Job ID = 6453303 SRX = SRX111781 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:05:39 prefetch.2.10.7: 1) Downloading 'SRR390226'... 2020-06-21T08:05:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:12:13 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:12:13 prefetch.2.10.7: 1) 'SRR390226' was downloaded successfully Read 24820199 spots for SRR390226/SRR390226.sra Written 24820199 spots for SRR390226/SRR390226.sra 2020-06-21T08:13:32 prefetch.2.10.7: 1) Downloading 'SRR390227'... 2020-06-21T08:13:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:16:56 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:16:56 prefetch.2.10.7: 1) 'SRR390227' was downloaded successfully Read 19301994 spots for SRR390227/SRR390227.sra Written 19301994 spots for SRR390227/SRR390227.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:11 44122193 reads; of these: 44122193 (100.00%) were unpaired; of these: 4208749 (9.54%) aligned 0 times 31381105 (71.12%) aligned exactly 1 time 8532339 (19.34%) aligned >1 times 90.46% overall alignment rate Time searching: 00:09:11 Overall time: 00:09:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13410720 / 39913444 = 0.3360 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:35:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:35:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:35:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:35:54: 1000000 INFO @ Sun, 21 Jun 2020 17:35:59: 2000000 INFO @ Sun, 21 Jun 2020 17:36:05: 3000000 INFO @ Sun, 21 Jun 2020 17:36:10: 4000000 INFO @ Sun, 21 Jun 2020 17:36:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:36:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:36:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:36:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:36:22: 6000000 INFO @ Sun, 21 Jun 2020 17:36:24: 1000000 INFO @ Sun, 21 Jun 2020 17:36:28: 7000000 INFO @ Sun, 21 Jun 2020 17:36:31: 2000000 INFO @ Sun, 21 Jun 2020 17:36:34: 8000000 INFO @ Sun, 21 Jun 2020 17:36:37: 3000000 INFO @ Sun, 21 Jun 2020 17:36:40: 9000000 INFO @ Sun, 21 Jun 2020 17:36:43: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:36:47: 10000000 INFO @ Sun, 21 Jun 2020 17:36:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:36:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:36:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:36:49: 5000000 INFO @ Sun, 21 Jun 2020 17:36:53: 11000000 INFO @ Sun, 21 Jun 2020 17:36:54: 1000000 INFO @ Sun, 21 Jun 2020 17:36:56: 6000000 INFO @ Sun, 21 Jun 2020 17:36:59: 12000000 INFO @ Sun, 21 Jun 2020 17:37:01: 2000000 INFO @ Sun, 21 Jun 2020 17:37:02: 7000000 INFO @ Sun, 21 Jun 2020 17:37:05: 13000000 INFO @ Sun, 21 Jun 2020 17:37:07: 3000000 INFO @ Sun, 21 Jun 2020 17:37:08: 8000000 INFO @ Sun, 21 Jun 2020 17:37:12: 14000000 INFO @ Sun, 21 Jun 2020 17:37:13: 4000000 INFO @ Sun, 21 Jun 2020 17:37:15: 9000000 INFO @ Sun, 21 Jun 2020 17:37:18: 15000000 INFO @ Sun, 21 Jun 2020 17:37:20: 5000000 INFO @ Sun, 21 Jun 2020 17:37:21: 10000000 INFO @ Sun, 21 Jun 2020 17:37:24: 16000000 INFO @ Sun, 21 Jun 2020 17:37:26: 6000000 INFO @ Sun, 21 Jun 2020 17:37:27: 11000000 INFO @ Sun, 21 Jun 2020 17:37:31: 17000000 INFO @ Sun, 21 Jun 2020 17:37:32: 7000000 INFO @ Sun, 21 Jun 2020 17:37:34: 12000000 INFO @ Sun, 21 Jun 2020 17:37:37: 18000000 INFO @ Sun, 21 Jun 2020 17:37:39: 8000000 INFO @ Sun, 21 Jun 2020 17:37:40: 13000000 INFO @ Sun, 21 Jun 2020 17:37:43: 19000000 INFO @ Sun, 21 Jun 2020 17:37:45: 9000000 INFO @ Sun, 21 Jun 2020 17:37:47: 14000000 INFO @ Sun, 21 Jun 2020 17:37:50: 20000000 INFO @ Sun, 21 Jun 2020 17:37:51: 10000000 INFO @ Sun, 21 Jun 2020 17:37:53: 15000000 INFO @ Sun, 21 Jun 2020 17:37:56: 21000000 INFO @ Sun, 21 Jun 2020 17:37:57: 11000000 INFO @ Sun, 21 Jun 2020 17:37:59: 