Job ID = 6453309 SRX = SRX111786 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:10:48 prefetch.2.10.7: 1) Downloading 'SRR390235'... 2020-06-21T08:10:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:16:25 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:16:25 prefetch.2.10.7: 1) 'SRR390235' was downloaded successfully Read 18970395 spots for SRR390235/SRR390235.sra Written 18970395 spots for SRR390235/SRR390235.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:32 18970395 reads; of these: 18970395 (100.00%) were unpaired; of these: 359112 (1.89%) aligned 0 times 15141528 (79.82%) aligned exactly 1 time 3469755 (18.29%) aligned >1 times 98.11% overall alignment rate Time searching: 00:04:32 Overall time: 00:04:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4050077 / 18611283 = 0.2176 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:26:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:26:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:26:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:26:23: 1000000 INFO @ Sun, 21 Jun 2020 17:26:29: 2000000 INFO @ Sun, 21 Jun 2020 17:26:35: 3000000 INFO @ Sun, 21 Jun 2020 17:26:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:26:46: 5000000 INFO @ Sun, 21 Jun 2020 17:26:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:26:47: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:26:47: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:26:52: 6000000 INFO @ Sun, 21 Jun 2020 17:26:54: 1000000 INFO @ Sun, 21 Jun 2020 17:26:59: 7000000 INFO @ Sun, 21 Jun 2020 17:27:00: 2000000 INFO @ Sun, 21 Jun 2020 17:27:05: 8000000 INFO @ Sun, 21 Jun 2020 17:27:07: 3000000 INFO @ Sun, 21 Jun 2020 17:27:11: 9000000 INFO @ Sun, 21 Jun 2020 17:27:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:27:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:27:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:27:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:27:18: 10000000 INFO @ Sun, 21 Jun 2020 17:27:20: 5000000 INFO @ Sun, 21 Jun 2020 17:27:24: 1000000 INFO @ Sun, 21 Jun 2020 17:27:24: 11000000 INFO @ Sun, 21 Jun 2020 17:27:26: 6000000 INFO @ Sun, 21 Jun 2020 17:27:31: 2000000 INFO @ Sun, 21 Jun 2020 17:27:31: 12000000 INFO @ Sun, 21 Jun 2020 17:27:33: 7000000 INFO @ Sun, 21 Jun 2020 17:27:37: 3000000 INFO @ Sun, 21 Jun 2020 17:27:38: 13000000 INFO @ Sun, 21 Jun 2020 17:27:39: 8000000 INFO @ Sun, 21 Jun 2020 17:27:44: 4000000 INFO @ Sun, 21 Jun 2020 17:27:44: 14000000 INFO @ Sun, 21 Jun 2020 17:27:46: 9000000 INFO @ Sun, 21 Jun 2020 17:27:48: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:27:48: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:27:48: #1 total tags in treatment: 14561206 INFO @ Sun, 21 Jun 2020 17:27:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:27:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:27:48: #1 tags after filtering in treatment: 14561203 INFO @ Sun, 21 Jun 2020 17:27:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:27:48: #1 finished! INFO @ Sun, 21 Jun 2020 17:27:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:27:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:27:49: #2 number of paired peaks: 63 WARNING @ Sun, 21 Jun 2020 17:27:49: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:27:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Sun, 21 Jun 2020 17:27:50: 5000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:27:52: 10000000 INFO @ Sun, 21 Jun 2020 17:27:56: 6000000 INFO @ Sun, 21 Jun 2020 17:27:58: 11000000 INFO @ Sun, 21 Jun 2020 17:28:02: 7000000 INFO @ Sun, 21 Jun 2020 17:28:04: 12000000 INFO @ Sun, 21 Jun 2020 17:28:08: 8000000 INFO @ Sun, 21 Jun 2020 17:28:11: 13000000 INFO @ Sun, 21 Jun 2020 17:28:14: 9000000 INFO @ Sun, 21 Jun 2020 17:28:17: 14000000 INFO @ Sun, 21 Jun 2020 17:28:20: 10000000 INFO @ Sun, 21 Jun 2020 17:28:20: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:28:20: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:28:20: #1 total tags in treatment: 14561206 INFO @ Sun, 21 Jun 2020 17:28:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:28:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:28:21: #1 tags after filtering in treatment: 14561203 INFO @ Sun, 21 Jun 2020 17:28:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:28:21: #1 finished! INFO @ Sun, 21 Jun 2020 17:28:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:28:21: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:28:22: #2 number of paired peaks: 63 WARNING @ Sun, 21 Jun 2020 17:28:22: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:28:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:28:26: 11000000 INFO @ Sun, 21 Jun 2020 17:28:32: 12000000 INFO @ Sun, 21 Jun 2020 17:28:37: 13000000 INFO @ Sun, 21 Jun 2020 17:28:43: 14000000 INFO @ Sun, 21 Jun 2020 17:28:47: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:28:47: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:28:47: #1 total tags in treatment: 14561206 INFO @ Sun, 21 Jun 2020 17:28:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:28:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:28:47: #1 tags after filtering in treatment: 14561203 INFO @ Sun, 21 Jun 2020 17:28:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:28:47: #1 finished! INFO @ Sun, 21 Jun 2020 17:28:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:28:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:28:48: #2 number of paired peaks: 63 WARNING @ Sun, 21 Jun 2020 17:28:48: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:28:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111786/SRX111786.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。