Job ID = 6453319 SRX = SRX111794 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:26:12 prefetch.2.10.7: 1) Downloading 'SRR390247'... 2020-06-21T08:26:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:29:21 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:29:22 prefetch.2.10.7: 'SRR390247' is valid 2020-06-21T08:29:22 prefetch.2.10.7: 1) 'SRR390247' was downloaded successfully Read 16750056 spots for SRR390247/SRR390247.sra Written 16750056 spots for SRR390247/SRR390247.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:24 16750056 reads; of these: 16750056 (100.00%) were unpaired; of these: 286882 (1.71%) aligned 0 times 13914485 (83.07%) aligned exactly 1 time 2548689 (15.22%) aligned >1 times 98.29% overall alignment rate Time searching: 00:03:24 Overall time: 00:03:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3760939 / 16463174 = 0.2284 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:37:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:37:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:37:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:37:47: 1000000 INFO @ Sun, 21 Jun 2020 17:37:52: 2000000 INFO @ Sun, 21 Jun 2020 17:37:57: 3000000 INFO @ Sun, 21 Jun 2020 17:38:03: 4000000 INFO @ Sun, 21 Jun 2020 17:38:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:38:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:38:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:38:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:38:14: 6000000 INFO @ Sun, 21 Jun 2020 17:38:17: 1000000 INFO @ Sun, 21 Jun 2020 17:38:20: 7000000 INFO @ Sun, 21 Jun 2020 17:38:23: 2000000 INFO @ Sun, 21 Jun 2020 17:38:26: 8000000 INFO @ Sun, 21 Jun 2020 17:38:29: 3000000 INFO @ Sun, 21 Jun 2020 17:38:32: 9000000 INFO @ Sun, 21 Jun 2020 17:38:35: 4000000 INFO @ Sun, 21 Jun 2020 17:38:38: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:38:41: 5000000 INFO @ Sun, 21 Jun 2020 17:38:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:38:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:38:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:38:44: 11000000 INFO @ Sun, 21 Jun 2020 17:38:47: 6000000 INFO @ Sun, 21 Jun 2020 17:38:47: 1000000 INFO @ Sun, 21 Jun 2020 17:38:50: 12000000 INFO @ Sun, 21 Jun 2020 17:38:53: 7000000 INFO @ Sun, 21 Jun 2020 17:38:53: 2000000 INFO @ Sun, 21 Jun 2020 17:38:55: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:38:55: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:38:55: #1 total tags in treatment: 12702235 INFO @ Sun, 21 Jun 2020 17:38:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:38:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:38:55: #1 tags after filtering in treatment: 12702213 INFO @ Sun, 21 Jun 2020 17:38:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:38:55: #1 finished! INFO @ Sun, 21 Jun 2020 17:38:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:38:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:38:56: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 17:38:56: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:38:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:38:59: 8000000 INFO @ Sun, 21 Jun 2020 17:38:59: 3000000 INFO @ Sun, 21 Jun 2020 17:39:05: 9000000 INFO @ Sun, 21 Jun 2020 17:39:05: 4000000 INFO @ Sun, 21 Jun 2020 17:39:11: 10000000 INFO @ Sun, 21 Jun 2020 17:39:11: 5000000 INFO @ Sun, 21 Jun 2020 17:39:17: 6000000 INFO @ Sun, 21 Jun 2020 17:39:18: 11000000 INFO @ Sun, 21 Jun 2020 17:39:23: 7000000 INFO @ Sun, 21 Jun 2020 17:39:24: 12000000 INFO @ Sun, 21 Jun 2020 17:39:28: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:39:28: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:39:28: #1 total tags in treatment: 12702235 INFO @ Sun, 21 Jun 2020 17:39:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:39:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:39:29: #1 tags after filtering in treatment: 12702213 INFO @ Sun, 21 Jun 2020 17:39:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:39:29: #1 finished! INFO @ Sun, 21 Jun 2020 17:39:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:39:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:39:29: 8000000 INFO @ Sun, 21 Jun 2020 17:39:29: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 17:39:29: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:39:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:39:35: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:39:41: 10000000 INFO @ Sun, 21 Jun 2020 17:39:46: 11000000 INFO @ Sun, 21 Jun 2020 17:39:52: 12000000 INFO @ Sun, 21 Jun 2020 17:39:56: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:39:56: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:39:56: #1 total tags in treatment: 12702235 INFO @ Sun, 21 Jun 2020 17:39:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:39:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:39:56: #1 tags after filtering in treatment: 12702213 INFO @ Sun, 21 Jun 2020 17:39:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:39:56: #1 finished! INFO @ Sun, 21 Jun 2020 17:39:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:39:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:39:57: #2 number of paired peaks: 25 WARNING @ Sun, 21 Jun 2020 17:39:57: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:39:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111794/SRX111794.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。