16000000 INFO @ Sun, 21 Jun 2020 17:38:03: 22000000 INFO @ Sun, 21 Jun 2020 17:38:04: 12000000 INFO @ Sun, 21 Jun 2020 17:38:06: 17000000 INFO @ Sun, 21 Jun 2020 17:38:09: 23000000 INFO @ Sun, 21 Jun 2020 17:38:10: 13000000 INFO @ Sun, 21 Jun 2020 17:38:12: 18000000 INFO @ Sun, 21 Jun 2020 17:38:15: 24000000 INFO @ Sun, 21 Jun 2020 17:38:16: 14000000 INFO @ Sun, 21 Jun 2020 17:38:18: 19000000 INFO @ Sun, 21 Jun 2020 17:38:22: 25000000 INFO @ Sun, 21 Jun 2020 17:38:23: 15000000 INFO @ Sun, 21 Jun 2020 17:38:24: 20000000 INFO @ Sun, 21 Jun 2020 17:38:28: 26000000 INFO @ Sun, 21 Jun 2020 17:38:29: 16000000 INFO @ Sun, 21 Jun 2020 17:38:31: 21000000 INFO @ Sun, 21 Jun 2020 17:38:31: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:38:31: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:38:31: #1 total tags in treatment: 26502724 INFO @ Sun, 21 Jun 2020 17:38:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:38:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:38:32: #1 tags after filtering in treatment: 26502723 INFO @ Sun, 21 Jun 2020 17:38:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:38:32: #1 finished! INFO @ Sun, 21 Jun 2020 17:38:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:38:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:38:34: #2 number of paired peaks: 15 WARNING @ Sun, 21 Jun 2020 17:38:34: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:38:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:38:35: 17000000 INFO @ Sun, 21 Jun 2020 17:38:37: 22000000 INFO @ Sun, 21 Jun 2020 17:38:41: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:38:43: 23000000 INFO @ Sun, 21 Jun 2020 17:38:47: 19000000 INFO @ Sun, 21 Jun 2020 17:38:50: 24000000 INFO @ Sun, 21 Jun 2020 17:38:54: 20000000 INFO @ Sun, 21 Jun 2020 17:38:56: 25000000 INFO @ Sun, 21 Jun 2020 17:39:00: 21000000 INFO @ Sun, 21 Jun 2020 17:39:02: 26000000 INFO @ Sun, 21 Jun 2020 17:39:06: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:39:06: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:39:06: #1 total tags in treatment: 26502724 INFO @ Sun, 21 Jun 2020 17:39:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:39:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:39:06: #1 tags after filtering in treatment: 26502723 INFO @ Sun, 21 Jun 2020 17:39:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:39:06: #1 finished! INFO @ Sun, 21 Jun 2020 17:39:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:39:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:39:06: 22000000 INFO @ Sun, 21 Jun 2020 17:39:08: #2 number of paired peaks: 15 WARNING @ Sun, 21 Jun 2020 17:39:08: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:39:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:39:12: 23000000 INFO @ Sun, 21 Jun 2020 17:39:18: 24000000 INFO @ Sun, 21 Jun 2020 17:39:24: 25000000 INFO @ Sun, 21 Jun 2020 17:39:29: 26000000 INFO @ Sun, 21 Jun 2020 17:39:33: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 17:39:33: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 17:39:33: #1 total tags in treatment: 26502724 INFO @ Sun, 21 Jun 2020 17:39:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:39:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:39:33: #1 tags after filtering in treatment: 26502723 INFO @ Sun, 21 Jun 2020 17:39:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:39:33: #1 finished! INFO @ Sun, 21 Jun 2020 17:39:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:39:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:39:35: #2 number of paired peaks: 15 WARNING @ Sun, 21 Jun 2020 17:39:35: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 17:39:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX111781/SRX111781.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。