Job ID = 6453314 SRX = SRX111789 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:11:34 prefetch.2.10.7: 1) Downloading 'SRR390239'... 2020-06-21T08:11:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:15:32 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:15:32 prefetch.2.10.7: 1) 'SRR390239' was downloaded successfully Read 25673993 spots for SRR390239/SRR390239.sra Written 25673993 spots for SRR390239/SRR390239.sra 2020-06-21T08:16:52 prefetch.2.10.7: 1) Downloading 'SRR390240'... 2020-06-21T08:16:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:20:31 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:20:31 prefetch.2.10.7: 1) 'SRR390240' was downloaded successfully Read 20946942 spots for SRR390240/SRR390240.sra Written 20946942 spots for SRR390240/SRR390240.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:23 46620935 reads; of these: 46620935 (100.00%) were unpaired; of these: 5805557 (12.45%) aligned 0 times 32916315 (70.60%) aligned exactly 1 time 7899063 (16.94%) aligned >1 times 87.55% overall alignment rate Time searching: 00:09:23 Overall time: 00:09:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14474443 / 40815378 = 0.3546 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:39:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:39:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:39:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:39:44: 1000000 INFO @ Sun, 21 Jun 2020 17:39:50: 2000000 INFO @ Sun, 21 Jun 2020 17:39:55: 3000000 INFO @ Sun, 21 Jun 2020 17:40:00: 4000000 INFO @ Sun, 21 Jun 2020 17:40:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:40:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:40:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:40:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:40:11: 6000000 INFO @ Sun, 21 Jun 2020 17:40:15: 1000000 INFO @ Sun, 21 Jun 2020 17:40:17: 7000000 INFO @ Sun, 21 Jun 2020 17:40:22: 2000000 INFO @ Sun, 21 Jun 2020 17:40:23: 8000000 INFO @ Sun, 21 Jun 2020 17:40:29: 3000000 INFO @ Sun, 21 Jun 2020 17:40:30: 9000000 INFO @ Sun, 21 Jun 2020 17:40:36: 4000000 INFO @ Sun, 21 Jun 2020 17:40:36: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:40:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:40:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:40:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:40:42: 11000000 INFO @ Sun, 21 Jun 2020 17:40:42: 5000000 INFO @ Sun, 21 Jun 2020 17:40:45: 1000000 INFO @ Sun, 21 Jun 2020 17:40:48: 12000000 INFO @ Sun, 21 Jun 2020 17:40:49: 6000000 INFO @ Sun, 21 Jun 2020 17:40:52: 2000000 INFO @ Sun, 21 Jun 2020 17:40:55: 13000000 INFO @ Sun, 21 Jun 2020 17:40:56: 7000000 INFO @ Sun, 21 Jun 2020 17:40:58: 3000000 INFO @ Sun, 21 Jun 2020 17:41:01: 14000000 INFO @ Sun, 21 Jun 2020 17:41:03: 8000000 INFO @ Sun, 21 Jun 2020 17:41:05: 4000000 INFO @ Sun, 21 Jun 2020 17:41:07: 15000000 INFO @ Sun, 21 Jun 2020 17:41:10: 9000000 INFO @ Sun, 21 Jun 2020 17:41:11: 5000000 INFO @ Sun, 21 Jun 2020 17:41:14: 16000000 INFO @ Sun, 21 Jun 2020 17:41:17: 10000000 INFO @ Sun, 21 Jun 2020 17:41:18: 6000000 INFO @ Sun, 21 Jun 2020 17:41:20: 17000000 INFO @ Sun, 21 Jun 2020 17:41:23: 11000000 INFO @ Sun, 21 Jun 2020 17:41:24: 7000000 INFO @ Sun, 21 Jun 2020 17:41:26: 18000000 INFO @ Sun, 21 Jun 2020 17:41:30: 12000000 INFO @ Sun, 21 Jun 2020 17:41:31: 8000000 INFO @ Sun, 21 Jun 2020 17:41:33: 19000000 INFO @ Sun, 21 Jun 2020 17:41:37: 13000000 INFO @ Sun, 21 Jun 2020 17:41:37: 9000000 INFO @ Sun, 21 Jun 2020 17:41:39: 20000000 INFO @ Sun, 21 Jun 2020 17:41:43: 14000000 INFO @ Sun, 21 Jun 2020 17:41:44: 10000000 INFO @ Sun, 21 Jun 2020 17:41:45: 21000000 INFO @ Sun, 21 Jun 2020 17:41:50: 15000000 INFO @ Sun, 21 Jun 2020 17:41:50: 11000000 INFO @ Sun, 21 Jun 2020 17:41:52: 22000000 INFO @ Sun, 21 Jun 2020 17:41:57: 12000000 INFO @ Sun, 21 Jun 2020 17:41:57: 16000000 INFO @ Sun, 21 Jun 2020 17:41:58: 23000000 INFO @ Sun, 21 Jun 2020 17:42:03: 13000000 INFO @ Sun, 21 Jun 2020 17:42:04: 17000000 INFO @ Sun, 21 Jun 2020 17:42:05: 24000000 INFO @ Sun, 21 Jun 2020 17:42:10: 14000000 INFO @ Sun, 21 Jun 2020 17:42:11: 18000000 INFO @ Sun, 21 Jun 2020 17:42:11: 25000000 INFO @ Sun, 21 Jun 2020 17:42:16: 15000000 INFO @ Sun, 21 Jun 2020 17:42:17: 26000000 INFO @ Sun, 21 Jun 2020 17:42:18: 19000000 INFO @ Sun, 21 Jun 2020 17:42:20: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:42:20: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:42:20: #1 total tags in treatment: 26340935 INFO @ Sun, 21 Jun 2020 17:42:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:42:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:42:21: #1 tags after filtering in treatment: 26340935 INFO @ Sun, 21 Jun 2020 17:42:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:42:21: #1 finished! INFO @ Sun, 21 Jun 2020 17:42:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:42:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:42:22: #2 number of paired peaks: 30 WARNING @ Sun, 21 Jun 2020 17:42:22: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:42:22: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 17:42:22: 16000000 cut: /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:42:24: 20000000 INFO @ Sun, 21 Jun 2020 17:42:28: 17000000 INFO @ Sun, 21 Jun 2020 17:42:31: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:42:34: 18000000 INFO @ Sun, 21 Jun 2020 17:42:38: 22000000 INFO @ Sun, 21 Jun 2020 17:42:41: 19000000 INFO @ Sun, 21 Jun 2020 17:42:45: 23000000 INFO @ Sun, 21 Jun 2020 17:42:47: 20000000 INFO @ Sun, 21 Jun 2020 17:42:51: 24000000 INFO @ Sun, 21 Jun 2020 17:42:53: 21000000 INFO @ Sun, 21 Jun 2020 17:42:58: 25000000 INFO @ Sun, 21 Jun 2020 17:42:59: 22000000 INFO @ Sun, 21 Jun 2020 17:43:04: 26000000 INFO @ Sun, 21 Jun 2020 17:43:05: 23000000 INFO @ Sun, 21 Jun 2020 17:43:07: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:43:07: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:43:07: #1 total tags in treatment: 26340935 INFO @ Sun, 21 Jun 2020 17:43:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:43:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:43:08: #1 tags after filtering in treatment: 26340935 INFO @ Sun, 21 Jun 2020 17:43:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:43:08: #1 finished! INFO @ Sun, 21 Jun 2020 17:43:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:43:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:43:09: #2 number of paired peaks: 30 WARNING @ Sun, 21 Jun 2020 17:43:09: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:43:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:43:11: 24000000 INFO @ Sun, 21 Jun 2020 17:43:16: 25000000 INFO @ Sun, 21 Jun 2020 17:43:22: 26000000 INFO @ Sun, 21 Jun 2020 17:43:24: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:43:24: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:43:24: #1 total tags in treatment: 26340935 INFO @ Sun, 21 Jun 2020 17:43:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:43:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:43:24: #1 tags after filtering in treatment: 26340935 INFO @ Sun, 21 Jun 2020 17:43:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:43:24: #1 finished! INFO @ Sun, 21 Jun 2020 17:43:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:43:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:43:26: #2 number of paired peaks: 30 WARNING @ Sun, 21 Jun 2020 17:43:26: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:43:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111789/SRX111789.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。