Job ID = 6453321 SRX = SRX111796 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:14:52 prefetch.2.10.7: 1) Downloading 'SRR390250'... 2020-06-21T08:14:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:17:33 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:17:33 prefetch.2.10.7: 1) 'SRR390250' was downloaded successfully Read 18246387 spots for SRR390250/SRR390250.sra Written 18246387 spots for SRR390250/SRR390250.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:50 18246387 reads; of these: 18246387 (100.00%) were unpaired; of these: 249033 (1.36%) aligned 0 times 15129918 (82.92%) aligned exactly 1 time 2867436 (15.72%) aligned >1 times 98.64% overall alignment rate Time searching: 00:03:50 Overall time: 00:03:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4044274 / 17997354 = 0.2247 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:26:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:26:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:26:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:26:34: 1000000 INFO @ Sun, 21 Jun 2020 17:26:39: 2000000 INFO @ Sun, 21 Jun 2020 17:26:44: 3000000 INFO @ Sun, 21 Jun 2020 17:26:48: 4000000 INFO @ Sun, 21 Jun 2020 17:26:53: 5000000 INFO @ Sun, 21 Jun 2020 17:26:58: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:27:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:27:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:27:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:27:03: 7000000 INFO @ Sun, 21 Jun 2020 17:27:05: 1000000 INFO @ Sun, 21 Jun 2020 17:27:08: 8000000 INFO @ Sun, 21 Jun 2020 17:27:10: 2000000 INFO @ Sun, 21 Jun 2020 17:27:13: 9000000 INFO @ Sun, 21 Jun 2020 17:27:14: 3000000 INFO @ Sun, 21 Jun 2020 17:27:18: 10000000 INFO @ Sun, 21 Jun 2020 17:27:19: 4000000 INFO @ Sun, 21 Jun 2020 17:27:23: 11000000 INFO @ Sun, 21 Jun 2020 17:27:24: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:27:28: 12000000 INFO @ Sun, 21 Jun 2020 17:27:29: 6000000 INFO @ Sun, 21 Jun 2020 17:27:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:27:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:27:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:27:33: 13000000 INFO @ Sun, 21 Jun 2020 17:27:34: 7000000 INFO @ Sun, 21 Jun 2020 17:27:35: 1000000 INFO @ Sun, 21 Jun 2020 17:27:38: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:27:38: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:27:38: #1 total tags in treatment: 13953080 INFO @ Sun, 21 Jun 2020 17:27:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:27:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:27:39: #1 tags after filtering in treatment: 13953075 INFO @ Sun, 21 Jun 2020 17:27:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:27:39: #1 finished! INFO @ Sun, 21 Jun 2020 17:27:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:27:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:27:39: 8000000 INFO @ Sun, 21 Jun 2020 17:27:39: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 17:27:39: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:27:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:27:40: 2000000 INFO @ Sun, 21 Jun 2020 17:27:44: 9000000 INFO @ Sun, 21 Jun 2020 17:27:45: 3000000 INFO @ Sun, 21 Jun 2020 17:27:49: 10000000 INFO @ Sun, 21 Jun 2020 17:27:50: 4000000 INFO @ Sun, 21 Jun 2020 17:27:54: 11000000 INFO @ Sun, 21 Jun 2020 17:27:55: 5000000 INFO @ Sun, 21 Jun 2020 17:28:00: 6000000 INFO @ Sun, 21 Jun 2020 17:28:00: 12000000 INFO @ Sun, 21 Jun 2020 17:28:05: 7000000 INFO @ Sun, 21 Jun 2020 17:28:05: 13000000 INFO @ Sun, 21 Jun 2020 17:28:09: 8000000 INFO @ Sun, 21 Jun 2020 17:28:10: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:28:10: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:28:10: #1 total tags in treatment: 13953080 INFO @ Sun, 21 Jun 2020 17:28:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:28:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:28:10: #1 tags after filtering in treatment: 13953075 INFO @ Sun, 21 Jun 2020 17:28:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:28:10: #1 finished! INFO @ Sun, 21 Jun 2020 17:28:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:28:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:28:11: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 17:28:11: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:28:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:28:14: 9000000 INFO @ Sun, 21 Jun 2020 17:28:19: 10000000 INFO @ Sun, 21 Jun 2020 17:28:24: 11000000 INFO @ Sun, 21 Jun 2020 17:28:29: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:28:34: 13000000 INFO @ Sun, 21 Jun 2020 17:28:39: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:28:39: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:28:39: #1 total tags in treatment: 13953080 INFO @ Sun, 21 Jun 2020 17:28:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:28:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:28:39: #1 tags after filtering in treatment: 13953075 INFO @ Sun, 21 Jun 2020 17:28:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:28:39: #1 finished! INFO @ Sun, 21 Jun 2020 17:28:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:28:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:28:40: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 17:28:40: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:28:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111796/SRX111796.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。