Job ID = 6453307 SRX = SRX111785 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:04:38 prefetch.2.10.7: 1) Downloading 'SRR390233'... 2020-06-21T08:04:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:09:12 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:09:12 prefetch.2.10.7: 1) 'SRR390233' was downloaded successfully Read 19728174 spots for SRR390233/SRR390233.sra Written 19728174 spots for SRR390233/SRR390233.sra 2020-06-21T08:10:15 prefetch.2.10.7: 1) Downloading 'SRR390234'... 2020-06-21T08:10:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:14:21 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:14:21 prefetch.2.10.7: 1) 'SRR390234' was downloaded successfully Read 24349077 spots for SRR390234/SRR390234.sra Written 24349077 spots for SRR390234/SRR390234.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:45 44077251 reads; of these: 44077251 (100.00%) were unpaired; of these: 13265131 (30.10%) aligned 0 times 25549456 (57.97%) aligned exactly 1 time 5262664 (11.94%) aligned >1 times 69.90% overall alignment rate Time searching: 00:07:45 Overall time: 00:07:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8871193 / 30812120 = 0.2879 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:30:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:30:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:30:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:30:15: 1000000 INFO @ Sun, 21 Jun 2020 17:30:20: 2000000 INFO @ Sun, 21 Jun 2020 17:30:26: 3000000 INFO @ Sun, 21 Jun 2020 17:30:31: 4000000 INFO @ Sun, 21 Jun 2020 17:30:36: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:30:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:30:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:30:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:30:42: 6000000 INFO @ Sun, 21 Jun 2020 17:30:46: 1000000 INFO @ Sun, 21 Jun 2020 17:30:48: 7000000 INFO @ Sun, 21 Jun 2020 17:30:52: 2000000 INFO @ Sun, 21 Jun 2020 17:30:54: 8000000 INFO @ Sun, 21 Jun 2020 17:30:58: 3000000 INFO @ Sun, 21 Jun 2020 17:31:00: 9000000 INFO @ Sun, 21 Jun 2020 17:31:04: 4000000 INFO @ Sun, 21 Jun 2020 17:31:06: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:31:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:31:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:31:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:31:10: 5000000 INFO @ Sun, 21 Jun 2020 17:31:12: 11000000 INFO @ Sun, 21 Jun 2020 17:31:16: 1000000 INFO @ Sun, 21 Jun 2020 17:31:16: 6000000 INFO @ Sun, 21 Jun 2020 17:31:18: 12000000 INFO @ Sun, 21 Jun 2020 17:31:22: 2000000 INFO @ Sun, 21 Jun 2020 17:31:22: 7000000 INFO @ Sun, 21 Jun 2020 17:31:24: 13000000 INFO @ Sun, 21 Jun 2020 17:31:28: 3000000 INFO @ Sun, 21 Jun 2020 17:31:28: 8000000 INFO @ Sun, 21 Jun 2020 17:31:30: 14000000 INFO @ Sun, 21 Jun 2020 17:31:34: 9000000 INFO @ Sun, 21 Jun 2020 17:31:34: 4000000 INFO @ Sun, 21 Jun 2020 17:31:36: 15000000 INFO @ Sun, 21 Jun 2020 17:31:40: 10000000 INFO @ Sun, 21 Jun 2020 17:31:41: 5000000 INFO @ Sun, 21 Jun 2020 17:31:42: 16000000 INFO @ Sun, 21 Jun 2020 17:31:46: 11000000 INFO @ Sun, 21 Jun 2020 17:31:47: 6000000 INFO @ Sun, 21 Jun 2020 17:31:48: 17000000 INFO @ Sun, 21 Jun 2020 17:31:52: 12000000 INFO @ Sun, 21 Jun 2020 17:31:53: 7000000 INFO @ Sun, 21 Jun 2020 17:31:55: 18000000 INFO @ Sun, 21 Jun 2020 17:31:59: 13000000 INFO @ Sun, 21 Jun 2020 17:31:59: 8000000 INFO @ Sun, 21 Jun 2020 17:32:01: 19000000 INFO @ Sun, 21 Jun 2020 17:32:05: 14000000 INFO @ Sun, 21 Jun 2020 17:32:05: 9000000 INFO @ Sun, 21 Jun 2020 17:32:07: 20000000 INFO @ Sun, 21 Jun 2020 17:32:11: 15000000 INFO @ Sun, 21 Jun 2020 17:32:11: 10000000 INFO @ Sun, 21 Jun 2020 17:32:13: 21000000 INFO @ Sun, 21 Jun 2020 17:32:17: 16000000 INFO @ Sun, 21 Jun 2020 17:32:18: 11000000 INFO @ Sun, 21 Jun 2020 17:32:19: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:32:19: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:32:19: #1 total tags in treatment: 21940927 INFO @ Sun, 21 Jun 2020 17:32:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:32:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:32:20: #1 tags after filtering in treatment: 21940927 INFO @ Sun, 21 Jun 2020 17:32:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:32:20: #1 finished! INFO @ Sun, 21 Jun 2020 17:32:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:32:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:32:21: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 17:32:21: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:32:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:32:23: 17000000 INFO @ Sun, 21 Jun 2020 17:32:24: 12000000 INFO @ Sun, 21 Jun 2020 17:32:29: 18000000 INFO @ Sun, 21 Jun 2020 17:32:30: 13000000 INFO @ Sun, 21 Jun 2020 17:32:35: 19000000 INFO @ Sun, 21 Jun 2020 17:32:36: 14000000 INFO @ Sun, 21 Jun 2020 17:32:41: 20000000 INFO @ Sun, 21 Jun 2020 17:32:42: 15000000 INFO @ Sun, 21 Jun 2020 17:32:47: 21000000 INFO @ Sun, 21 Jun 2020 17:32:48: 16000000 INFO @ Sun, 21 Jun 2020 17:32:53: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:32:53: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:32:53: #1 total tags in treatment: 21940927 INFO @ Sun, 21 Jun 2020 17:32:53: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:32:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:32:54: #1 tags after filtering in treatment: 21940927 INFO @ Sun, 21 Jun 2020 17:32:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:32:54: #1 finished! INFO @ Sun, 21 Jun 2020 17:32:54: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:32:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:32:54: 17000000 INFO @ Sun, 21 Jun 2020 17:32:55: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 17:32:55: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:32:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:33:00: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:33:06: 19000000 INFO @ Sun, 21 Jun 2020 17:33:11: 20000000 INFO @ Sun, 21 Jun 2020 17:33:17: 21000000 INFO @ Sun, 21 Jun 2020 17:33:23: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:33:23: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:33:23: #1 total tags in treatment: 21940927 INFO @ Sun, 21 Jun 2020 17:33:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:33:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:33:24: #1 tags after filtering in treatment: 21940927 INFO @ Sun, 21 Jun 2020 17:33:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:33:24: #1 finished! INFO @ Sun, 21 Jun 2020 17:33:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:33:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:33:25: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 17:33:25: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:33:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111785/SRX111785.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。