRR390252.sra Written 32217114 spots for SRR390252/SRR390252.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:02 48726139 reads; of these: 48726139 (100.00%) were unpaired; of these: 12724460 (26.11%) aligned 0 times 31403197 (64.45%) aligned exactly 1 time 4598482 (9.44%) aligned >1 times 73.89% overall alignment rate Time searching: 00:08:02 Overall time: 00:08:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11466215 / 36001679 = 0.3185 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:41:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:41:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:41:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:41:29: 1000000 INFO @ Sun, 21 Jun 2020 17:41:35: 2000000 INFO @ Sun, 21 Jun 2020 17:41:40: 3000000 INFO @ Sun, 21 Jun 2020 17:41:46: 4000000 INFO @ Sun, 21 Jun 2020 17:41:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:41:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:41:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:41:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:41:58: 6000000 INFO @ Sun, 21 Jun 2020 17:42:02: 1000000 INFO @ Sun, 21 Jun 2020 17:42:04: 7000000 INFO @ Sun, 21 Jun 2020 17:42:09: 2000000 INFO @ Sun, 21 Jun 2020 17:42:11: 8000000 INFO @ Sun, 21 Jun 2020 17:42:17: 3000000 INFO @ Sun, 21 Jun 2020 17:42:17: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:42:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:42:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:42:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:42:24: 4000000 INFO @ Sun, 21 Jun 2020 17:42:24: 10000000 INFO @ Sun, 21 Jun 2020 17:42:31: 1000000 INFO @ Sun, 21 Jun 2020 17:42:31: 11000000 INFO @ Sun, 21 Jun 2020 17:42:32: 5000000 INFO @ Sun, 21 Jun 2020 17:42:38: 2000000 INFO @ Sun, 21 Jun 2020 17:42:38: 12000000 INFO @ Sun, 21 Jun 2020 17:42:39: 6000000 INFO @ Sun, 21 Jun 2020 17:42:45: 3000000 INFO @ Sun, 21 Jun 2020 17:42:45: 13000000 INFO @ Sun, 21 Jun 2020 17:42:47: 7000000 INFO @ Sun, 21 Jun 2020 17:42:52: 4000000 INFO @ Sun, 21 Jun 2020 17:42:52: 14000000 INFO @ Sun, 21 Jun 2020 17:42:54: 8000000 INFO @ Sun, 21 Jun 2020 17:42:59: 5000000 INFO @ Sun, 21 Jun 2020 17:42:59: 15000000 INFO @ Sun, 21 Jun 2020 17:43:01: 9000000 INFO @ Sun, 21 Jun 2020 17:43:06: 6000000 INFO @ Sun, 21 Jun 2020 17:43:06: 16000000 INFO @ Sun, 21 Jun 2020 17:43:09: 10000000 INFO @ Sun, 21 Jun 2020 17:43:13: 7000000 INFO @ Sun, 21 Jun 2020 17:43:13: 17000000 INFO @ Sun, 21 Jun 2020 17:43:17: 11000000 INFO @ Sun, 21 Jun 2020 17:43:20: 8000000 INFO @ Sun, 21 Jun 2020 17:43:20: 18000000 INFO @ Sun, 21 Jun 2020 17:43:25: 12000000 INFO @ Sun, 21 Jun 2020 17:43:27: 9000000 INFO @ Sun, 21 Jun 2020 17:43:28: 19000000 INFO @ Sun, 21 Jun 2020 17:43:32: 13000000 INFO @ Sun, 21 Jun 2020 17:43:34: 10000000 INFO @ Sun, 21 Jun 2020 17:43:35: 20000000 INFO @ Sun, 21 Jun 2020 17:43:40: 14000000 INFO @ Sun, 21 Jun 2020 17:43:41: 11000000 INFO @ Sun, 21 Jun 2020 17:43:43: 21000000 INFO @ Sun, 21 Jun 2020 17:43:48: 15000000 INFO @ Sun, 21 Jun 2020 17:43:48: 12000000 INFO @ Sun, 21 Jun 2020 17:43:50: 22000000 INFO @ Sun, 21 Jun 2020 17:43:55: 13000000 INFO @ Sun, 21 Jun 2020 17:43:56: 16000000 INFO @ Sun, 21 Jun 2020 17:43:58: 23000000 INFO @ Sun, 21 Jun 2020 17:44:03: 14000000 INFO @ Sun, 21 Jun 2020 17:44:04: 17000000 INFO @ Sun, 21 Jun 2020 17:44:05: 24000000 INFO @ Sun, 21 Jun 2020 17:44:09: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:44:09: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:44:09: #1 total tags in treatment: 24535464 INFO @ Sun, 21 Jun 2020 17:44:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:44:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:44:10: 15000000 INFO @ Sun, 21 Jun 2020 17:44:10: #1 tags after filtering in treatment: 24535462 INFO @ Sun, 21 Jun 2020 17:44:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:44:10: #1 finished! INFO @ Sun, 21 Jun 2020 17:44:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:44:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:44:11: 18000000 INFO @ Sun, 21 Jun 2020 17:44:12: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 17:44:12: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:44:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:44:17: 16000000 INFO @ Sun, 21 Jun 2020 17:44:19: 19000000 INFO @ Sun, 21 Jun 2020 17:44:24: 17000000 INFO @ Sun, 21 Jun 2020 17:44:27: 20000000 INFO @ Sun, 21 Jun 2020 17:44:31: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:44:35: 21000000 INFO @ Sun, 21 Jun 2020 17:44:38: 19000000 INFO @ Sun, 21 Jun 2020 17:44:43: 22000000 INFO @ Sun, 21 Jun 2020 17:44:45: 20000000 INFO @ Sun, 21 Jun 2020 17:44:50: 23000000 INFO @ Sun, 21 Jun 2020 17:44:52: 21000000 INFO @ Sun, 21 Jun 2020 17:44:58: 24000000 INFO @ Sun, 21 Jun 2020 17:44:59: 22000000 INFO @ Sun, 21 Jun 2020 17:45:03: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:45:03: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:45:03: #1 total tags in treatment: 24535464 INFO @ Sun, 21 Jun 2020 17:45:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:45:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:45:03: #1 tags after filtering in treatment: 24535462 INFO @ Sun, 21 Jun 2020 17:45:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:45:03: #1 finished! INFO @ Sun, 21 Jun 2020 17:45:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:45:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:45:05: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 17:45:05: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:45:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:45:06: 23000000 INFO @ Sun, 21 Jun 2020 17:45:12: 24000000 INFO @ Sun, 21 Jun 2020 17:45:16: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:45:16: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:45:16: #1 total tags in treatment: 24535464 INFO @ Sun, 21 Jun 2020 17:45:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:45:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:45:16: #1 tags after filtering in treatment: 24535462 INFO @ Sun, 21 Jun 2020 17:45:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:45:16: #1 finished! INFO @ Sun, 21 Jun 2020 17:45:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:45:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:45:18: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 17:45:18: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:45:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111797/SRX111797.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。