Job ID = 6453310 SRX = SRX111787 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:27:26 prefetch.2.10.7: 1) Downloading 'SRR390236'... 2020-06-21T08:27:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:29:47 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:29:48 prefetch.2.10.7: 'SRR390236' is valid 2020-06-21T08:29:48 prefetch.2.10.7: 1) 'SRR390236' was downloaded successfully Read 19845454 spots for SRR390236/SRR390236.sra Written 19845454 spots for SRR390236/SRR390236.sra 2020-06-21T08:31:05 prefetch.2.10.7: 1) Downloading 'SRR390237'... 2020-06-21T08:31:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:34:19 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:34:19 prefetch.2.10.7: 1) 'SRR390237' was downloaded successfully Read 22516956 spots for SRR390237/SRR390237.sra Written 22516956 spots for SRR390237/SRR390237.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:59 42362410 reads; of these: 42362410 (100.00%) were unpaired; of these: 6989203 (16.50%) aligned 0 times 28815254 (68.02%) aligned exactly 1 time 6557953 (15.48%) aligned >1 times 83.50% overall alignment rate Time searching: 00:07:59 Overall time: 00:07:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12424384 / 35373207 = 0.3512 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:53:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:53:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:53:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:53:22: 1000000 INFO @ Sun, 21 Jun 2020 17:53:28: 2000000 INFO @ Sun, 21 Jun 2020 17:53:34: 3000000 INFO @ Sun, 21 Jun 2020 17:53:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:53:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:53:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:53:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:53:47: 5000000 INFO @ Sun, 21 Jun 2020 17:53:51: 1000000 INFO @ Sun, 21 Jun 2020 17:53:53: 6000000 INFO @ Sun, 21 Jun 2020 17:53:56: 2000000 INFO @ Sun, 21 Jun 2020 17:53:59: 7000000 INFO @ Sun, 21 Jun 2020 17:54:02: 3000000 INFO @ Sun, 21 Jun 2020 17:54:06: 8000000 INFO @ Sun, 21 Jun 2020 17:54:07: 4000000 INFO @ Sun, 21 Jun 2020 17:54:12: 9000000 INFO @ Sun, 21 Jun 2020 17:54:13: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:54:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:54:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:54:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:54:19: 10000000 INFO @ Sun, 21 Jun 2020 17:54:19: 6000000 INFO @ Sun, 21 Jun 2020 17:54:22: 1000000 INFO @ Sun, 21 Jun 2020 17:54:25: 7000000 INFO @ Sun, 21 Jun 2020 17:54:25: 11000000 INFO @ Sun, 21 Jun 2020 17:54:28: 2000000 INFO @ Sun, 21 Jun 2020 17:54:31: 8000000 INFO @ Sun, 21 Jun 2020 17:54:31: 12000000 INFO @ Sun, 21 Jun 2020 17:54:34: 3000000 INFO @ Sun, 21 Jun 2020 17:54:37: 9000000 INFO @ Sun, 21 Jun 2020 17:54:38: 13000000 INFO @ Sun, 21 Jun 2020 17:54:41: 4000000 INFO @ Sun, 21 Jun 2020 17:54:43: 10000000 INFO @ Sun, 21 Jun 2020 17:54:44: 14000000 INFO @ Sun, 21 Jun 2020 17:54:47: 5000000 INFO @ Sun, 21 Jun 2020 17:54:50: 11000000 INFO @ Sun, 21 Jun 2020 17:54:51: 15000000 INFO @ Sun, 21 Jun 2020 17:54:53: 6000000 INFO @ Sun, 21 Jun 2020 17:54:56: 12000000 INFO @ Sun, 21 Jun 2020 17:54:57: 16000000 INFO @ Sun, 21 Jun 2020 17:54:59: 7000000 INFO @ Sun, 21 Jun 2020 17:55:02: 13000000 INFO @ Sun, 21 Jun 2020 17:55:03: 17000000 INFO @ Sun, 21 Jun 2020 17:55:05: 8000000 INFO @ Sun, 21 Jun 2020 17:55:08: 14000000 INFO @ Sun, 21 Jun 2020 17:55:10: 18000000 INFO @ Sun, 21 Jun 2020 17:55:11: 9000000 INFO @ Sun, 21 Jun 2020 17:55:14: 15000000 INFO @ Sun, 21 Jun 2020 17:55:17: 19000000 INFO @ Sun, 21 Jun 2020 17:55:18: 10000000 INFO @ Sun, 21 Jun 2020 17:55:20: 16000000 INFO @ Sun, 21 Jun 2020 17:55:24: 20000000 INFO @ Sun, 21 Jun 2020 17:55:24: 11000000 INFO @ Sun, 21 Jun 2020 17:55:27: 17000000 INFO @ Sun, 21 Jun 2020 17:55:30: 12000000 INFO @ Sun, 21 Jun 2020 17:55:30: 21000000 INFO @ Sun, 21 Jun 2020 17:55:33: 18000000 INFO @ Sun, 21 Jun 2020 17:55:36: 13000000 INFO @ Sun, 21 Jun 2020 17:55:37: 22000000 INFO @ Sun, 21 Jun 2020 17:55:40: 19000000 INFO @ Sun, 21 Jun 2020 17:55:42: 14000000 INFO @ Sun, 21 Jun 2020 17:55:43: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:55:43: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:55:43: #1 total tags in treatment: 22948823 INFO @ Sun, 21 Jun 2020 17:55:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:55:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:55:44: #1 tags after filtering in treatment: 22948822 INFO @ Sun, 21 Jun 2020 17:55:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:55:44: #1 finished! INFO @ Sun, 21 Jun 2020 17:55:44: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:55:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:55:46: #2 number of paired peaks: 56 WARNING @ Sun, 21 Jun 2020 17:55:46: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:55:46: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 17:55:46: 20000000 cut: /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Sun, 21 Jun 2020 17:55:48: 15000000 INFO @ Sun, 21 Jun 2020 17:55:52: 21000000 INFO @ Sun, 21 Jun 2020 17:55:54: 16000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:55:58: 22000000 INFO @ Sun, 21 Jun 2020 17:56:00: 17000000 INFO @ Sun, 21 Jun 2020 17:56:04: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:56:04: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:56:04: #1 total tags in treatment: 22948823 INFO @ Sun, 21 Jun 2020 17:56:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:56:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:56:05: #1 tags after filtering in treatment: 22948822 INFO @ Sun, 21 Jun 2020 17:56:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:56:05: #1 finished! INFO @ Sun, 21 Jun 2020 17:56:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:56:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:56:06: 18000000 INFO @ Sun, 21 Jun 2020 17:56:07: #2 number of paired peaks: 56 WARNING @ Sun, 21 Jun 2020 17:56:07: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:56:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:56:12: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:56:17: 20000000 INFO @ Sun, 21 Jun 2020 17:56:23: 21000000 INFO @ Sun, 21 Jun 2020 17:56:28: 22000000 INFO @ Sun, 21 Jun 2020 17:56:34: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:56:34: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:56:34: #1 total tags in treatment: 22948823 INFO @ Sun, 21 Jun 2020 17:56:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:56:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:56:35: #1 tags after filtering in treatment: 22948822 INFO @ Sun, 21 Jun 2020 17:56:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:56:35: #1 finished! INFO @ Sun, 21 Jun 2020 17:56:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:56:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:56:36: #2 number of paired peaks: 56 WARNING @ Sun, 21 Jun 2020 17:56:36: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:56:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111787/SRX111787.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。